LOCUS       sequence-175180-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Elastin Like Polypeptide: 
            [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal 
            TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase 
            Recognition Sequence GGG and a C-terminal Cysteine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Gaub
  TITLE     Gaub Lab ELP Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             745..765
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggtaccc accaccacca ccaccaccca tggtctggtg gtgaagaact gttcgctggt
       61 atcgttccgg ttctgatcga actggacggt gacgttcacg gtcacaaatt ctctgttcgt
      121 ggtgaaggtg aaggtgacgc tgactacggt aaactggaaa tcaaattcat ctgcaccacc
      181 ggtaaactgc cggttccgtg gccgaccctg gttaccaccc tgtgctacgg tatccagtgc
      241 ttcgctcgtt acccggaaca catgaaaatg aacgacttct tcaaatctgc tatgccggaa
      301 ggttacatcc aggaacgtac catccagttc caggacgacg gtaaatacaa aacccgtggt
      361 gaagttaaat tcgaaggtga caccctggtt aaccgtatcg aactgaaagg taaagacttc
      421 aaagaagacg gtaacatcct gggtcacaaa ctggaatact ctttcaactc tcacaacgtt
      481 tacatccgtc cggacaaagc taacaacggt ctggaagcta acttcaaaac ccgtcacaac
      541 atcgaaggtg gtggtgttca gctggctgac cactaccaga ccaacgttcc gctgggtgac
      601 ggtccggttc tgatcccgat caaccactac ctgtctaccc agaccaaaat ctctaaagac
      661 cgtaacgaag ctcgtgacca catggttctg ctggaatctt tctctgcttg ctgccacacc
      721 cacggtatgg acgaactgta ccgtgaaaac ctgtacttcc aggggggcgg tgtgccggga
      781 gaaggagtcc ctggtgtcgg tgtcccaggc gtgggtgttc cgggtgtggg cgttccaggc
      841 gtaggcgtac cgggcgcggg tgttcctggt gctggtgttc cgggcggcgg tgttccgggg
      901 ggtggcgttc cgggtgaagg agtaccaggc gaaggcgtgc cgggtgtcgg tgtcccaggc
      961 gtgggtgttc cgggtgtggg cgttccaggc gtaggcgtac cgggcgcggg tgttcctggt
     1021 gctggtgttc cgggcggcgg tgttccgggg ggtggcgttc cgggtgaagg ttgc
//
