LOCUS       sequence-175227-        6786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human androgen receptor N-terminal fragment 
            1-660 amino acids .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Simental JA, Sar M, Lane MV, French FS, Wilson EM
  TITLE     Transcriptional activation and nuclear targeting signals of the 
            human androgen receptor.
  JOURNAL   J Biol Chem. 1991 Jan 5;266(1):510-8.
  PUBMED    1985913
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1991-01-5"; volume: "266(1)"; pages: "510-8"
COMMENT     Sequence Label: pCMV-hAR1-660
FEATURES             Location/Qualifiers
     source          1..6786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2810..3432
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3009..3028)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3456..3476)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3461..3790
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3641..3776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3828..3847)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3829..3847)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3861..3877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3861..3877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3874..3896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3885..3901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3909..3915,3916..3933,3934..3939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3954..3975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4092..4109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4263..4851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4343..4362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5022..5813,5814..5882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5645..5664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5883..5987)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6014..6469
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6101..6120)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6311..6332
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6595..6617
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6609..6626
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6610..6626
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcaga tctgtccacc gtgtgtcttc ttctgcacga gactttgagg ctgtcagagc
      661 gctttttgcg tggttgctcc cgcaagtttc cttctctgga gcttcccgca ggtgggcagc
      721 tagctgcagc gactaccgca tcatcacagc ctgttgaact cttctgagca agagaagggg
      781 aggcggggta agggaagtag gtggaagatt cagccaagct caaggatgga agtgcagtta
      841 gggctgggaa gggtctaccc tcggccgccg tccaagacct accgaggagc tttccagaat
      901 ctgttccaga gcgtgcgcga agtgatccag aacccgggcc ccaggcaccc agaggccgcg
      961 agcgcagcac ctcccggcgc cagtttgctg ctgctgcagc agcagcagca gcagcagcag
     1021 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcaagagac tagccccagg
     1081 cagcagcagc agcagcaggg tgaggatggt tctccccaag cccatcgtag aggccccaca
     1141 ggctacctgg tcctggatga ggaacagcaa ccttcacagc cgcagtcggc cctggagtgc
     1201 caccccgaga gaggttgcgt cccagagcct ggagccgccg tggccgccag caaggggctg
     1261 ccgcagcagc tgccagcacc tccggacgag gatgactcag ctgccccatc cacgttgtcc
     1321 ctgctgggcc ccactttccc cggcttaagc agctgctccg ctgaccttaa agacatcctg
     1381 agcgaggcca gcaccatgca actccttcag caacagcagc aggaagcagt atccgaaggc
     1441 agcagcagcg ggagagcgag ggaggcctcg ggggctccca cttcctccaa ggacaattac
     1501 ttagggggca cttcgaccat ttctgacaac gccaaggagt tgtgtaaggc agtgtcggtg
     1561 tccatgggcc tgggtgtgga ggcgttggag catctgagtc caggggaaca gcttcggggg
     1621 gattgcatgt acgccccact tttgggagtt ccacccgctg tgcgtcccac tccttgtgcc
     1681 ccattggccg aatgcaaagg ttctctgcta gacgacagcg caggcaagag cactgaagat
     1741 actgctgagt attccccttt caagggaggt tacaccaaag ggctagaagg cgagagccta
     1801 ggctgctctg gcagcgctgc agcagggagc tccgggacac ttgaactgcc gtctaccctg
     1861 tctctctaca agtccggagc actggacgag gcagctgcgt accagagtcg cgactactac
     1921 aactttccac tggctctggc cggaccgccg ccccctccgc cgcctcccca tccccacgct
     1981 cgcatcaagc tggagaaccc gctggactac ggcagcgcct gggcggctgc ggcggcgcag
     2041 tgccgctatg gggacctggc gagcctgcat ggcgcgggtg cagcgggacc cggttctggg
     2101 tcaccctcag ccgccgcttc ctcatcctgg cacactctct tcacagccga agaaggccag
     2161 ttgtatggac cgtgtggtgg tggtgggggt ggtggcggcg gcggcggcgg cggcggcggc
     2221 ggcggcggcg gcggcggcgg cggcgaggcg ggagctgtag ccccctacgg ctacactcgg
     2281 ccccctcagg ggctggcggg ccaggaaagc gacttcaccg cacctgatgt gtggtaccct
     2341 ggcggcatgg tgagcagagt gccctatccc agtcccactt gtgtcaaaag cgaaatgggc
     2401 ccctggatgg atagctactc cggaccttac ggggacatgc gtttggagac tgccagggac
     2461 catgttttgc ccattgacta ttactttcca ccccagaaga cctgcctgat ctgtggagat
     2521 gaagcttctg ggtgtcacta tggagctctc acatgtggaa gctgcaaggt cttcttcaaa
     2581 agagccgctg aagggaaaca gaagtacctg tgcgccagca gaaatgattg cactattgat
     2641 aaattccgaa ggaaaaattg tccatcttgt cgtcttcgga aatgttatga agcagggatg
     2701 actctgggag cccggaagct gaagaaactt ggtaatctga aactacagga ggaaggagag
     2761 gcttccagca ccaccagccc cactgaggag acaacccaga agctgacagg ggtggcatcc
     2821 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag
     2881 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
     2941 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
     3001 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat
     3061 ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca
     3121 ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat
     3181 attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa
     3241 attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata
     3301 actataccag caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt
     3361 gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat
     3421 gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt tagggtgtgg
     3481 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3541 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3601 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3661 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3721 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3781 cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa gttaattccc tatagtgagt
     3841 cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     3901 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     3961 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4021 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4081 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4141 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4201 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4261 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4321 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4381 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4441 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4501 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4561 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4621 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4681 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4741 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4801 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4861 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4921 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4981 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5041 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5101 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5161 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5221 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5281 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5341 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5401 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5461 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5521 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5581 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5641 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5701 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5761 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5821 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5881 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5941 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6001 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6061 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6121 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6181 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6241 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6301 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6361 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6421 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat
     6481 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     6541 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     6601 cacgacgttg taaaacgacg gccagtgcca agctgatcta tacattgaat caatattggc
     6661 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     6721 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
     6781 catgtt
//
