LOCUS       sequence-175162-        7505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Mauger DM, Lin C, Garcia-Blanco MA
  TITLE     hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth 
            factor receptor 2 exon IIIc.
  JOURNAL   Mol Cell Biol. 2008 Sep . 28(17):5403-19.
  PUBMED    18573884
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Sep . 28(17):5403-19."
COMMENT     Sequence Label: p3x-FLAG-hnRNPF
FEATURES             Location/Qualifiers
     source          1..7505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     79..101
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     93..110
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     94..110
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     805..825
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             884..949
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    2217..2839
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2416..2435)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2863..2883)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2881..3178
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3232..4026
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3286..3305)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3896..3915
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4678..4812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4715..4734)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4769..4788
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4817..4836)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4818..4836)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4850..4866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4850..4866)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4863..4885)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4874..4890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4898..4904,4905..4922,4923..4928))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4943..4964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5081..5098)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5252..5840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5332..5351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6011..6802,6803..6871))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6634..6653
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6872..6976)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7003..7458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7090..7109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7300..7321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
       61 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctga
      121 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
      181 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      241 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctcgttta gtgaaccgtc agaattaacc atggactaca aagaccatga
      901 cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagc ttgcggccgc
      961 gatgatgctg ggccctgagg gaggtgaagg ctttgtggtc aagctccgtg gcctgccctg
     1021 gtcctgctct gttgaggacg tgcagaactt cctctctgac tgcacgattc atgatggggc
     1081 cgcaggtgtc catttcatct acactagaga gggcaggcag agtggtgagg cttttgttga
     1141 acttggatca gaagatgatg taaaaatggc cctgaaaaaa gacagggaaa gcatgggaca
     1201 ccggtacatt gaggtgttca agtcccacag aaccgagatg gattgggtgt tgaagcacag
     1261 tggtcccaac agtgccgaca gcgccaacga tggcttcgtg cggcttcgag gactcccatt
     1321 tggatgcaca aaggaagaaa ttgttcagtt cttctcaggg ttggaaattg tgccaaacgg
     1381 gatcacattg cctgtggacc ccgaaggcaa gattacaggg gaagcgttcg tgcagtttgc
     1441 ctcgcaggag ttagctgaga aggctctagg gaaacacaag gagaggatag ggcacaggta
     1501 cattgaggtg tttaagagca gccaggagga agttaggtca tactcagatc cccctctgaa
     1561 gttcatgtcc gtgcagcggc cagggcccta tgaccggccc gggactgcca ggaggtacat
     1621 tggtatcgtg aagcaggcag gcctggaaag gatgaggcct ggtgcctaca gcacaggcta
     1681 cgggggctac gaggagtaca gtggcctcag tgatggctac ggcttcacca ccgacctgtt
     1741 cgggagagac ctcagctact gtctctccgg aatgtatgac cacagatacg gcgacagtga
     1801 gttcacagtg cagagcacca caggccactg tgtccacatg aggggcctgc cgtacaaagc
     1861 gaccgagaac gacatttaca acttcttctc tcctctcaac cctgtgagag tccatattga
     1921 gattggccca gatggaagag tgacgggtga agcagatgtt gagtttgcta ctcatgaaga
     1981 agctgtggca gctatgtcca aagacagggc caatatgcag cacagatata tagaactctt
     2041 cttgaattca acaacagggg ccagcaatgg ggcgtatagc agccaggtga tgcaaggcat
     2101 gggggtgtct gctgcccagg ccacttacag tggcctggag agccagtcag tgagtggctg
     2161 ttacggggcc ggctacagtg ggcagaacag catgggtggc tatgactagg gatcccgggt
     2221 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
     2281 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
     2341 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
     2401 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
     2461 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
     2521 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
     2581 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
     2641 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
     2701 taaaataact ataccagcag gaggacgtcc agacacagca taggctacct ggccatgccc
     2761 aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc
     2821 agtagatgcc tgttgaattg ggtacgcggc cagcttggct gtggaatgtg tgtcagttag
     2881 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2941 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3001 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3061 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3121 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3181 gaattgatca gcttgggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     3241 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3301 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3361 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     3421 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3481 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     3541 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     3601 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3661 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     3721 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     3781 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     3841 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     3901 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3961 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4021 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     4081 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4141 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccg
     4201 ggctcgatcc cctcgcgagt tggttcagct gctgcctgag gctggacgac ctcgcggagt
     4261 tctaccggca gtgcaaatcc gtcggcatcc aggaaaccag cagcggctat ccgcgcatcc
     4321 atgcccccga actgcaggag tggggaggca cgatggccgc tttggtcgac ccggacggga
     4381 cgctcctgcg cctgatacag aacgaattgc ttgcaggcat ctcatgagtg tgtcttcccg
     4441 ttttccgcct gaggtcactg cgtggatgga gcgctggcgc ctgctgcgcg acggcgagct
     4501 gctcaccacc cactcgccaa gctggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4561 taggctccgc cgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     4621 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     4681 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4741 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4801 catgtctgga tcaattccct atagtgagtc gtattaaatt cgtaatcatg tcatagctgt
     4861 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4921 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4981 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5041 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5881 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5941 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6001 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6061 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6121 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6181 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6241 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6301 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6361 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6421 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6481 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6541 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6601 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6661 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6721 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6781 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6841 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6901 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6961 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     7021 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7081 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7141 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7201 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7261 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7321 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7381 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     7441 tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     7501 ggtgc
//
