LOCUS       sequence-17515-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Dimitri CA, Dowdle W, MacKeigan JP, Blenis J, Murphy LO
  TITLE     Spatially separate docking sites on ERK2 regulate distinct signaling
            events in vivo.
  JOURNAL   Curr Biol. 2005 Jul 26. 15(14):1319-24.
  PUBMED    16051177
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2005 Jul 26. 15(14):1319-24."
COMMENT     Sequence Label: pcDNA3-HA-ERK2 D319N
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             108..134
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcttagcgac tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttggta ccacgcgtca tatggcttac ccatacgatg
      121 ttccagatta cgctagcatg gcggcggcgg cggcgggccc ggagatggtc cgcgggcagg
      181 tgttcgacgt ggggccgcgc tacactaatc tctcgtacat cggagaaggc gcctacggca
      241 tggtttgttc tgcttatgat aatctcaaca aagttcgagt tgctatcaag aaaatcagtc
      301 cttttgagca ccagacctac tgtcagagaa ccctgagaga gataaaaatc ctactgcgct
      361 tcagacatga gaacatcatc ggcatcaatg acatcatccg ggcaccaacc attgagcaga
      421 tgaaagatgt atatatagta caggacctca tggagacaga tctttacaag ctcttgaaga
      481 cacagcacct cagcaatgat catatctgct attttcttta tcagatcctg agaggattaa
      541 agtatataca ttcagctaat gttctgcacc gtgacctcaa gccttccaac ctcctgctga
      601 acaccacttg tgatctcaag atctgtgact ttggccttgc ccgtgttgca gatccagacc
      661 atgatcatac agggttcttg acagagtatg tagccacgcg ttggtacaga gctccagaaa
      721 ttatgttgaa ttccaagggt tataccaagt ccattgatat ttggtctgtg ggctgcatcc
      781 tggcagagat gctatccaac aggcctatct tcccaggaaa gcattacctt gaccagctga
      841 atcacatcct gggtattctt ggatctccat cacaggaaga tctgaattgt ataataaatt
      901 taaaagctag aaactatttg ctttctctcc cgcacaaaaa taaggtgccg tggaacagat
      961 tgttcccaaa cg
//
