LOCUS       sequence-175170-        6808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human cmyc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Ricci MS, Jin Z, Dews M, Yu D, Thomas-Tikhonenko A, Dicker DT, 
            El-Deiry WS
  TITLE     Direct repression of FLIP expression by c-myc is a major determinant
            of TRAIL sensitivity.
  JOURNAL   Mol Cell Biol. 2004 Oct . 24(19):8541-55.
  PUBMED    15367674
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2004 Oct . 24(19):8541-55."
COMMENT     Sequence Label: pcDNA3-cmyc
FEATURES             Location/Qualifiers
     source          1..6808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      132..267
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     194..213
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             348..1142
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(402..421)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1012..1031
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1316..1437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1353..1372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1407..1426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1486..1502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1486..1502)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1499..1521)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1510..1526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1534..1540,1541..1558,1559..1564))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1579..1600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1717..1734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1888..2476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1968..1987)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2647..3438,3439..3507))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3270..3289
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3508..3612)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3687..3706)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3878..4257
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4258..4461
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4412..4432
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4506..4525
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4506..4524
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4607..5926
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     promoter        complement(6004..6022)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6005..6022)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6042..6059)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6048..6272
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6318..6746
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6405..6424)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6615..6636
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6755..6775)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(6760..6808,1..281)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
       61 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
      121 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
      181 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
      241 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
      301 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
      361 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
      421 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
      481 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
      541 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
      601 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
      661 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
      721 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
      781 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
      841 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
      901 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
      961 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     1021 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     1081 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     1141 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     1201 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     1261 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     1321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     1441 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     1501 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1561 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1621 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1681 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1741 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1801 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1861 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1921 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1981 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2041 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2101 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2161 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2221 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2281 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2341 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2401 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2461 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2521 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2581 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2641 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2701 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2761 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2821 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2881 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2941 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3001 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3061 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3121 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3181 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3241 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3301 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3361 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3421 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3481 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3541 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3601 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     3661 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3721 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     3781 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     3841 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     3901 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     3961 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4021 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     4081 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     4141 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     4201 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     4261 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     4321 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     4381 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     4441 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
     4501 gaaattaata cgactcacta tagggagacc caagcttggt accgagctcg gatccactag
     4561 taacggccgc cagtgtgctg gaattcggct tatacgcgga tccaccatgc ccctcaacgt
     4621 tagcttcacc aacaggaact atgacctcga ctacgactcg gtgcagccgt atttctactg
     4681 cgacgaggag gagaacttct accagcagca gcagcagagc gagctgcagc ccccggcgcc
     4741 cagcgaggat atctggaaga aattcgagct gctgcccacc ccgcccctgt cccctagccg
     4801 ccgctccggg ctctgctcgc cctcctacgt tgcggtcaca cccttctccc ttcggggaga
     4861 caacgacggc ggtggcggga gcttctccac ggccgaccag ctggagatgg tgaccgagct
     4921 gctgggagga gacatggtga accagagttt catctgcgac ccggacgacg agaccttcat
     4981 caaaaacatc atcatccagg actgtatgtg gagcggcttc tcggccgccg ccaagctcgt
     5041 ctcagagaag ctggcctcct accaggctgc gcgcaaagac agcggcagcc cgaaccccgc
     5101 ccgcggccac agcgtctgct ccacctccag cttgtacctg caggatctga gcgccgccgc
     5161 ctcagagtgc atcgacccct cggtggtctt cccctaccct ctcaacgaca gcagctcgcc
     5221 caagtcctgc gcctcgcaag actccagcgc cttctctccg tcctcggatt ctctgctctc
     5281 ctcgacggag tcctccccgc agggcagccc cgagcccctg gtgctccatg aggagacacc
     5341 gcccaccacc agcagcgact ctgaggagga acaagaagat gaggaagaaa tcgatgttgt
     5401 ttctgtggaa aagaggcagg ctcctggcaa aaggtcagag tctggatcac cttctgctgg
     5461 aggccacagc aaacctcctc acagcccact ggtcctcaag aggtgccacg tctccacaca
     5521 tcagcacaac tacgcagcgc ctccctccac tcggaaggac tatcctgctg ccaagagggt
     5581 caagttggac agtgtcagag tcctgagaca gatcagcaac aaccgaaaat gcaccagccc
     5641 caggtcctcg gacaccgagg agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca
     5701 gaggaggaac gagctaaaac ggagcttttt tgccctgcgt gaccagatcc cggagttgga
     5761 aaacaatgaa aaggccccca aggtagttat ccttaaaaaa gccacagcat acatcctgtc
     5821 cgtccaagca gaggagcaaa agctcatttc tgaagaggac ttgttgcgga aacgacgaga
     5881 acagttgaaa cacaaacttg aacagctacg gaactcttgt gcgtaaccta ggatacgcaa
     5941 gccgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc
     6001 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag
     6061 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6121 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6181 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6241 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6301 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6361 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6421 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6481 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6541 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6601 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6661 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6721 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     6781 aagtccccag gctccccagc aggcagaa
//
