LOCUS       sequence-17510-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Dimitri CA, Dowdle W, MacKeigan JP, Blenis J, Murphy LO
  TITLE     Spatially separate docking sites on ERK2 regulate distinct signaling
            events in vivo.
  JOURNAL   Curr Biol. 2005 Jul 26. 15(14):1319-24.
  PUBMED    16051177
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2005 Jul 26. 15(14):1319-24."
COMMENT     Sequence Label: pcDNA3-HA-ERK2 Y261A
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcttagcgac tctctggcta ctgagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cacgcgtcat atggcttacc catacgatgt
      121 tccagattac gctagcatgg cggcggcggc ggcgggcccg gagatggtcc gcgggcaggt
      181 gttcgacgtg gggccgcgct acactaatct ctcgtacatc ggagaaggcg cctacggcat
      241 ggtttgttct gcttatgata atctcaacaa agttcgagtt gctatcaaga aaatcagtcc
      301 ttttgagcac cagacctact gtcagagaac cctgagagag ataaaaatcc tactgcgctt
      361 cagacatgag aacatcatcg gcatcaatga catcatccgg gcaccaacca ttgagcagat
      421 gaaagatgta tatatagtac aggacctcat ggagacagat ctttacaagc tcttgaagac
      481 acagcacctc agcaatgatc atatctgcta ttttctttat cagatcctga gaggattaaa
      541 gtatatacat tcagctaatg ttctgcaccg tgacctcaag ccttccaacc tcctgctgaa
      601 caccacttgt gatctcaaga tctgtgactt tggccttgcc cgtgttgcag atccagacca
      661 tgatcataca gggttcttga cagagtatgt agccacgcgt tggtacagag ctccagaaat
      721 tatgttgaat tccaagggtt ataccaagtc cattgatatt tggtctgtgg gctgcatcct
      781 ggcagagatg ctatccaaca ggcctatctt cccaggaaag cattaccttg accagctgaa
      841 tcacatcctg ggtattcttg gatctccatc acaggaagat ctgaattgt
//
