LOCUS       sequence-175028-        8841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing UAS-3xHalo7::CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: UAS-3xHalo7::CAAX
FEATURES             Location/Qualifiers
     source          1..8841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..110
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    144..238
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             complement(join(1268..1882,1953..2084,2288..2603,2665..3319,
                     3394..3667,4069..4140))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4592..4610
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4835..4904
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(4991..5579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5071..5090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5750..6541,6542..6610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6373..6392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6611..6715)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6782..6800
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(7587..8477)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      8474..8483
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          8507..8573
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     promoter        complement(8588..8826)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(8620..8641)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 gcgctagagt ctccgctcgg aggacagtac tccgctcgga ggacagtact ccgctcggag
       61 gacagtactc cgctcggagg acagtactcc gctcggagga cagtactccg acctgcaggc
      121 atgccctagg gctagagtct ccgctcggag gacagtactc cgctcggagg acagtactcc
      181 gctcggagga cagtactccg ctcggaggac agtactccgc tcggaggaca gtactccgac
      241 ctgcaggcat gcaagcttgc ggccctggcc acgggtgcgc atgatcgtgc tcctgtcgtt
      301 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg
      361 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat
      421 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat
      481 tatgttccgg aggcgcgccc tagttccagt gaaatccaag cattttctaa attaaatgta
      541 ttcttattat tatagttgtt atttttgata tatataaaca acactattat gcccaccatt
      601 tttttgagat gcatctacac aaggaacaaa cactggatgt cactttcagt tcaaattgta
      661 acgctaatca ctccgaacag gtcacaaaaa attaccttaa aaagtcataa tattaaatta
      721 gaataaatat agctgtgagg gaaatatata caaatatatt ggagcaaata aattgtacat
      781 acaaatattt attactaatt tctattgaga cgaaatgaac cactcggaac catttgagcg
      841 aaccgaatcg cgcggaacta acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt
      901 gttgcggaga gcgggtggga gacagcgaaa gagcaactac gaaacgtggt gtggtggagg
      961 tgaattatga agagggcgcg cgatttgaaa agtatgtata taaaaaatat atcccggtgt
     1021 tttatgtagc gataaacgag tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt
     1081 agaagacaaa tccaaagtct acttgtgggg atgttcgaag gggaaatact tgtattctat
     1141 aggtcatatc ttgtttttat tggcacaaat ataattacat tagctttttg agggggcaat
     1201 aaacagtaaa cacgatggta ataatggtaa aaaaaaaaaa caagcagtta tttcggatat
     1261 atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg agcacccgga
     1321 agctcacgat gagaatggcc agacccacgt agtccagcgg cagatcggcg gcggagaagt
     1381 taagcgtctc caggatgacc ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc
     1441 taatttcgcc cggctccacg tccgcccatt ggttaatcag cagaccctcg ttggcgtaac
     1501 ggaaccatga gaggtacgac aaccatttga ggtatactgg caccgagccc gagttcaaga
     1561 agaagccgcc aaagagcagg aatggtatga taaccggcgg acccacagac agcgccatcg
     1621 aggtcgagga gctggcgcag gatattagat atccgaagga cgttgacaca ttggccacca
     1681 gagtgaccag cgccaggcag ttgaagaagt gcagcactcc ggcccgcagt ccgatcatcg
     1741 gataggcaat cgccgtgaag accagtggca ctgtgagaaa aagcggcaat tcggcaatcg
     1801 ttttgcccag aaagtatgtg tcacagcgat aaagtcgact tcgggcctcc ctcataaaaa
     1861 ctggcagctc tgaggtgaac acctaaatcg aatcgattca ttagaaagtt agtaaattat
     1921 tgaaatgcaa atgtattcta aacatgactt acatttatcg tggcaaagac gttttgaaag
     1981 gtcatgttgg tcaggaagag gaagatggct ccgttgatat tcatcacacc cacttgcgtg
     2041 agttgttggc ccaaaaagat gaggccaatc aagatggcaa ccatctgcaa attaaaatgt
     2101 tactcgcatc tcattaatat tcgcgagtta aatgaaattt atttatcttc tgcaaaacta
     2161 taaactatac atctcattga aaaaaactaa gaagggtgtg gaatcaggca attctatcta
     2221 aaatctagcg aatttgtttc caagaattgt aagcgttata tcatttgttt ccactggaac
     2281 cactcaccgt tgtctgaata agtcgcactt ttacgaggag tggttccttg agcaccgaca
     2341 gccaggatcg ccacaggacc gcccggaact gcatgaacca ggtggccttg taggtgtacc
     2401 cattctccgg ctgctccagt ggcttctcca gatttttggt ggccaacaac tgctccatat
     2461 cccgggctac tttgctaatg gcaaaattgt cgcatatctt ggcgatccga tcacgggact
     2521 cgatctcccg tccgggcaca acggccaaca cctgtacgta aaagtccgcc ggattgtagt
     2581 tggtaggaca ctgggcaccc acgctggata ggagttgaga tgttatgtaa tactagatac
     2641 ccttaataaa cacatcgaac tcactaggaa aagaagtcga cggcttcgct gggagtgccc
     2701 aagaaagcta ccctgccctc ggccatcaga aggatcttgt caaagagctc aaacagctcg
     2761 gaagacggct gatgaatggt caggatgacg gtcttgccct tctgcgacag cttcttcagc
     2821 acctggacga cgctgtgggc ggtaaaggag tccagtccgg aggtgggctc atcgcagatc
     2881 agaagcggcg gatcggttag agcctcggag gcgaatgcca gacgcttcct ttctccgccg
     2941 gacagacctt tcaccctgcc gggcacaccg atgatcgtgt gctgacattt gctgagcgaa
     3001 agctcctgga tcacctgatc cacgcgggcc actcgctgcc gataggtcag atgtcgtggc
     3061 atccgcacca tggcttggaa aatcaggtgt tccctggccg ttagggagcc gataaagagg
     3121 tcatcctgct ggacataggc gcacctggcc tgcatctcct tggcgtccac aggttggcca
     3181 ttgagcagtc gcatcccgga tggcgatact tggatgccct gcggcgatcg aaaggcaagg
     3241 gcattcagca gggtcgtctt tccggcaccg gaactgccca tcacggccaa aagttcgccc
     3301 ggataggcca cgccgcaaac tgagtttcaa attggtaatt ggacccttta ttaagatttc
     3361 acacagatca gccgactgcg aatagaaact caccgttctt gagcaaatgt ttcctgggcg
     3421 ccggtatgtg tcgctcgttg cagaatagtc cgcgtgtccg gttgaccagc tgccgccatc
     3481 cggagcccgg ctgattgacc gccccaaaga tgtccatatt gtgccaggca taggtgaggt
     3541 tctcggctag ttggccgctc cctgaaccgg agtcctccgg cggactgggt ggccggagcg
     3601 tgccgtagtt tttggcctgc ccgaagccct ggttaatgca gctctgcgaa gccgctccgc
     3661 tgtcaccctg caatgatagg ggatctcaaa tatcaactac aagcgttatg ctcatctaac
     3721 cccgaacaaa aagtaccccg aagtatccta cgaagtaggt ttatactttt atttattttt
     3781 tgtgcatcta ggatcagctt aaaatatctg gttgttatat tttttgtaaa aaagaatata
     3841 gtcgaaaatg aatgccttta gatgtcttga tcatgatatg atctcaaaaa ttgtcttata
     3901 tagcgagaac agctaccaga ataatctgtt tcgtgtcact atttgtttgt gcaattgcgg
     3961 tttgggattt ttgtgggtcg cagttctcac gccgcagaca atttgatgtt gcaatcgcag
     4021 ttcctataga tcaagtgaac ttaagatgta tgcacatgta ctactcacat tgttcagatg
     4081 ctcggcagat gggtgtttgc tgcctccgcg aattaatagc tcctgatcct cttggcccat
     4141 tgccgggatt tttcacactt tcccctgctt acccacccaa aaccaatcac caccccaatc
     4201 actcaaaaaa caaacaaaaa taagaagcga gaggagtttt ggcacagcac tttgtgttta
     4261 attgatggcg taaaccgctt ggagcttcgt cacgaaaccg ctgacaaaat gcaactgaag
     4321 gcggacattg acgctacgta acgctacaaa cggtggcgaa agagatagcg gacgcagcgg
     4381 cgaaagagac ggcgatattt ctgtggacag agaaggaggc aaacagcgct gactttgagt
     4441 ggaatgtcat tttgagtgag aggtaatcga aagaacctgg tacatcaaat acccttggat
     4501 cgaagtaaat ttaaaactga tcagataagt tcaatgatat ccagtgcagt aaaaaaaaaa
     4561 aatgtttttt ttatctactt tccgcaaaaa tgggttttat taacttacat acatactagg
     4621 cgcgcccata tgttcggctt gtcgacatgc ccgccgtgac cgtcgagaac ccgctgacgc
     4681 tgccccgcgt atccgcaccc gccgacgccg tcgcacgtcc cgtgctcacc gtgaccaccg
     4741 cgcccagcgg tttcgagggc gagggcttcc cggtgcgccg cgcgttcgcc gggatcaact
     4801 accgccacct cgacccgttc atcatgatgg accagatggg tgaggtggag tacgcgcccg
     4861 gggagcccaa gggcacgccc tggcacccgc accgcggctt cgagaccgtg acctacatcg
     4921 tcgacggtaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4981 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5041 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5161 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5341 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5401 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     5461 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5521 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5581 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5641 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5701 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5761 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5821 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5881 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5941 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6001 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6061 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6121 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6181 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6241 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6301 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6361 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6421 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6481 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6541 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6601 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6661 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6721 ttccccgaaa agtgccacct gagtctaaga aaccattatt atcatgacat taacctataa
     6781 aaataggcgt atcacgaggc cctttcgtct tcaagaattc gtttaaacgg ccggccgtta
     6841 actcgaatcg ctatccaagc cagctccatt gtcggcatcg tgctcattct attgacgctg
     6901 gcagatttgg ttttggcgct atgggacccg ttcggttaca acaacatgtt tccgcgcgag
     6961 tttcccgacg acatgtcgcg cacgttcctg actgcgtact ttgagagttt cgacaacacc
     7021 acgtccagag aaatcataga gtttatgccc gagttctttt cggaaatggt cgaaacggac
     7081 gatgacgcca cgtttgaatc tctatttcat ttattagatt atgtggcatc tttagaagtt
     7141 aattccgacg gccaaatgtt aaacttggag gagggtgatg aaattgagga ttttgacgaa
     7201 tctactttgg tggggcaagc gttagccact agctcgctat acactcgcat ggagtttatg
     7261 cagtacacgt ttaggcaaaa cacactattg tctatgaaca aagaaaacaa caattttaat
     7321 caaataatca tgggtttatt tgcaacaaac acaattgtgg cgtttacagc atttgttata
     7381 cacacagaac tcatattttt tatatttttc gtaatcttcc taatgatcac attttattac
     7441 ataatcaaag aatcgtacga atattataaa acaattgatt tgttatttta aaaacgattc
     7501 attctagatt acataattac acactttgtc tttgacttct ttttcttctt tttaccatct
     7561 ttgctcatct cgagactagt agatctgccg gaaatttcga gcgtcgacag ccagcgcgcg
     7621 atctcgctgc cgatcaggtc cgggttgtct tcttgcagca gattcagacc cgggccgatg
     7681 tccacagcct tgcagttagg caggcttttg gccaggcgag cggcttcggc cggtgggatc
     7741 agaacgcctg gggtgcccca gaacagcagc ttcgggacag gggactggtg cagccagtcc
     7801 atgtattctt cgaccagcgc gacgatgttc gctggctcac cggcgattgg cagctcgttt
     7861 gggaagcgcc acagtggctc gcggtcaaca ggattcagga acggctcgcg gtaatggtcc
     7921 atctcgactt cagtcagcgg gcggacgaca cccatcggca gcgtaccctc gataaaaacg
     7981 ttctgatcga tgatcagctt gcggccgacg tcggtggtgc ggaaggcctg gaaggtctcg
     8041 cgggcaaatt ctggccattc gtcccaggtc gggatagggc ggatgaactc cataaatgca
     8101 atacctttga cgcgctctgg attgcgcttg gcccagtgga aacccagagc ggagccccag
     8161 tcgtgaatga ccaggacgac ctcttccaga cccagggctt cgatgaaggc atccatgaag
     8221 cggacgtggt cgtcgaagaa ataacccagg tctggtttgt cggatttgcc cataccgatc
     8281 aggtctggag caatgcagcg atgggtcggt gcaacatgcg ggatgatgtt gcgccacacg
     8341 taggaggagg tcgggttacc gtgcaggaac agcacagggg tgccatcgcg cggaccaaca
     8401 tcgacgtagt gcatgcgctc gcccaggact tccacataat gggggtcgaa tggaaagcca
     8461 gtaccgattt ctgccatggt ggcggttttt taggagttgc ggccgcctga agtaaaggat
     8521 aagaattagg gttagtcgtt tcggtgtgcc taggcatcag tggttgaacc taccttttag
     8581 atctattcag agttctcttc ttgtattcaa taattacttc ttggcagatt tcagtagttg
     8641 cagttgattt acttggttgc tggttacttt taattgattc actttaactt gcactttact
     8701 gcagattgtt tagcttgttc agctgcgctt gtttatttgc ttagctttcg cttagcgacg
     8761 tgttcacttt gcttgtttga attgaattgt cgctccgtag acgaagcgcc tctatttata
     8821 ctccggcgct cgacgtcgct a
//
