LOCUS       sequence-175240-        9488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Yang J, Mani SA, Donaher JL, Ramaswamy S, Itzykson RA, Come C, 
            Savagner P, Gitelman I, Richardson A, Weinberg RA
  TITLE     Twist, a master regulator of morphogenesis, plays an essential role 
            in tumor metastasis.
  JOURNAL   Cell 2004 Jun 25;117(7):927-39.
  PUBMED    15210113
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2004-06-25"; volume: "117(7)"; pages: "927-39"
COMMENT     Sequence Label: Twist-siRNA3
FEATURES             Location/Qualifiers
     source          1..9488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(605..1396,1397..1465))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1228..1247
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1466..1570)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1638..1656
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1694..1716)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1816..1835
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2049..2068
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2358..2376
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2358..2375
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             2401..2634
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    2727..3315
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2833..2844
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3242..3262
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3386..3985)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3469..3489)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3966..3985
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3995..4324)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4009..4144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4356..4596
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     4356..4376
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4527..4546
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     CDS             complement(4923..4967)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5152..5385
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5878..6003
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6050..6683
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6851..6985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6875..6894)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6929..6948
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7410..7430)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7560..7625
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(8759..9088)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8773..8908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9163..9180)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9334..9488,1..434))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9414..9433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
       61 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      121 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      181 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      241 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      301 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      361 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      421 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      481 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      541 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      601 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      661 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
      721 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
      781 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
      841 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
      901 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
      961 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1021 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1081 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1141 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1201 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1261 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1321 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1381 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1441 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1501 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1561 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1621 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1681 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     1741 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     1801 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat tcgacgctct
     1861 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     1921 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     1981 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     2041 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     2101 ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct gattggttcg
     2161 ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg tccaaccaaa
     2221 ccgactctga cggcagttta cgagagagat gatagggtct gcttcagtaa gccagatgct
     2281 acacaattag gcttgtacat attgtcgtta gaacgcggct acaattaata cataacctta
     2341 tgtatcatac acatacgatt taggtgacac tatagaatac acggaattaa ttctagatgc
     2401 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     2461 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     2521 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     2581 cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc
     2641 ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag
     2701 gtaccgagct cgaattaatt cccgatgcgg ggaggcggcc caaagggaga tccgactcgt
     2761 ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg
     2821 aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca
     2881 ggtggcaaca caggcgagca gccatggaaa ggacgtcagc ttccccgaca acaccacgga
     2941 attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag
     3001 ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt
     3061 ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg
     3121 cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat acaaggagga
     3181 gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc
     3241 acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta
     3301 atccagaggt tgattatcgg aattaattcg atgcatgggg tcgtgcgctc ctttcggtcg
     3361 ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg caccaggtgc
     3421 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg
     3481 ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct
     3541 ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc
     3601 cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca
     3661 tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg
     3721 cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt
     3781 cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca
     3841 gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt
     3901 cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc
     3961 gcaccgtggg cttgtactcg gtcatggtaa gctttttgca aaagcctagg cctccaaaaa
     4021 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     4081 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     4141 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
     4201 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
     4261 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
     4321 acacattcca cagggtcgac aaggtcgggc aggaagaggg cctatttccc atgattcctt
     4381 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
     4441 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     4501 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     4561 tcgatttctt ggctttatat atcttgtgga aaggacgatt cgaaaagctg agcaagattc
     4621 agaccttcaa gagaggtctg aatcttgctc agcttttgga tccgataccg tcgagatccg
     4681 ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg
     4741 ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     4801 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
     4861 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
     4921 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
     4981 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
     5041 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
     5101 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
     5161 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
     5221 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
     5281 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
     5341 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
     5401 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
     5461 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca
     5521 ggtctgaaga tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc
     5581 tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag
     5641 ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg
     5701 tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc
     5761 gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc
     5821 ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac
     5881 gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac
     5941 cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga
     6001 gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt
     6061 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     6121 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     6181 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca
     6241 aaaagcagct gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc
     6301 acgcctccct ggaaagtccc cagcggaaag tcccttgtag caagctcgat atcagcagtt
     6361 cttgaagtac tccggatgca gctctcgggc cacgtgatga aatgctaggc ggctgtcaaa
     6421 cctccactct aacacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa
     6481 caagctggtg ttctctcctt tattggcctc ttctacctta tctggctcaa ctggtactag
     6541 cttgtagcac catccaaagg tcagtggata tctgacccct ggccctggtg tgtagttctg
     6601 ctaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttct
     6661 ttgggagtga attagccctt ccaactacta agtttgtagt acatatttaa caaatacaat
     6721 ttctttaaaa tgaaaataat tcagaggaat cacaggttta gagtaaatga aaccacaggt
     6781 aattggcagt ggtaataggg tatggggtgg gaagtttggg atgattttgg ttagcttgag
     6841 ttatccagtt gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6901 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6961 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     7021 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     7081 tgattatgat ctctagtcaa ggcactatac atcaaatatt ccttattaac ccctttacaa
     7141 attaaaaagc taaaggtaca caatttttga gcatagttat taatagcaga cactctatgc
     7201 ctgtgtggag taagaaaaaa cagtatgtta tgattataac tgttatgcct acttataaag
     7261 gttacagaat atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg
     7321 taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc
     7381 aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa
     7441 tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg
     7501 ttttcctcat taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc
     7561 taaaatacac aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata
     7621 tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac accacagaag
     7681 taaggttcct tcacaaagat ccggggccca ctcataaatc cagttgccgc cacggtagcc
     7741 aatcaccgta tcgtataaat catcgtcggt acgttcggca tcgctcatca caatacgtgc
     7801 ctggacgtcg aggatttcgc gtgggtcaat gccgcgccag atccacatca gacggttaat
     7861 catgcgatac cagtgaggga tggttttacc atcaagggcc gactgcacag gcggttgtgc
     7921 gccgtgatta aagcggcgga ctagcgtcga ggtttcagga tgtttaaagc ggggtttgaa
     7981 cagggtttcg ctcaggtttg cctgtgtcat ggatgcagcc tccagaatac ttactggaaa
     8041 ctattgtaac ccgcctgaag ttaaaaagaa caacgcccgg cagtgccagg cgttgaaaag
     8101 attagcgacc ggagattggc gggacgaata cgacgcccat atcccacggc tgttcaatcc
     8161 aggtatcttg cgggatatca acaacatagt catcaaccag cggacgacca gccggttttg
     8221 cgaagatggt gacaaagtgc gcttttggat acatttcacg aatcgcaacc gcagtaccac
     8281 cggtatccac caggtcatca ataacgatga agccttcgcc atcgccttct gcgcgtttca
     8341 gcactttaag ctcgcgctgg ttgtcgtgat cgtagctgga aatacaaacg gtatcgacat
     8401 gacgaatacc cagttcacgc gccagtaacg cacccggtac agaccgccac ggcttacggc
     8461 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     8521 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     8581 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     8641 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     8701 cgcgggataa agtggtattc tcaaacatat ctcgcaagcc tgtcttgtgt ccaagctttt
     8761 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     8821 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     8881 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
     8941 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     9001 acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     9061 gggactttcc acaccctaac tgacacacat tccacagctg cattaatgaa tcggccaacg
     9121 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9181 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9241 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9301 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9361 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9421 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9481 cggatacc
//
