LOCUS       sequence-175134-       10947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of CUL4B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Han T, Goralski M, Gaskill N, Capota E, Kim J, Ting TC, Xie Y, 
            Williams NS, Nijhawan D
  TITLE     Anticancer sulfonamides target splicing by inducing RBM39 
            degradation via recruitment to DCAF15.
  JOURNAL   Science. 2017 Mar 16. pii: eaal3755. doi: 10.1126/science.aal3755.
  PUBMED    28302793
REFERENCE   2  (bases 1 to 10947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Mar 16. pii: eaal3755. doi: 10.1126/science.aal3755."
COMMENT     Sequence Label: pLVX CUL4B 3xFlag IRES puro
FEATURES             Location/Qualifiers
     source          1..10947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5554..5619
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(5651..6224,6225..6227,6228..6236)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5818..5835)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             6256..6855
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6256..6275)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6752..6772
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6869..7457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6922..6942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7340..7351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7664..8297
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8425..8441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8425..8441)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8438..8460)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8449..8465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8473..8479,8480..8497,8498..8503))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8518..8539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8656..8673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8827..9415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8907..8926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9586..10377,10378..10446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10209..10228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10447..10551)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10599..10733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10636..10655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10690..10709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcat gatgtcacag
     2821 tcatctggat caggagatgg gaatgatgat gaggctacta cctctaaaga cggtggtttt
     2881 tcttccccca gtccctcagc tgctgctgct gctcaggagg tcagatctgc cactgatggt
     2941 aataccagca ccactccgcc cacctctgcc aagaagagaa agttaaacag cagcagcagt
     3001 agcagcagta acagtagtaa cgagagagaa gactttgatt ccacctcttc ctcctcttcc
     3061 actcctcctt tacaacccag ggattcggca tccccttcaa cctcgtcctt ctgcctgggg
     3121 gtttcagtgg ctgcttccag ccacgtaccg atacagaaga agctgcgttt tgaagacacc
     3181 ctggagtttg tagggtttga tgcgaagatg gctgaggaat cctcctcctc ctcctcctca
     3241 tcttcaccaa ctgctgcaac atctcagcag cagcaactta aaaataagag tatattaatc
     3301 tcttctgtgg cttcggtgca tcatgcaaac ggcctagcca aatcttctac caccgtctct
     3361 agctttgcta acagcaaacc tggctctgct aagaagttag tgatcaagaa ctttaaagat
     3421 aagcctaaat taccagaaaa ctacacagat gaaacctggc aaaaactgaa agaagcagtg
     3481 gaagctattc agaatagtac ttcaattaag tacaatttag aagaactcta ccaggctgta
     3541 gaaaatctct gttcttacaa gatttctgca aacttgtaca aacagctgag acagatctgc
     3601 gaagatcaca tcaaagcaca gattcatcaa ttcagagagg attcattgga tagcgttctt
     3661 tttttaaaga agattgatag atgctggcaa aaccattgca gacaaatgat catgatcagg
     3721 agcatttttt tgtttctgga tagaacttac gttcttcaga attcaatgct accctccatt
     3781 tgggacatgg gactggagtt atttagggct catattataa gtgatcagaa agtgcagaat
     3841 aagacaattg atggcattct tctcttgatt gagagggaaa ggaatggtga agcaattgat
     3901 agaagtttac ttcgaagcct tttaagcatg ctgtctgatt tgcaaattta tcaagattct
     3961 tttgaacaac gatttttgga agaaactaac cggctctatg cagctgaagg ccaaaaatta
     4021 atgcaagaaa gagaggttcc tgaatatcta catcatgtta acaaacgtct agaagaagaa
     4081 gcagacagac ttattactta cttagatcag accacccaga agtcattaat tgctactgta
     4141 gaaaaacaac ttctaggtga acacttaaca gcaattcttc agaaaggttt aaataacctc
     4201 cttgatgaaa accgaattca agatttgtct cttctgtatc agctcttcag tagagttcga
     4261 ggtggagttc aggttctttt gcagcagtgg atcgaatata tcaaggcatt tggcagcact
     4321 attgtaatta atcctgaaaa agataaaacc atggttcaag aattgctgga ttttaaagat
     4381 aaggttgacc atataattga tatctgcttt ctgaagaatg agaaatttat caatgccatg
     4441 aaagaagcat ttgaaacgtt cattaacaaa agaccaaata aaccagctga acttatagct
     4501 aagtatgtag attcaaaact tcgtgcaggc aacaaagaag ctacagatga agaacttgag
     4561 aaaatgttgg ataaaattat gatcatattt agatttatct atggcaagga tgtttttgag
     4621 gccttctata agaaagattt agccaagcgc ctgttagtcg gaaagagtgc atctgtagat
     4681 gctgaaaaat caatgctgtc caaacttaaa catgaatgcg gagctgcttt caccagcaaa
     4741 cttgaaggaa tgtttaaaga catggaactt tctaaagaca tcatgattca gttcaaacag
     4801 tatatgcaga atcagaatgt tccgggaaat attgagttaa ctgtgaatat cctgacaatg
     4861 ggctattggc cgacatatgt gcctatggaa gttcatttac caccagagat ggtaaaactt
     4921 caggagattt tcaagacatt ttacctaggc aaacatagtg gcaggaaact tcagtggcag
     4981 tcaaccatag gacactgtgt gttaaaagca gaatttaaag agggtaaaaa ggaactccag
     5041 gtctctcttt ttcaaacact ggtgctgcta atgtttaatg agggagagga gttcagttta
     5101 gaagagatca agcaggcaac tggaatagag gatggagagt taaggagaac actgcagtca
     5161 ttagcctgtg gcaaagctag agttctggcg aaaaatccaa agggcaaaga cattgaagat
     5221 ggtgacaagt tcatttgtaa tgatgatttc aaacataaac ttttcaggat aaagatcaat
     5281 caaatccaga tgaaagaaac ggttgaagaa caagcaagca ctacagaaag agtatttcaa
     5341 gacagacagt atcaaattga tgctgcaatt gttcgaatta tgaagatgag aaagacactt
     5401 agccacaatc tccttgtttc agaagtgtac aaccagttga aatttccagt aaagcctgct
     5461 gatcttaaga agagaataga atctttaatt gaccgggact acatggaaag agataaagaa
     5521 aatccaaacc agtacaacta tattgcaggt agcgactata aagaccacga cggggattac
     5581 aaggaccatg acattgatta caaggacgat gatgataaat agactagttc tagagcggcc
     5641 gcggatcccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     5701 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     5761 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     5821 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     5881 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     5941 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     6001 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc acctcaagcg
     6061 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     6121 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
     6181 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca
     6241 aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6301 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6361 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6421 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6481 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6541 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6601 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6661 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6721 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6781 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6841 aagcccggtg cctagacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     6901 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6961 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7021 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7081 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7141 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7201 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7261 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7321 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7381 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7441 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     7501 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     7561 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     7621 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac
     7681 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7741 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     7801 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     7861 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     7921 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     7981 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     8041 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     8101 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8161 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8221 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8281 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8341 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc
     8401 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8581 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10561 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
    10621 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10681 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    10741 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    10801 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    10861 gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg
    10921 ttaaatatgt actacaaact tagtagt
//
