LOCUS       sequence-175178-        1665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Elastin Like Polypeptide: 
            [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, 
            and a TEV-cleavage site, leaving an N-terminal Sortase Recognition 
            Sequence GGG and a C-terminal Cysteine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Gaub
  TITLE     Gaub Lab ELP Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys
FEATURES             Location/Qualifiers
     source          1..1665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             736..756
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgcaccacc accaccacca cggttctggt ggtgaagaac tgttcgctgg tatcgttccg
       61 gttctgatcg aactggacgg tgacgttcac ggtcacaaat tctctgttcg tggtgaaggt
      121 gaaggtgacg ctgactacgg taaactggaa atcaaattca tctgcaccac cggtaaactg
      181 ccggttccgt ggccgaccct ggttaccacc ctgtgctacg gtatccagtg cttcgctcgt
      241 tacccggaac acatgaaaat gaacgacttc ttcaaatctg ctatgccgga aggttacatc
      301 caggaacgta ccatccagtt ccaggacgac ggtaaataca aaacccgtgg tgaagttaaa
      361 ttcgaaggtg acaccctggt taaccgtatc gaactgaaag gtaaagactt caaagaagac
      421 ggtaacatcc tgggtcacaa actggaatac tctttcaact ctcacaacgt ttacatccgt
      481 ccggacaaag ctaacaacgg tctggaagct aacttcaaaa cccgtcacaa catcgaaggt
      541 ggtggtgttc agctggctga ccactaccag accaacgttc cgctgggtga cggtccggtt
      601 ctgatcccga tcaaccacta cctgtctacc cagaccaaaa tctctaaaga ccgtaacgaa
      661 gctcgtgacc acatggttct gctggaatct ttctctgctt gctgccacac ccacggtatg
      721 gacgaactgt accgtgaaaa cctgtacttc caggggggcg gtgtgccggg agaaggagtc
      781 cctggtgtcg gtgtcccagg cgtgggtgtt ccgggtgtgg gcgttccagg cgtaggcgta
      841 ccgggcgcgg gtgttcctgg tgctggtgtt ccgggcggcg gtgttccggg gggtggcgtt
      901 ccgggtgaag gagtaccagg cgaaggcgtg ccgggtgtcg gtgtcccagg cgtgggtgtt
      961 ccgggtgtgg gcgttccagg cgtaggcgta ccgggcgcgg gtgttcctgg tgctggtgtt
     1021 ccgggcggcg gtgttccggg gggtggcgtt ccgggtgagg gtgtaccagg cgaaggggtg
     1081 ccgggtgtcg gtgtcccagg cgtgggtgtt ccgggtgtgg gcgttccagg cgtaggcgta
     1141 ccgggcgcgg gtgttcctgg tgctggtgtt ccgggcggcg gtgttccggg gggtggcgtt
     1201 ccgggtgagg gtgtgccggg agaaggagtc cctggtgtcg gtgtcccagg cgtgggtgtt
     1261 ccgggtgtgg gcgttccagg cgtaggcgta ccgggcgcgg gtgttcctgg tgctggtgtt
     1321 ccgggcggcg gtgttccggg gggtggcgtt ccgggtgaag gagtaccagg cgaaggcgtg
     1381 ccgggtgtcg gtgtcccagg cgtgggtgtt ccgggtgtgg gcgttccagg cgtaggcgta
     1441 ccgggcgcgg gtgttcctgg tgctggtgtt ccgggcggcg gtgttccggg gggtggcgtt
     1501 ccgggtgagg gtgtaccagg cgaaggggtg ccgggtgtcg gtgtcccagg cgtgggtgtt
     1561 ccgggtgtgg gcgttccagg cgtaggcgta ccgggcgcgg gtgttcctgg tgctggtgtt
     1621 ccgggcggcg gtgttccggg gggtggcgtt ccgggtgaag gttgc
//
