LOCUS       sequence-175229-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express FNTB with a HA tag at C terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pcDNA5/FRT/TO-FNTB-HA
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      939..948
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc
      181 gagctcggat ctaccgccat ggcttctccg agttctttca cctactattg ccctccatct
      241 tcctcccccg tctggtcaga gccgctgtac agtctgaggc ccgagcacgc gcgagagcgg
      301 ttgcaggacg actcggtgga aacagtcacg tccatagaac aggcaaaagt agaagaaaag
      361 atccaagagg tcttcagttc ttacaagttc aaccaccttg taccaaggct tgttttgcag
      421 agggagaagc acttccatta tctgaaaaga ggccttcgac aactgacaga tgcctatgag
      481 tgtctggatg ccagccgccc atggctctgc tattggatcc tgcacagctt ggaactgcta
      541 gatgaaccca tcccccagat agtggctaca gatgtgtgtc agttcctgga gctgtgtcag
      601 agcccagaag gtggctttgg aggaggaccc ggtcagtatc cacaccttgc acccacatat
      661 gcagcagtca atgcattgtg catcattggc accgaggagg cctatgacat cattaacaga
      721 gagaagcttc ttcagtattt gtactccctg aagcaacctg acggctcctt tctcatgcat
      781 gtcggaggtg aggtggatgt gagaagcgca tactgtgctg cctccgtagc ctcgctgacc
      841 aacatcatca ctccagacct ctttgagggc actgctgaat ggatagcaag gtgtcagaac
      901 tgggaaggtg gcattggcgg ggtaccaggg atggaagccc atggtggcta taccttctgt
      961 ggcctggccg cgctggtaat cctcaagagg gaacgttcct tgaacttgaa gagcttatta
     1021 caatgggtga caagccggca gatgcgattt gaaggaggat ttcagggccg ctgcaacaag
     1081 ctggtggatg gctgctactc cttctgg
//
