LOCUS       sequence-175224-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express FNTA with a Venus tag at C terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pVENUS-N1-FNTA
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttac cgccatggcg gccaccgagg gggtcgggga ggctgcgcaa gggggcgagc
      121 ccgggcagcc ggcgcaaccc ccgccccagc cgcacccacc gccgccccag cagcagcaca
      181 aggaagagat ggcggccgag gctggggaag ccgtggcgtc ccccatggac gacgggtttg
      241 tgagcctgga ctcgccctcc tatgtcctgt acaggcattt ccggagagtt cttttgaagt
      301 cacttcagaa ggatctacat gaggaaatga actacatcac tgcaataatt gaggagcagc
      361 ccaaaaacta tcaagtttgg catcataggc gagtattagt ggaatggcta agagatccat
      421 ctcaggagct tgaatttatt gctgatattc ttaatcagga tgcaaagaat tatcatgcct
      481 ggcagcatcg acaatgggtt attcaggaat ttaaactttg ggataatgag ctgcagtatg
      541 tggaccaact tctgaaagag gatgtgagaa ataactctgt ctggaaccaa agatacttcg
      601 ttatttctaa caccactggc tacaatgatc gtgctgtatt ggagagagaa gtccaataca
      661 ctctggaaat gattaaacta gtaccacata atgaaagtgc atggaactat ttgaaaggga
      721 ttttgcagga tcgtggtctt tccaaatatc ctaatctgtt aaatcaatta cttgatttac
      781 aaccaagtca tagttccccc tacctaattg cctttcttgt ggatatctat gaagacatgc
      841 tagaaaatca gtgtgacaat aaggaagaca ttcttaataa agcattagag ttatgtgaaa
      901 tcctagctaa agaaaaggac actataagaa aggaatattg gagatacatt ggaagatccc
      961 ttcaaagcaa acacagcaca gaaaatgact caccaacaaa tgtacagcaa ccaccggtcg
     1021 c
//
