LOCUS       sequence-175226-        6723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human androgen receptor N-terminal fragment 
            1-660 amino acids .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Simental JA, Sar M, Lane MV, French FS, Wilson EM
  TITLE     Transcriptional activation and nuclear targeting signals of the 
            human androgen receptor.
  JOURNAL   J Biol Chem. 1991 Jan 5;266(1):510-8.
  PUBMED    1985913
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1991-01-5"; volume: "266(1)"; pages: "510-8"
COMMENT     Sequence Label: pCMV-hAR1-660
FEATURES             Location/Qualifiers
     source          1..6723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    556..1178
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(755..774)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(1202..1222)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1207..1536
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1387..1522
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1574..1593)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1575..1593)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1607..1623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1607..1623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1620..1642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1631..1647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1655..1661,1662..1679,1680..1685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1700..1721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1838..1855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2009..2597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2089..2108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2768..3559,3560..3628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3391..3410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3629..3733)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3760..4215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3847..3866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4057..4078
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4341..4363
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4355..4372
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4356..4372
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4533..4912
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4913..5116
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5067..5087
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctgtagcccc ctacggctac actcggcccc ctcaggggct ggcgggccag gaaagcgact
       61 tcaccgcacc tgatgtgtgg taccctggcg gcatggtgag cagagtgccc tatcccagtc
      121 ccacttgtgt caaaagcgaa atgggcccct ggatggatag ctactccgga ccttacgggg
      181 acatgcgttt ggagactgcc agggaccatg ttttgcccat tgactattac tttccacccc
      241 agaagacctg cctgatctgt ggagatgaag cttctgggtg tcactatgga gctctcacat
      301 gtggaagctg caaggtcttc ttcaaaagag ccgctgaagg gaaacagaag tacctgtgcg
      361 ccagcagaaa tgattgcact attgataaat tccgaaggaa aaattgtcca tcttgtcgtc
      421 ttcggaaatg ttatgaagca gggatgactc tgggagcccg gaagctgaag aaacttggta
      481 atctgaaact acaggaggaa ggagaggctt ccagcaccac cagccccact gaggagacaa
      541 cccagaagct gacaggggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
      601 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
      661 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
      721 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
      781 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
      841 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
      901 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
      961 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     1021 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     1081 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     1141 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg
     1201 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     1261 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     1321 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     1381 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     1441 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     1501 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgagga actgaaaaac
     1561 cagaaagtta attccctata gtgagtcgta ttaaattcgt aatcatgtca tagctgtttc
     1621 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1681 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1741 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1801 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1861 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1921 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2521 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2581 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2641 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2701 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2761 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3241 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3301 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3361 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3421 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3481 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3541 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3721 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     3781 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3841 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3901 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3961 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4021 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4081 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4141 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4201 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     4261 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     4321 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
     4381 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     4441 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
     4501 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
     4561 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     4621 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     4681 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     4741 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
     4801 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
     4861 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     4921 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     4981 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     5041 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
     5101 ctatataagc agagctcgtt tagtgaaccg tcagaattca gatctgtcca ccgtgtgtct
     5161 tcttctgcac gagactttga ggctgtcaga gcgctttttg cgtggttgct cccgcaagtt
     5221 tccttctctg gagcttcccg caggtgggca gctagctgca gcgactaccg catcatcaca
     5281 gcctgttgaa ctcttctgag caagagaagg ggaggcgggg taagggaagt aggtggaaga
     5341 ttcagccaag ctcaaggatg gaagtgcagt tagggctggg aagggtctac cctcggccgc
     5401 cgtccaagac ctaccgagga gctttccaga atctgttcca gagcgtgcgc gaagtgatcc
     5461 agaacccggg ccccaggcac ccagaggccg cgagcgcagc acctcccggc gccagtttgc
     5521 tgctgctgca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     5581 agcagcagca gcagcagcag caagagacta gccccaggca gcagcagcag cagcagggtg
     5641 aggatggttc tccccaagcc catcgtagag gccccacagg ctacctggtc ctggatgagg
     5701 aacagcaacc ttcacagccg cagtcggccc tggagtgcca ccccgagaga ggttgcgtcc
     5761 cagagcctgg agccgccgtg gccgccagca aggggctgcc gcagcagctg ccagcacctc
     5821 cggacgagga tgactcagct gccccatcca cgttgtccct gctgggcccc actttccccg
     5881 gcttaagcag ctgctccgct gaccttaaag acatcctgag cgaggccagc accatgcaac
     5941 tccttcagca acagcagcag gaagcagtat ccgaaggcag cagcagcggg agagcgaggg
     6001 aggcctcggg ggctcccact tcctccaagg acaattactt agggggcact tcgaccattt
     6061 ctgacaacgc caaggagttg tgtaaggcag tgtcggtgtc catgggcctg ggtgtggagg
     6121 cgttggagca tctgagtcca ggggaacagc ttcgggggga ttgcatgtac gccccacttt
     6181 tgggagttcc acccgctgtg cgtcccactc cttgtgcccc attggccgaa tgcaaaggtt
     6241 ctctgctaga cgacagcgca ggcaagagca ctgaagatac tgctgagtat tcccctttca
     6301 agggaggtta caccaaaggg ctagaaggcg agagcctagg ctgctctggc agcgctgcag
     6361 cagggagctc cgggacactt gaactgccgt ctaccctgtc tctctacaag tccggagcac
     6421 tggacgaggc agctgcgtac cagagtcgcg actactacaa ctttccactg gctctggccg
     6481 gaccgccgcc ccctccgccg cctccccatc cccacgctcg catcaagctg gagaacccgc
     6541 tggactacgg cagcgcctgg gcggctgcgg cggcgcagtg ccgctatggg gacctggcga
     6601 gcctgcatgg cgcgggtgca gcgggacccg gttctgggtc accctcagcc gccgcttcct
     6661 catcctggca cactctcttc acagccgaag aaggccagtt gtatggaccg tgtggtggtg
     6721 gtg
//
