LOCUS       sequence-175230-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express FNTB with a HA tag at C terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pcDNA5/FRT/TO-FNTB-HA
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      486..495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1057..1083
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cttcgacaac tgacagatgc ctatgagtgt ctggatgcca gccgcccatg gctctgctat
       61 tggatcctgc acagcttgga actgctagat gaacccatcc cccagatagt ggctacagat
      121 gtgtgtcagt tcctggagct gtgtcagagc ccagaaggtg gctttggagg aggacccggt
      181 cagtatccac accttgcacc cacatatgca gcagtcaatg cattgtgcat cattggcacc
      241 gaggaggcct atgacatcat taacagagag aagcttcttc agtatttgta ctccctgaag
      301 caacctgacg gctcctttct catgcatgtc ggaggtgagg tggatgtgag aagcgcatac
      361 tgtgctgcct ccgtagcctc gctgaccaac atcatcactc cagacctctt tgagggcact
      421 gctgaatgga tagcaaggtg tcagaactgg gaaggtggca ttggcggggt accagggatg
      481 gaagcccatg gtggctatac cttctgtggc ctggccgcgc tggtaatcct caagagggaa
      541 cgttccttga acttgaagag cttattacaa tgggtgacaa gccggcagat gcgatttgaa
      601 ggaggatttc agggccgctg caacaagctg gtggatggct gctactcctt ctggcaggcg
      661 gggctcctgc ccctgctcca ccgcgcactg cacgcccaag gtgaccctgc ccttagcatg
      721 agccactgga tgttccatca gcaggccctg caggagtaca tcctgatgtg ctgccagtgc
      781 cctgcggggg ggcttctgga taaacctggc aagtcgcgtg atttctacca cacctgctac
      841 tgcctgagcg gcctgtccat agcccagcac ttcggcagcg gagccatgtt gcatgatgtg
      901 gtcctgggtg tgcccgaaaa cgctctgcag cccactcacc cagtgtacaa cattggacca
      961 gacaaggtga tccaggccac tacatacttt ctacagaagc cagtcccagg ttttgaggag
     1021 cttaaggatg agacatcggc agagcctgca accgactacc catacgatgt tccagattac
     1081 gcttaactcg agtctagag
//
