LOCUS       sequence-175202-       11957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for Gal4 inducible leucinezipper TRAIL with 
            mCherry reporter and constitutive tBFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, 
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs 
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi: 
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
  PUBMED    27693353
REFERENCE   2  (bases 1 to 11957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_human_leucinezipperTRAIL
            _IRES_mCh...
FEATURES             Location/Qualifiers
     source          1..11957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    730..1303
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(897..914)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1124..1143
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1322..2032
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1467..1485)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1735..1754
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2117..2604
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2152..2175)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2537..2556
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2625..3326
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(3415..3435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3833..3844)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4013..4246
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4271..4289)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4272..4289)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4579..4598)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4812..4831)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4931..4953
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4991..5009)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5077..5181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5182..5250,5251..6042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5400..5419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6213..6801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6702..6721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6955..6972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7042..7062)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7047..7376
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7227..7362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8510..8575
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8705..8725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9150..9284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9187..9206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9241..9260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9452..10085
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10132..10257
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10754..10987
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11172..11216
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11501..11618
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     11844..11868
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgggcgtta aagtcctttt cgccctgatc tgcattgccg tcgcagaggc catgaagcag
       61 atagaagata agattgaaga gatccttagc aaaatttatc atattgagaa cgaaattgcc
      121 cggatcaaaa agctgatcgg cgaaagaacc tctgaagaaa ccatctcaac cgttcaggag
      181 aaacaacaaa acatatcacc tctggtccgg gaacgcggac ctcagcgcgt cgccgcgcat
      241 atcaccggaa cccgcggaag atcaaataca ctgtccagcc caaacagcaa gaacgagaaa
      301 gcactcggcc ggaagatcaa ttcatgggag agctcaagga gcggccactc tttcctcagc
      361 aacttgcatc ttcggaacgg cgaactggtg atccatgaga agggcttcta ttatatatac
      421 tctcagacat acttccggtt ccaggaagag atcaaagaga atactaagaa cgataaacaa
      481 atggttcaat atatatacaa gtatacatca taccctgatc caatcctgct catgaagtcc
      541 gcccgcaaca gctgttggag taaagacgca gaatacggct tgtactccat ctaccagggg
      601 ggaatttttg agctgaaaga aaatgatcgc atcttcgtca gcgtgaccaa tgagcacctg
      661 attgacatgg atcatgaggc cagtttcttc ggtgccttct tggttggctg agatccttga
      721 cttgcggccc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
      781 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
      841 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
      901 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
      961 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     1021 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     1081 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     1141 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     1201 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     1261 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taaatgagcg ctggcgggtc
     1321 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     1381 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1441 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     1501 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     1561 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     1621 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     1681 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     1741 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     1801 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     1861 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     1921 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     1981 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt agggccgcaa
     2041 ctcccacctg caacatgcgt gactgactga ggccgcgact ctagagtcga cctgcacgag
     2101 gttaacgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     2161 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     2221 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     2281 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     2341 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     2401 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     2461 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcggggcggg cgcccgaagg
     2521 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     2581 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgagc gagctgatta
     2641 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
     2701 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     2761 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
     2821 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
     2881 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
     2941 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     3001 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
     3061 cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg
     3121 gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga
     3181 acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca
     3241 acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta
     3301 gcaaactggg gcacaagctt aattaatgca ggcatgcaag cttgatatca agcttatcga
     3361 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3421 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3481 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3541 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3601 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3661 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3721 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3781 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3841 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3901 tcgccttcgc cctcagacga gtcggatctc cctttgggcc cggtaccttt aagaccaatg
     3961 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     4021 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     4081 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4141 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4201 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
     4261 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     4321 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     4381 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     4441 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
     4501 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     4561 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     4621 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     4681 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     4741 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
     4801 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     4861 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     4921 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4981 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5041 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5101 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5161 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5221 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5281 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5341 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5401 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5461 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5521 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5581 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5641 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5701 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5761 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5821 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5881 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5941 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6001 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6061 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6121 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6181 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6241 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6301 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6361 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6421 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6481 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6541 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6601 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6661 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6721 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6781 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6841 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6901 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6961 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     7021 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     7081 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7201 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7261 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7321 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7381 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
     7441 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     7501 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     7561 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     7621 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     7681 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
     7741 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     7801 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     7861 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     7921 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
     7981 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     8041 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
     8101 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     8161 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
     8221 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
     8281 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
     8341 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
     8401 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
     8461 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     8521 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     8581 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     8641 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     8701 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     8761 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     8821 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     8881 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     8941 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     9001 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
     9061 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     9121 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     9181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     9301 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
     9361 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
     9421 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
     9481 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     9541 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     9601 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     9661 cctgtgagcc tgcatgggat ggatgacccg tagagagaag tgttagagtg gaggtttgac
     9721 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     9781 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     9841 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     9901 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9961 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    10021 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    10081 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    10141 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    10201 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    10261 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    10321 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    10381 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    10441 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    10501 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    10561 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
    10621 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    10681 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    10741 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    10801 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10861 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10921 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10981 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    11041 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    11101 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    11161 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    11221 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    11281 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    11341 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
    11401 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
    11461 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    11521 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    11581 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    11641 gagatccagt ttggatcgat aagcttgata tcgaattcgg agcactgtcc tccgaacgtc
    11701 ggagcactgt cctccgaacg tcggagcact gtcctccgaa cgtcggagca ctgtcctccg
    11761 aacggagcat gtcctccgaa cgtcggagca ctgtcctccg aacgactagt taggcgtgta
    11821 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
    11881 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ctcgacattc
    11941 gttggatcgc cgccacc
//
