LOCUS       sequence-175239-        4638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of FKBP5 also referred to as FKBP51 with 
            N-terminal VP16 activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Su S, Blackwelder AJ, Grossman G, Minges JT, Yuan L, Young SL, 
            Wilson EM
  TITLE     Primate-specific melanoma antigen-A11 regulates isoform-specific 
            human progesterone receptor-B transactivation.
  JOURNAL   J Biol Chem. 2012 Oct 5;287(41):34809-24. doi: 
            10.1074/jbc.M112.372797. Epub 2012 Aug 13.
  PUBMED    22891251
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.372797"; journalName: "J Biol Chem"; date: 
            "2012-10-5- 5"; volume: "287"; issue: "41"; pages: "34809-24"
COMMENT     Sequence Label: VP-FKBP51
FEATURES             Location/Qualifiers
     source          1..4638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(29..49)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     regulatory      357..366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             369..389
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             390..623
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     polyA_signal    2148..2282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2185..2204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2239..2258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2292..2311)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2293..2311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2398..2415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2569..3157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2649..2668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3328..4119,4120..4188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3951..3970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4189..4293)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4361..4379
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4417..4439)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4539..4558
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atttaggtga cactatagaa ctcgatgtgg aatgtgtgtc agttagggtg tggaaagtcc
       61 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaggaaa
      121 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      181 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
      241 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      301 tgagctattc cagaagtagt gaagaggctt ttttggagga gatctaagct agcgccgcca
      361 ccatgggccc taaaaagaag cgtaaagtcg cccccccgac cgatgtcagc ctgggggacg
      421 agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg
      481 atctggacat gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg
      541 ccccctacgg cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc
      601 ttggaattga cgagtacggt ggggaattca caatgactac tgatgaaggt gccaagaaca
      661 atgaagaaag ccccacagcc actgttgctg agcagggaga ggatattacc tccaaaaaag
      721 acaggggagt attaaagatt gtcaaaagag tggggaatgg tgaggaaacg ccgatgattg
      781 gagacaaagt ttatgtccat tacaaaggaa aattgtcaaa tggaaagaag tttgattcca
      841 gtcatgatag aaatgaacca tttgtcttta gtcttggcaa aggccaagtc atcaaggcat
      901 gggacattgg ggtggctacc atgaagaaag gagagatatg ccatttactg tgcaaaccag
      961 aatatgcata tggctcggct ggcagtctcc ctaaaattcc ctcgaatgca actctctttt
     1021 ttgagattga gctccttgat ttcaaaggag aggatttatt tgaagatgga ggcattatcc
     1081 ggagaaccaa acggaaagga gagggatatt caaatccaaa cgaaggagca acagtagaaa
     1141 tccacctgga aggccgctgt ggtggaagga tgtttgactg cagagatgtg gcattcactg
     1201 tgggcgaagg agaagaccac gacattccaa ttggaattga caaagctctg gagaaaatgc
     1261 agcgggaaga acaatgtatt ttatatcttg gaccaagata tggttttgga gaggcaggga
     1321 agcctaaatt tggcattgaa cctaatgctg agcttatata tgaagttaca cttaagagct
     1381 tcgaaaaggc caaagaatcc tgggagatgg ataccaaaga aaaattggag caggctgcca
     1441 ttgtcaaaga gaagggaacc gtatacttca agggaggcaa atacatgcag gcggtgattc
     1501 agtatgggaa gatagtgtcc tggttagaga tggaatatgg tttatcagaa aaggaatcga
     1561 aagcttctga atcatttctc cttgctgcct ttctgaacct ggccatgtgc tacctgaagc
     1621 ttagagaata caccaaagct gttgaatgct gtgacaaggc ccttggactg gacagtgcca
     1681 atgagaaagg cttgtatagg aggggtgaag cccagctgct catgaacgag tttgagtcag
     1741 ccaagggtga ctttgagaaa gtgctggaag taaaccccca gaataaggct gcaagactgc
     1801 agatctccat gtgccagaaa aaggccaagg agcacaacga gcgggaccgc aggatatacg
     1861 ccaacatgtt caagaagttt gcagagcagg atgccaagga agaggccaat aaagcaatgg
     1921 gcaagaagac ttcagaaggg gtcactaatg aaaaaggaac agacagtcaa gcaatggaag
     1981 aagagaaacc tgagggccac gtataggtcg acgcgtctgc agaagcttct agataagtaa
     2041 tgatcataat cagccatatc acatctgtag aggttttact tgctttaaaa aacctcccac
     2101 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2161 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2221 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     2281 tctgccggtc tccctatagt gagtcgtatt aatttcgata agccaggtta acctgcatta
     2341 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2401 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2461 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2521 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2581 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2641 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2701 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2761 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2821 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2881 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2941 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3001 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3061 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3121 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3181 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3241 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3301 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3361 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3421 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3481 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3541 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3601 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3661 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3721 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3781 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3841 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3901 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3961 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4021 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4081 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4141 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4201 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4261 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4321 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4381 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4441 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4501 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     4561 gagagtgcac catatggaca tattgtcgtt agaacgcggc tacaattaat acataacctt
     4621 atgtatcata cacatacg
//
