LOCUS       sequence-17503-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Murphy LO, Smith S, Chen RH, Fingar DC, Blenis J
  TITLE     Molecular interpretation of ERK signal duration by immediate early 
            gene products.
  JOURNAL   Nat Cell Biol. 2002 Aug . 4(8):556-64.
  PUBMED    12134156
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2002 Aug . 4(8):556-64."
COMMENT     Sequence Label: pcDNA3-FLAG-Fos DD T325A T331A
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             126..149
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
       61 acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattca
      121 ccatggacta caaagacgat gacgacaagt tctcgggttt caacgcggac tacgaggcgt
      181 catcctcccg ctgcagtagc gcctccccgg ccggggacag cctttcctac taccattccc
      241 cagccgactc cttctccagc atgggctccc ctgtcaacac acaggacttt tgcgcagatc
      301 tgtccgtctc tagtgccaac tttatcccca cggtgacagc catctccacc agcccagacc
      361 tgcagtggct ggtgcagccc actctggtct cctccgtggc cccatcgcag accagagcgc
      421 cccatcctta cggactcccc accccgtcga ccggggctta cgccagagcg ggagtggtga
      481 agaccatgtc aggcggcaga gcgcagagca tcggcagaag gggcaaagta gagcagctat
      541 ctcctgaaga ggaagagaaa cggagaatcc gaagggaaag gaataagatg gctgcagcca
      601 agtgccggaa tcggaggagg gagctgacag atacgctcca agcggagaca gatcaacttg
      661 aagacgagaa gtctgcgttg cagaccgaga ttgccaatct actgaaagag aaggaaaaac
      721 tggagtttat tttggcagcc caccgacctg cctgcaagat ccccaatgac ctgggcttcc
      781 cagaggagat gtctgtgacc tccctggact tgactggggg tctgcctgag gctaccaccc
      841 cagagtctga ggaggccttc accctgc
//
