LOCUS       sequence-175191-       12083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for Gal4 inducible human full-length TRAIL with 
            mCherry reporter and constitutive tBFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, 
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs 
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi: 
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
  PUBMED    27693353
REFERENCE   2  (bases 1 to 12083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_tBFP
FEATURES             Location/Qualifiers
     source          1..12083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    865..1438
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1032..1049)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1259..1278
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1457..2167
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1602..1620)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1870..1889
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2252..2739
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2287..2310)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2672..2691
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2760..3461
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(3550..3570)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3968..3979)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4148..4381
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4406..4424)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4407..4424)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4714..4733)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4947..4966)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5066..5088
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5126..5144)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5212..5316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5317..5385,5386..6177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5535..5554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6348..6936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6837..6856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7090..7107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7177..7197)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7182..7511
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7362..7497
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8645..8710
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8840..8860
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9285..9419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9322..9341)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9376..9395
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9587..10220
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10267..10392
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10889..11122
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11307..11351
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11636..11753
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     11979..12003
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggcgatga tggaggtgca gggaggcccg agtctgggac aaacttgcgt cctgatcgtg
       61 atctttactg tcttgctgca gagcctttgc gtagcggtca catatgtgta tttcactaat
      121 gaactgaagc agatgcagga taaatattct aagtctggaa tcgcttgctt cttgaaggag
      181 gacgacagtt attgggatcc caatgacgag gagtccatga acagcccctg ctggcaggtg
      241 aagtggcagt tgcggcaact ggtgcggaag atgatcctgc ggacaagcga ggaaacgatt
      301 tctacagtgc aggaaaagca acagaacatc agcccactgg tgagagagag gggaccccag
      361 cgggtcgccg cacacattac cggaacccgc gggcgctcta ataccctgtc aagtcccaac
      421 tccaagaacg agaaagccct cggccgcaag ataaattcct gggaatcaag ccgcagcggg
      481 cacagttttc tgtcaaactt gcacttgcgc aatggggagt tggttatcca tgagaagggc
      541 ttctattaca tctactctca aacctacttt aggtttcagg aagaaataaa agagaataca
      601 aaaaacgaca aacagatggt gcagtacatc tacaagtata ccagttatcc cgaccctatc
      661 cttctcatga agtcagccag gaactcatgc tggtccaaag acgctgagta tggactgtac
      721 agcatctacc agggcgggat ctttgagctg aaggaaaatg accgcatctt tgtatcagtg
      781 actaatgagc atctcattga tatggatcac gaagcttcat tttttggagc ctttctcgtc
      841 ggctaagatc cttgacttgc ggcccccctc tccctccccc ccccctaacg ttactggccg
      901 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
      961 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     1021 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     1081 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     1141 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     1201 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     1261 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     1321 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     1381 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataaat
     1441 gagcgctggc gggtccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     1501 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     1561 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1621 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     1681 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     1741 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     1801 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     1861 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     1921 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     1981 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     2041 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     2101 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     2161 caagtagggc cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga
     2221 gtcgacctgc acgaggttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag
     2281 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     2341 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     2401 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     2461 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     2521 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     2581 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     2641 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     2701 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     2761 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
     2821 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     2881 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
     2941 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
     3001 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     3061 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     3121 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     3181 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
     3241 ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca
     3301 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
     3361 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
     3421 actgcgacct ccctagcaaa ctggggcaca agcttaatta atgcaggcat gcaagcttga
     3481 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3541 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3601 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3661 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3721 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3781 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3841 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3901 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3961 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4021 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcgtc
     4081 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4141 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     4201 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4261 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4321 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4381 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     4441 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     4501 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     4561 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     4621 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     4681 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     4741 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     4801 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     4861 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     4921 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4981 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     5041 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5101 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5161 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5221 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5281 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5341 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5401 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5461 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5521 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5581 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5641 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5701 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5761 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5821 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5881 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5941 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6001 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6061 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6121 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6181 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6241 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6301 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6361 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6421 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6481 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6541 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6601 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6661 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6721 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6781 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6841 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6901 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6961 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7141 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
     7201 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7261 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7321 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7381 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7441 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7501 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
     7561 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
     7621 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
     7681 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
     7741 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
     7801 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
     7861 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     7921 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     7981 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     8041 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     8101 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     8161 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     8221 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     8281 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     8341 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
     8401 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
     8461 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
     8521 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
     8581 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     8641 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     8701 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     8761 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     8821 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     8881 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     8941 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     9001 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     9061 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     9121 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     9181 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     9241 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     9301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     9421 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     9481 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     9541 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     9601 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9661 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     9721 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     9781 accagcttgt tacaccctgt gagcctgcat gggatggatg acccgtagag agaagtgtta
     9841 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     9901 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     9961 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    10021 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10081 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10141 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10201 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10261 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10321 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    10381 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10441 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10501 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    10561 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10621 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10681 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
    10741 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    10801 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    10861 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    10921 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    10981 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    11041 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    11101 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    11161 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    11221 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    11281 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    11341 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    11401 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    11461 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    11521 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    11581 ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta
    11641 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    11701 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    11761 attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcggagcac
    11821 tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct ccgaacgtcg
    11881 gagcactgtc ctccgaacgg agcatgtcct ccgaacgtcg gagcactgtc ctccgaacga
    11941 ctagttaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
    12001 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
    12061 agcctctcga cattcgttgg atc
//
