LOCUS       sequence-175201-       10277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from
            C-terminal of LTCCs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Liu N, Yang Y, Ge L, Liu M, Colecraft HM, Liu X
  TITLE     Cooperative and acute inhibitionby multiple C-terminal motifs of 
            L-type Ca2+ channels.
  JOURNAL   Elife. 2017 Jan 6;6. pii: e21989. doi: 10.7554/eLife.21989.
  PUBMED    28059704
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-01-6"; volume: "6. pii: e21989. doi"; pages: " 
            10.7554/eLife.21989"
COMMENT     Sequence Label: alpha1Ddelta-G12-FRB
FEATURES             Location/Qualifiers
     source          1..10277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5600..5878
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             5904..5945
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5955..5972
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5995..6012)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6001..6225
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6271..6699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6358..6377)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6568..6589
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6708..6728)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6713..7042
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6893..7028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6955..6974
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7090..7137
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7156..7554
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7712..7833
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7749..7768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7803..7822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7882..7898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7882..7898)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7895..7917)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7906..7922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7930..7936,7937..7954,7955..7960))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7975..7996
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8113..8130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8284..8872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8364..8383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9043..9834,9835..9903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9666..9685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9904..10008)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10087..10106)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagcta
      661 gctgttcgtg gaaatgcagc atcaacggca gcagcaagag gaccacgcga acgaggcaaa
      721 ctatgcaaga ggcaccagac tccctatttc tggtgaagga ccaacttctc agccgaacag
      781 ctccaagcaa accgtcctgt cttggcaagc tgcaattgat gctgctagac aggccaaggc
      841 cgcccagacc atgagcacgt ctgcaccccc acctgtagga tctctctccc aaagaaaacg
      901 tcagcaatac gccaagagca aaaaacaggg taactcgtct aacagccgac ctgcccgtgc
      961 cctcttctgt ttatccctca ataaccccat ccgaagagcc tgcattagta tagtggattg
     1021 gaaaccattt gacatattta tattattggc tatttttgcc aattgtgtgg ccttagctat
     1081 ttacatcccg ttccctgaag atgattctaa ttcaacaaat cataacttgg aaaaagtaga
     1141 atatgccttc ctgattatct ttacagtgga gacatttctg aagattatag cgtacggact
     1201 gctgctgcat cctaatgctt acgttaggaa cggatggaac ttactggatt ttgttatagt
     1261 aatagtagga ttgtttagtg taattttgga acaattaacc aaagaaacag aaggcgggaa
     1321 ccactctagt ggcaaatcag gaggctttga tgtcaaagcc ctccgtgcct tccgagtgtt
     1381 aagaccgctt cgacttgtat catcagtgcc cagtttacaa gttgttctga actccattat
     1441 aaaagccatg gtccccctcc tccacatagc ccttttggtg ttattcgtaa ttatcatcta
     1501 tgcgattata gggttggaac tttttattgg aaaaatgcac aaaacctgtt tttttgctga
     1561 ctcagatata gtagctgaag aggacccagc tccatgcgcg ttctcaggga atggacgcca
     1621 gtgtgccgcc aatggcacgg aatgtaggag cggctgggtc ggccccaatg gaggcatcac
     1681 taattttgac aactttgcct ttgccatgct cactgtgttc cagtgcatca ccatggaggg
     1741 ctggacagat gtgctctact gggttaatga tgcgatagga tgggagtggc catgggtgta
     1801 ttttgttagt ctgatcatcc ttggctcatt tttcgtcctt aacctggttc ttggtgtcct
     1861 tagtggagaa ttctcaaagg aaagagagaa ggctaaagca cgtggagatt tccagaagct
     1921 tcgggagaag cagcagctgg aagaggatct caagggctac ttggactgga tcacccaggc
     1981 agaagacata gatcctgaga atgaagagga gggtggagag gagggcaaac gaaacactag
     2041 catgcccacc agtgagaccg aatctgtgaa cacagagaac gtaagtggtg aaggcgagac
     2101 ccagggatgc tgtggaagtc tctggtgctg gtggaaaaga agaggcgcgg ccaagactgg
     2161 gccctctggg tgtcggcggt ggggccaagc catctcgaaa tccaagctca gccgtcggtg
     2221 gcgtcgctgg aaccggttca atcgcaggag atgcagggca gctgtgaagt ctgtcacgtt
     2281 ttactggctg gtgatcgttc tggtgtttct caacactctg acgatttcct ctgagcacta
     2341 caatcagcca gactggctga cgcagattca agatatagcg aacaaagtgc tcttggcttt
     2401 gttcacctgt gagatgctgg tgaagatgta cagcctgggc ctccaggcct acttcgtctc
     2461 tctcttcaac cgcttcgatt gctttgtggt gtgtggggga atcaccgaaa ccatcctggt
     2521 ggaactggag ctcatgtctc ccctgggcgt ctctgtgttc cggtgtgtac gtctcctgag
     2581 gatcttcaaa gtgaccaggc actggacttc cctgagcaac ttggtggcgt ccctgttaaa
     2641 ctccatgaag tccatcgcct ctctgctgtt gctgctcttc ctcttcatca tcatcttctc
     2701 cctgctgggg atgcagctgt tcggagggaa gttcaatttc gatgagacac agaccaagcg
     2761 aagcaccttc gacaacttcc cacaggcgct cctgactgtg ttccagatcc tgacgggcga
     2821 agactggaat gcggtgatgt atgacggcat catggcctac ggaggcccgt cctcctctgg
     2881 aatgattgtc tgtatttact tcatcatcct tttcatctgt ggtaactata tcctgctgaa
     2941 cgtcttcttg gccattgctg tagacaattt ggctgatgct gaaagtctga acactgctca
     3001 gaaagaggaa gccgaagaaa aagaaaggaa aaaaattgcc agaaaagaaa gcctagaaaa
     3061 caaaaaaaac aacaaaccag aggtcaacca gatagccaac agtgacaaca aggttactat
     3121 tgatgactat caagaggagg ctgaagataa ggacccttat ccaccctgcg atgtgccagt
     3181 aggtgaagag gaagaggagg aggaagagga cgagcctgag gttcctgctg gtccccgtcc
     3241 tcgaagaatc tcagagttga acatgaagga gaaaattgcg cccatccccg aaggaagcgc
     3301 attcttcatt cttagcaaga ccaacccgat ccgtgtgggt tgccacaaac tcatcaacca
     3361 ccacatcttc accaacctca tcctggtctt catcatgctg agcagcgctg ccctggccgc
     3421 agaggatccc atccgcagcc actccttccg caacactata ctgggctact ttgactatgc
     3481 tttcacagcc atctttacgg ttgaaatcct gttaaagatg acaacttttg gagcgttctt
     3541 gcacaaaggg gctttctgta ggaactactt caatttgctg gacatgctgg tcgttggggt
     3601 gtctctggtg tcatttggga ttcaatccag tgccatctcg gttgtgaaga ttctgagggt
     3661 cttaagggtc ttgaggcctc tcagagcaat caacagagca aaaggactta agcacgtggt
     3721 ccagtgtgtc tttgtggcca tccgaaccat cggcaacatc atgatcgtca cgaccctgct
     3781 ccagttcatg tttgcttgca ttggggtcca gctgttcaag gggaagttct accgttgcac
     3841 agatgaggcc aaaagtaacc ccgaggagtg cagggggctt ttcatccttt ataaggacgg
     3901 cgatgtcgac agtcccgtgg tccgtgagag gatctggcaa aacagtgatt tcaatttcga
     3961 caatgtcctt tcggctatga tggtgctctt cacggtctcg acttttgagg gctggcccgc
     4021 gttgctgtac aaagctattg actcaaacgg agagaacgtt ggtcctgtct acaactaccg
     4081 tgtggagatc tccatcttct tcattatcta catcatcatc gtggccttct tcatgatgaa
     4141 tatcttcgtg ggcttcgtca tcgtcacctt ccaggagcag ggagaaaagg agtataagaa
     4201 ctgtgagctg gacaaaaatc agcgtcagtg tgtggaatat gccttgaagg cccgcccctt
     4261 aaggagatac atccccaaaa acccatacca gtacaagttc tggtacgtgg tgaactcctc
     4321 gcctttcgaa tatatgatgt ttgtcctcat catgctcaac acgctctgcc tggccatgca
     4381 gcactatgag caatccaaga tgttcaatga cgccatggac attctgaaca tggtcttcac
     4441 gggggtcttc accgttgaga tggttttgaa agtcatcgca tttaagccca agcactattt
     4501 cactgatgca tggaatactt ttgatgcctt aattgttgtc ggtagcgtcg ttgatattgc
     4561 tataactgaa gtgaataact ctgaagagag caatagaatc tccatcacct ttttccgtct
     4621 tttccgagtg atgcggttgg tgaagcttct cagcagaggg gaaggcatcc ggactctgct
     4681 atggaccttc attaagtcct tccaggcact cccatatgtc gccctcctca ttgccatgct
     4741 cttcttcatc tacgccgtca ttggcatgca gatgtttggg aaggttgcca tgagagataa
     4801 caaccagatc aataggaaca acaacttcca gacgtttccc caggcagtgc tgctgctctt
     4861 caggtgtgca acaggggagg cctggcagga gatcatgctc gcctgcctcc ctgggaagct
     4921 gtgtgacccg gactcagatt acaacccagg agaggaatat acttgtggga gcaactttgc
     4981 cattgtctac ttcatcagct tttacatgct ctgtgcgttc ctgatcatca acctcttcgt
     5041 ggctgtcatc atggacaatt ttgactatct gacgagggac tggtctattc tggggcctca
     5101 ccacttggac gaattcaaaa gaatatggtc tgaatatgac cccgaggcaa agggaaggat
     5161 aaaacacctt gatgtggtca ctttgctccg acgtatccag cctcccctgg gatttggaaa
     5221 gttgtgtcca caccgagtgg cgtgtaagag attggtcgcc atgaacatgc ctctcaacag
     5281 tgatgggaca gtcatgttca atgcaactct gtttgctttg gtacggacgg ctctcaagat
     5341 caagactgaa ggcaacctgg agcaagctaa tgaagagctc cgcgctgtga taaagaaaat
     5401 ctggaagaag acaagcatga agctacttga ccaagttgtc cctccagctg gtgatgatga
     5461 ggtaaccgtg gggaagttct atgctacttt cctgatacag gactacttta ggaaattcaa
     5521 gaagcggaaa gagcaaggcc tggtggggtc tagtggaggt ggaggtggtg gcggtggtgg
     5581 aggaggagga gcggccgcta tcctctggca tgagatgtgg catgaaggcc tggaagaggc
     5641 atctcgtttg tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc tggagccctt
     5701 gcatgctatg atggaacggg gcccccagac tctgaaggaa acatccttta atcaggccta
     5761 tggtcgagat ttaatggagg cccaagagtg gtgcaggaag tacatgaaat cagggaatgt
     5821 caaggacctc ctccaagcct gggacctcta ttatcatgtg ttccgacgaa tctcaaagta
     5881 atctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
     5941 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
     6001 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6061 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     6121 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     6181 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     6241 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     6301 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6361 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6421 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6481 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6541 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6601 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6661 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6721 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6781 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6841 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6901 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6961 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     7021 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     7081 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     7141 gtgaggaact aaaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga
     7201 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
     7261 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
     7321 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
     7381 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
     7441 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
     7501 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcactt
     7561 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     7621 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7681 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7741 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7801 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     7861 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7921 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7981 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8041 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8101 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8161 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8221 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8281 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8341 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8401 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8461 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8521 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8581 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8641 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8701 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8761 taccttcgga aaaagagttg gtagctcttg atccggcaaa aaaaccaccg ctggtagcgg
     8821 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8881 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8941 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9001 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9061 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9121 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9181 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9241 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9301 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9361 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9421 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9481 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9541 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9601 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9661 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9721 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9781 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9841 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9901 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9961 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10021 aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc tatggtgcac
    10081 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    10141 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
    10201 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
    10261 ggccagatat acgcgtt
//
