LOCUS       sequence-175238-        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express POLR2G (RPB7) tagged with VENUS at C terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pVENUS-N1-POLR2G-GFP
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      606..615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(612..614,615..617,618..1076)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(657..678)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(918..937)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttgc caccatgttc taccatatct ccctagagca cgaaatcctg ctgcacccgc
      121 gctacttcgg ccccaacttg ctcaacacgg tgaagcagaa gctcttcacc gaggtggagg
      181 ggacctgcac agggaagtat ggctttgtaa ttgctgtcac caccattgac aatattggtg
      241 ctggtgtgat ccagccaggc cgaggctttg tcctttatcc agttaagtac aaggccattg
      301 ttttccggcc atttaaaggg gaggtcgtgg atgctgttgt cactcaggtc aacaaggttg
      361 gactcttcac agaaattggg cccatgtctt gcttcatctc tcgacattcc atcccttcag
      421 agatggagtt tgatcctaac tccaacccac catgttacaa gacaatggat gaggatattg
      481 tgattcagca ggacgatgag atccgcttaa agattgtggg gacccgtgtg gacaagaatg
      541 acatttttgc tattggctcc ctgatggacg attacttggg gcttgtaagc cgggatccac
      601 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      661 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      721 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
      781 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
      841 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      901 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      961 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1021 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     1081 agcagaagaa cggcatcaag gccaacttca agatcc
//
