LOCUS       sequence-175124-       13113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To produce Lentiviral vector for gene editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard 
            G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, 
            Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, 
            du Pasquier R, Deglon N
  TITLE     The Self-Inactivating KamiCas9 System for the Editing of CNS Disease
            Genes.
  JOURNAL   Cell Rep. 2017 Sep 19;20(12):2980-2991. doi: 
            10.1016/j.celrep.2017.08.075.
  PUBMED    28930690
REFERENCE   2  (bases 1 to 13113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-19- 19"; volume: "20"; issue: "12"; pages: "2980-2991"
COMMENT     Sequence Label: SIN-PGK-Cas9-WPRE
FEATURES             Location/Qualifiers
     source          1..13113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2163..2662
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2198..2221)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2595..2614
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2673..2689
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2673..2689
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    2711..2735
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2741..6844
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2741..6844
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2741..6844
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6857..6877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6921..6945)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6968..6984)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6969..6985)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6998..7586
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7051..7071)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7469..7480)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7675..7908
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7933..7951)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7934..7951)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8241..8260)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8474..8493)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8593..8615
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8653..8671)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8739..8843
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8844..8912,8913..9704)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9062..9081)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9875..10463
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10364..10383
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10617..10634
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10704..10724)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10709..11038
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10889..11024
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12172..12237
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12367..12387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12812..12946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12849..12868)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12903..12922
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     2041 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2101 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tggtcgagta
     2161 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     2221 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     2281 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     2341 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     2401 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     2461 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     2521 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     2581 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     2641 ttcctcatct ccgggccttt cgacctctag gatctagaac tagtggatcc cccgggctgc
     2701 aggaattatc acaagtttgt acaaaaaagc aggctccacc atggacaaga agtactccat
     2761 tgggctcgat atcggcacaa acagcgtcgg ctgggccgtc attacggacg agtacaaggt
     2821 gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa agaagaacct
     2881 cattggcgcc ctcctgttcg actccgggga gacggccgaa gccacgcggc tcaaaagaac
     2941 agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg agatctttag
     3001 taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt cctttttggt
     3061 ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg acgaggtggc
     3121 gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagcttgtag acagtactga
     3181 taaggctgac ttgcggttga tctatctcgc gctggcgcat atgatcaaat ttcggggaca
     3241 cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac tctttatcca
     3301 actggttcag acttacaatc agcttttcga agagaacccg atcaacgcat ccggagttga
     3361 cgccaaagca atcctgagcg ctaggctgtc caaatcccgg cggctcgaaa acctcatcgc
     3421 acagctccct ggggagaaga agaacggcct gtttggtaat cttatcgccc tgtcactcgg
     3481 gctgaccccc aactttaaat ctaacttcga cctggccgaa gatgccaagc ttcaactgag
     3541 caaagacacc tacgatgatg atctcgacaa tctgctggcc cagatcggcg accagtacgc
     3601 agaccttttt ttggcggcaa agaacctgtc agacgccatt ctgctgagtg atattctgcg
     3661 agtgaacacg gagatcacca aagctccgct gagcgctagt atgatcaagc gctatgatga
     3721 gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga cagcaactgc ctgagaagta
     3781 caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc ggatacattg acggcggagc
     3841 aagccaggag gaattttaca aatttattaa gcccatcttg gaaaaaatgg acggcaccga
     3901 ggagctgctg gtaaagctta acagagaaga tctgttgcgc aaacagcgca ctttcgacaa
     3961 tggaagcatc ccccaccaga ttcacctggg cgaactgcac gctatcctca ggcggcaaga
     4021 ggatttctac ccctttttga aagataacag ggaaaagatt gagaaaatcc tcacatttcg
     4081 gataccctac tatgtaggcc ccctcgcccg gggaaattcc agattcgcgt ggatgactcg
     4141 caaatcagaa gagaccatca ctccctggaa cttcgaggaa gtcgtggata agggggcctc
     4201 tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa aatctgccta acgaaaaggt
     4261 gcttcctaaa cactctctgc tgtacgagta cttcacagtt tataacgagc tcaccaaggt
     4321 caaatacgtc acagaaggga tgagaaagcc agcattcctg tctggagagc agaagaaagc
     4381 tatcgtggac ctcctcttca agacgaaccg gaaagttacc gtgaaacagc tcaaagaaga
     4441 ctatttcaaa aagattgaat gtttcgactc tgttgaaatc agcggagtgg aggatcgctt
     4501 caacgcatcc ctgggaacgt atcacgatct cctgaaaatc attaaagaca aggacttcct
     4561 ggacaatgag gagaacgagg acattcttga ggacattgtc ctcaccctta cgttgtttga
     4621 agatagggag atgattgaag aacgcttgaa aacttacgct catctcttcg acgacaaagt
     4681 catgaaacag ctcaagaggc gccgatatac aggatggggg cggctgtcaa gaaaactgat
     4741 caatgggatc cgagacaagc agagtggaaa gacaatcctg gattttctta agtccgatgg
     4801 atttgccaac cggaacttca tgcagttgat ccatgatgac tctctcacct ttaaggagga
     4861 catccagaaa gcacaagttt ctggccaggg ggacagtctt cacgagcaca tcgctaatct
     4921 tgcaggtagc ccagctatca aaaagggaat actgcagacc gttaaggtcg tggatgaact
     4981 cgtcaaagta atgggaaggc ataagcccga gaatatcgtt atcgagatgg cccgagagaa
     5041 ccaaactacc cagaagggac agaagaacag tagggaaagg atgaagagga ttgaagaggg
     5101 tataaaagaa ctggggtccc aaatccttaa ggaacaccca gttgaaaaca cccagcttca
     5161 gaatgagaag ctctacctgt actacctgca gaacggcagg gacatgtacg tggatcagga
     5221 actggacatc aatcggctct ccgactacga cgtggatcat atcgtgcccc agtcttttct
     5281 caaagatgat tctattgata ataaagtgtt gacaagatcc gataaaaata gagggaagag
     5341 tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa aattattggc ggcagctgct
     5401 gaacgccaaa ctgatcacac aacggaagtt cgataatctg actaaggctg aacgaggtgg
     5461 cctgtctgag ttggataaag ccggcttcat caaaaggcag cttgttgaga cacgccagat
     5521 caccaagcac gtggcccaaa ttctcgattc acgcatgaac accaagtacg atgaaaatga
     5581 caaactgatt cgagaggtga aagttattac tctgaagtct aagctggtct cagatttcag
     5641 aaaggacttt cagttttata aggtgagaga gatcaacaat taccaccatg cgcatgatgc
     5701 ctacctgaat gcagtggtag gcactgcact tatcaaaaaa tatcccaagc ttgaatctga
     5761 atttgtttac ggagactata aagtgtacga tgttaggaaa atgatcgcaa agtctgagca
     5821 ggaaataggc aaggccaccg ctaagtactt cttttacagc aatattatga attttttcaa
     5881 gaccgagatt acactggcca atggagagat tcggaagcga ccacttatcg aaacaaacgg
     5941 agaaacagga gaaatcgtgt gggacaaggg tagggatttc gcgacagtcc ggaaggtcct
     6001 gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta cagaccggag gcttctccaa
     6061 ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc gcacgcaaaa aagattggga
     6121 ccccaagaaa tacggcggat tcgattctcc tacagtcgct tacagtgtac tggttgtggc
     6181 caaagtggag aaagggaagt ctaaaaaact caaaagcgtc aaggaactgc tgggcatcac
     6241 aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac tttctcgagg cgaaaggata
     6301 taaagaggtc aaaaaagacc tcatcattaa gcttcccaag tactctctct ttgagcttga
     6361 aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg cagaaaggta acgagctggc
     6421 actgccctct aaatacgtta atttcttgta tctggccagc cactatgaaa agctcaaagg
     6481 gtctcccgaa gataatgagc agaagcagct gttcgtggaa caacacaaac actaccttga
     6541 tgagatcatc gagcaaataa gcgaattctc caaaagagtg atcctcgccg acgctaacct
     6601 cgataaggtg ctttctgctt acaataagca cagggataag cccatcaggg agcaggcaga
     6661 aaacattatc cacttgttta ctctgaccaa cttgggcgcg cctgcagcct tcaagtactt
     6721 cgacaccacc atagacagaa agcggtacac ctctacaaag gaggtcctgg acgccacact
     6781 gattcatcag tcaattacgg ggctctatga aacaagaatc gacctctctc agctcggtgg
     6841 agacagcagg gctgacccca agaagaagag gaaggtgtga aagggttcga tccctaccgg
     6901 ttagtaatga gtttatctag acccagcttt cttgtacaaa gtggtgataa ttcgatatca
     6961 agcttatcga taccgtcgac ctcgagggaa ttccgataat caacctctgg attacaaaat
     7021 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7081 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7141 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7201 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7261 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7321 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7381 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7441 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7501 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7561 gatctccctt tgggccgcct ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa
     7621 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7681 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7741 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7801 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7861 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt
     7921 aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     7981 ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt
     8041 actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg
     8101 aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag
     8161 cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     8221 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     8281 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     8341 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     8401 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat
     8461 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     8521 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     8581 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     8641 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     8701 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8761 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8821 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8881 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8941 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9001 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9061 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     9121 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9181 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9241 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9301 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9361 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9421 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9481 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9541 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9601 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9661 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9721 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9781 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9841 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9901 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9961 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10021 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10081 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10141 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10201 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10261 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10321 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10381 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10441 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10501 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10561 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10621 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10681 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
    10741 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
    10801 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    10861 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    10921 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10981 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    11041 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
    11101 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
    11161 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
    11221 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
    11281 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
    11341 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
    11401 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
    11461 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
    11521 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
    11581 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
    11641 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
    11701 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
    11761 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
    11821 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
    11881 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
    11941 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
    12001 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
    12061 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
    12121 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
    12181 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
    12241 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
    12301 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
    12361 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
    12421 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
    12481 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
    12541 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
    12601 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
    12661 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
    12721 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    12781 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    12841 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    12901 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    12961 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    13021 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    13081 tatttgttaa atatgtacta caaacttagt agt
//
