LOCUS       sequence-175025-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR KO of Trp73.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Wang Q, Zou Y, Nowotschin S, Kim SY, Li QV, Soh CL, Su J, Zhang C, 
            Shu W, Xi Q, Huangfu D, Hadjantonakis AK, Massague J
  TITLE     The p53 Family Coordinates Wnt and Nodal Inputs in Mesendodermal 
            Differentiation of Embryonic Stem Cells.
  JOURNAL   Cell Stem Cell. 2017 Jan 5;20(1):70-86. doi: 
            10.1016/j.stem.2016.10.002. Epub 2016 Nov 23.
  PUBMED    27889317
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.10.002"; journalName: "Cell Stem Cell"; date: 
            "2017-01-5- 5"; volume: "20"; issue: "1"; pages: "70-86"
COMMENT     Sequence Label: LentiGuidPuro-hTP73_sgRNA-1
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     501..520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     754..771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    888..909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(924..929,930..947,948..954)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    962..978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     967..989
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     986..1002
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     986..1002
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1021..1041
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1023..1041
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1069..1295
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1296..1476
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1523..1648
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2141..2374
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2559..2603
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2781..3029
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2781..2801
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2952..2971
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3051..3126
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3182..3299
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3438..4616
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3669..4607
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4564..4584
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4629..5228
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4629..4648)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5125..5145
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5244..5832
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5297..5317)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5715..5726)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5904..6137
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6215..6336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6252..6271)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6306..6325
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6376..6511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6531..6550)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6532..6550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6560..6577)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6560..6576)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6569..6591)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6718..7173
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6805..6824)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7015..7036
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7199..7303
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7304..7372,7373..8164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7522..7541)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
       61 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      781 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      841 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      901 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      961 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     1021 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     1081 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     1141 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     1201 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     1261 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     1321 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1381 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1441 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     1501 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1561 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1621 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1681 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1741 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1801 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1861 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1921 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1981 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     2041 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     2101 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     2161 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     2221 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     2281 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     2341 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     2401 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     2461 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     2521 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     2581 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2641 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2701 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2761 caaccccgag gggacccaga gagggcctat ttcccatgat tccttcatat ttgcatatac
     2821 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag
     2881 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     2941 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     3001 tatatatctt gtggaaagga cgaaacaccg gcaccacgtt tgagcacctc gttttagagc
     3061 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     3121 cggtgctttt ttaagcttgg cgtaactaga tcttgagaca aatggcagta ttcatccaca
     3181 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     3241 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     3301 gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg gcccgggtgc
     3361 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
     3421 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     3481 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
     3541 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     3601 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     3661 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     3721 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     3781 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     3841 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     3901 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     3961 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     4021 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     4081 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     4141 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     4201 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     4261 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     4321 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     4381 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     4441 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     4501 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     4561 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta
     4621 cggccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     4681 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     4741 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     4801 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca
     4861 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     4921 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     4981 ccaaggagcc cgcgtggttc ctggccaccg tcggagtctc gcccgaccac cagggcaagg
     5041 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5101 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5161 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5221 gtgcctgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     5281 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5341 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5401 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5461 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5521 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5581 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     5641 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5701 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5761 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5821 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     5881 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5941 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6001 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6061 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6121 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     6181 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     6241 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6301 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     6361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6481 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     6541 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6601 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6661 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     6721 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6781 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6841 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6901 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6961 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7021 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7081 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7141 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     7201 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7261 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7321 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7381 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7441 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7501 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7561 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7621 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7681 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7741 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7801 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7861 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7921 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7981 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8041 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8101 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8161 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8221 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8281 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atc
//
