LOCUS       sequence-175242-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express GRB2 with a GFP tag at C terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pcDNA5/FRT/TO-GRB2-GFP
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc
      181 gagctcggat cccaccatgg aagccatcgc caaatatgac ttcaaagcta ctgcagacga
      241 cgagctgagc ttcaaaaggg gggacatcct caaggttttg aacgaagaat gtgatcagaa
      301 ctggtacaag gcagagctta atggaaaaga cggcttcatt cccaagaact acatagaaat
      361 gaaaccacat ccgtggtttt ttggcaaaat ccccagagcc aaggcagaag aaatgcttag
      421 caaacagcgg cacgatgggg cctttcttat ccgagagagt gagagcgctc ctggggactt
      481 ctccctctct gtcaagtttg gaaacgatgt gcagcacttc aaggtgctcc gagatggagc
      541 cgggaagtac ttcctctggg tggtgaagtt caattctttg aatgagctgg tggattatca
      601 cagatctaca tctgtctcca gaaaccagca gatattcctg cgggacatag aacaggtgcc
      661 acagcagccg acatacgtcc aggccctctt tgactttgat ccccaggagg atggagagct
      721 gggcttccgc cggggagatt ttatccatgt catggataac tcagacccca actggtggaa
      781 aggagcttgc cacgggcaga ccggcatgtt tccccgcaat tatgtcaccc ccgtgaaccg
      841 gaacgtcgat atcatggtga gcaagggcga ggagctgttc accggggtgg tg
//
