LOCUS       sequence-175200-       12269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and
            constitutive mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12269)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, 
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs 
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi: 
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
  PUBMED    27693353
REFERENCE   2  (bases 1 to 12269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_
            Pemb...
FEATURES             Location/Qualifiers
     source          1..12269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             58..87
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1453..1506
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1564..1590
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2370..2869
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2405..2428)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2802..2821
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2890..3600
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3035..3053)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3303..3322
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3677..4265
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3730..3750)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4148..4159)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4328..4561
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4586..4604)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4587..4604)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4894..4913)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5127..5146)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5246..5268
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5306..5324)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5392..5496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5497..5565,5566..6357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5715..5734)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6528..7116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7017..7036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7270..7287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7357..7377)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7362..7691
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7542..7677
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8825..8890
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9020..9040
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9465..9599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9502..9521)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9556..9575
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9767..10400
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10447..10572
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11069..11302
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11487..11531
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11816..11933
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     12159..12183
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      join(12264..12269,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggattgga catggagagt gttttgtctg cttgcggtga cacctggcgc tcatcctgag
       61 cagaagctga tcagcgagga ggacctgcag gtacaactgg tccagtcagg cgtcgaggtt
      121 aaaaagcccg gtgcctcagt gaaggtctcc tgcaaagcca gtggttacac cttcactaat
      181 tactatatgt attgggtcag acaggcaccc ggccagggtc ttgaatggat gggaggcatc
      241 aatcccagca atggagggac aaacttcaat gaaaagttca aaaatcgcgt gaccctcaca
      301 accgactcct ccacaacaac agcatatatg gaactgaagt ctcttcagtt tgatgacacc
      361 gccgtctact actgcgcccg acgagactac cggttcgata tgggattcga ctattggggt
      421 caggggacga cagtgacagt ctcctccgca tctacaaagg gaccatcagt gtttcctctg
      481 gccccgtgct cccgctctac cagcgaatct accgcagcat tgggctgttt ggtgaaggac
      541 tacttccctg agccagtgac cgtgtcctgg aacagcgggg cactcactag cggagtgcac
      601 acatttccag ccgtgcttca aagcagcggt ctctattccc tctccagcgt ggtgactgtg
      661 ccatccagct ctctcggcac gaagacctat acctgcaacg tggaccacaa gccttcaaat
      721 accaaggtgg ataaacgcgt cgaatccaaa tatggcccac cctgcccgcc atgtccagct
      781 ccagagtttc tcggcgggcc tagcgtgttc ttgtttcccc ccaaacccaa ggacacgctc
      841 atgatttcca gaacacctga agttacctgc gtcgtcgtag acgtcagtca ggaggacccg
      901 gaagttcagt ttaactggta cgtggacggt gttgaggtgc ataatgccaa aaccaagcca
      961 agagaagagc agttcaattc tacatatcga gtggtgtcag tcctgacagt tctccaccag
     1021 gactggttga acggcaaaga gtacaaatgc aaggtctcca acaaaggatt gccgagcagt
     1081 atagagaaaa caattagtaa agctaagggt cagcccagag agccccaggt gtacacgttg
     1141 ccaccttcac aggaagagat gacgaagaac caagtgtctc tgacctgcct ggtgaagggc
     1201 ttctacccat cagacattgc agtggagtgg gaaagcaatg ggcagcctga gaacaactac
     1261 aaaactacac cccccgtcct ggacagcgat ggcagtttct tcctgtactc aaggttgact
     1321 gtggacaaaa gcagatggca ggaaggcaat gtgttcagct gtagtgtgat gcacgaggcc
     1381 ctccacaatc attacactca gaaatccctc tctctgagcc tgggaaagag gaagcgacgg
     1441 agtgggtcag gcgaggggag aggctcactc ctcacgtgtg gagacgttga agaaaacccc
     1501 ggaccaatgg cttggagtcc tctgttcctg accctgatta ctcactgcgc tggaagctgg
     1561 gcttacccat acgatgttcc agattacgct gagattgttc tgacccaatc tcctgcaaca
     1621 ctgagcctgt cccccggaga gagggcaacc ctctcctgcc gcgccagtaa gggagtgtct
     1681 acgtctggat attcctatct ccattggtat cagcagaagc ctggccaggc gcccaggctt
     1741 ctcatctacc ttgcttccta tctggagtcc ggggtgccag cccgcttttc tgggtccggc
     1801 tctggtaccg acttcaccct gacaatttcc tcactggagc ctgaggattt cgctgtctac
     1861 tattgtcaac attccagaga ccttcccctg acctttggag ggggtacaaa agtggagata
     1921 aaacgaacag tggccgcccc atctgtattc atctttcctc catctgatga acagcttaag
     1981 tccggcactg cttctgttgt ttgcctgctt aacaattttt acccgcggga agccaaggtg
     2041 caatggaaag tggacaacgc cctgcagtct ggtaacagtc aggaaagcgt gactgaacag
     2101 gacagcaaag actccacata ctccttgagt tccaccttga cgctcagtaa ggcagactac
     2161 gagaagcaca aggtttatgc ctgtgaagtg actcaccagg gcctctctag cccagtgact
     2221 aagtccttca accgcgggga gtgttgagat ccttgacttg cggccgcaac tcccacctgc
     2281 aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcatctag gcgccggaat
     2341 tagatctctc gaggttaacg aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     2401 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     2461 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     2521 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     2581 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     2641 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     2701 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     2761 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     2821 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     2881 aagcttacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     2941 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     3001 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     3061 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     3121 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     3181 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     3241 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     3301 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     3361 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     3421 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     3481 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     3541 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     3601 agcggccgcg actctagagt cgacctgcag gcatgcaagc ttgcaggcat gcaagcttga
     3661 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3721 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3781 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3841 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3901 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3961 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4021 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4081 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4141 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4201 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4261 cccgcatcga taccgtcgtc cacggcagct gtagatctta gccacttttt aaaagaaaag
     4321 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     4381 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4441 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4501 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4561 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     4621 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     4681 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     4741 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     4801 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     4861 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     4921 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     4981 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     5041 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     5101 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5161 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     5221 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5281 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5341 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5401 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5461 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5521 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5581 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5641 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5701 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5761 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5821 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5881 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5941 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6001 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6061 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6121 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6181 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6241 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6301 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6361 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6421 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6481 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6541 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6601 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6661 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6721 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6781 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6841 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6901 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6961 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7021 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7081 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7141 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7201 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7261 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7321 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
     7381 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7441 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7501 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7561 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7621 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7681 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
     7741 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
     7801 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
     7861 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
     7921 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
     7981 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
     8041 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     8101 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     8161 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     8221 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     8281 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     8341 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     8401 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     8461 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     8521 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
     8581 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
     8641 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
     8701 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
     8761 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     8821 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     8881 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     8941 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     9001 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     9061 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     9121 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     9181 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     9241 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     9301 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     9361 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     9421 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     9481 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9541 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     9601 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     9661 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     9721 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     9781 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9841 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     9901 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     9961 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    10021 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    10081 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
    10141 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    10201 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10261 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10321 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10381 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10441 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10501 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    10561 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10621 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10681 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    10741 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10801 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10861 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
    10921 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    10981 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    11041 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    11101 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    11161 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    11221 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    11281 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    11341 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    11401 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    11461 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    11521 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    11581 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    11641 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    11701 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    11761 ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta
    11821 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    11881 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    11941 attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcggagcac
    12001 tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct ccgaacgtcg
    12061 gagcactgtc ctccgaacgg agcatgtcct ccgaacgtcg gagcactgtc ctccgaacga
    12121 ctagttaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
    12181 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
    12241 agcctctcga cattcgttgg atcgccgcc
//
