LOCUS       sequence-175031-        3910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mouse Trex2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Yumlu S, Bashir S, Stumm J, Kuhn R
  TITLE     Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using
            CRISPR/Cas9.
  JOURNAL   Methods Mol Biol. 2019;1961:137-151. doi: 
            10.1007/978-1-4939-9170-9_10.
  PUBMED    30912045
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2019"; volume: "1961:137-151. doi"; pages: " 
            10.1007/978-1-4939-9170-9_10"
COMMENT     Sequence Label: pCAG-Trex2-bpA
FEATURES             Location/Qualifiers
     source          1..3910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(813..829)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(843..860)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    849..1073
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1145..1162)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1145..1161)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1154..1176)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(1302..1757)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1439..1460)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1651..1670
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1784..1888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1889..1957,1958..2749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2107..2126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2920..3508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3409..3428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3662..3679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3796..3817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3832..3837,3838..3855,3856..3862)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3870..3886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3875..3897
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3894..3910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3894..3910
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta attaataata
       61 cgactcacta taggggccgc caccatgtct gagccacccc gggctgagac ctttgtattc
      121 ctggacctag aagccactgg gctcccaaac atggatcctg agattgcaga gatatccctt
      181 tttgctgttc accgctcttc cctggagaac ccagaacggg atgattctgg ttccttggtg
      241 ctgccccgtg ttctggacaa gctcacactg tgcatgtgcc cggagcgccc ctttactgcc
      301 aaggccagtg agattactgg tttgagcagc gaaagcctga tgcactgcgg gaaggctggt
      361 ttcaatggcg ctgtggtaag gacactgcag ggcttcctaa gccgccagga gggccccatc
      421 tgccttgtgg cccacaatgg cttcgattat gacttcccac tgctgtgcac ggagctacaa
      481 cgtctgggtg cccatctgcc ccaagacact gtctgcctgg acacactgcc tgcattgcgg
      541 ggcctggacc gtgctcacag ccacggcacc agggctcaag gccgcaaaag ctacagcctg
      601 gccagtctct tccaccgcta cttccaggct gaacccagtg ctgcccattc agcagaaggt
      661 gatgtgcaca ccctgcttct gatcttcctg catcgtgctc ctgagctgct cgcctgggca
      721 gatgagcagg cccgcagctg ggctcatatt gagcccatgt acgtgccacc tgatggtcca
      781 agcctcgaag cctgaacgcg taaatgattg cagatccact agttctagag ctcgctgatc
      841 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      901 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      961 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     1021 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     1081 ggcggaaaga accagctggg gctcgagatc cactagttct agcctcgagg ctagagcggc
     1141 cgccactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     1201 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     1261 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat
     1321 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     1381 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     1441 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     1501 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     1561 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     1621 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     1681 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     1741 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1801 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1861 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1921 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1981 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2041 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2101 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2161 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2221 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2281 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2341 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     2401 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     2461 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     2521 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2581 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     2641 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2701 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     2761 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2821 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2941 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3061 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3181 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3241 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3301 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3361 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3421 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3481 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3541 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3601 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3661 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     3721 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3781 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     3841 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     3901 cagctatgac
//
