LOCUS       sequence-175215-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Ubl4A tagged with VENUS at C terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pVENUS-N1-Ubl4A
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      584..593
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(635..656)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttcg aattctgcag tcgacggtac cgcggccatg cagctgacgg tgaaggcgct
      121 gcagggccgc gagtgcagcc tgcaggtgcc agaggacgag ctggtgtcca cgctgaagca
      181 gctggtctcc gagaagctga acgtcccagt gcgccagcag cggctgctgt tcaagggcaa
      241 ggccctggca gatgggaaac gactctcgga ttatagcatc gggcccaact ccaagctcaa
      301 cctagtggtc aaacccctgg agaaggtgct actagaagaa ggcgaggccc agaggctggc
      361 cgactcccca cccccgcagg tctggcagct gatctccaaa gtcttggccc gccacttcag
      421 tgcggcagat gccagcaggg tcctggaaca gctacagagg gattacgaga ggtccctgag
      481 tcgcctgacg ctggacgaca tcgaacggtt ggccagccgc ttcctgcacc ctgaagtgac
      541 tgagacaatg gagaagggct tctccaaacg ggatccaccg gtcgccacca tggtgagcaa
      601 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      661 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      721 cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      781 cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      841 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggac
//
