LOCUS       sequence-175235-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- 
            and C-terminus, respectively..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             376..399
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    532..653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(569..588)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     623..642
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(797..818)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattct
       61 gcagtcgaca tggcgatgca agcggccatc acagctgaag aaatgtatga tatatttggg
      121 aaatatggac ctattcgtca aatcagagtg gggaacacac ctgaaactag aggaacagct
      181 tatgtggtct atgaggacat ctttgatgcc aagaatgcat gtgatcacct atcgggattc
      241 aatgtttgta acagatacct tgtggttttg tactataatg ccaacagggc atttcagaag
      301 atggacacaa agaagaagga ggaacagttg aagcttctca aggagaaata tggcatcaac
      361 acagatccac caaaagacta caaggacgac gatgacaagt aaggatccac cggatctaga
      421 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
      481 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
      541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
      601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
      661 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
      721 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
      781 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
      841 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
      901 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
      961 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaa
//
