LOCUS       sequence-175232-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, 
            respectively..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pEGFP-C1-GFP-SF3B6-FLAG
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             445..468
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    601..722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(638..657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     692..711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(866..887)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattct
       61 gcagtcgaca tggcgatgca agcggccaag agggcgaaca ttcgacttcc acctgaagta
      121 aatcggatat tgtatataag aaatttgcca tacaaaatca cagctgaaga aatgtatgat
      181 atatttggga aatatggacc tattcgtcaa atcagagtgg ggaacacacc tgaaactaga
      241 ggaacagctt atgtggtcta tgaggacatc tttgatgcca agaatgcatg tgatcaccta
      301 tcgggattca atgtttgtaa cagatacctt gtggttttgt actataatgc caacagggca
      361 tttcagaaga tggacacaaa gaagaaggag gaacagttga agcttctcaa ggagaaatat
      421 ggcatcaaca cagatccacc aaaagactac aaggacgacg atgacaagta aggatccacc
      481 ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
      541 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
      601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
      661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
      721 taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      781 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      841 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      901 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
      961 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1021 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaag
//
