LOCUS       sequence-175246-        6356 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Complements mutations in Aspergillus nidulans gene choA (AN2154) 
            (mutants require choline).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Dohn JW Jr, Grubbs AW, Oakley CE, Oakley BR
  TITLE     New multi-marker strains and complementing genes for Aspergillus 
            nidulans molecular biology.
  JOURNAL   Fungal Genet Biol. 2018 Feb;111:1-6. doi: 10.1016/j.fgb.2018.01.003.
            Epub 2018 Jan 5.
  PUBMED    29309843
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-02"; volume: "111:1-6. doi"; pages: " 
            10.1016/j.fgb.2018.01.003. Epub 2018 Jan 5"
COMMENT     Sequence Label: pLO102
FEATURES             Location/Qualifiers
     source          1..6356
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctgtctgaga ggaggcactg tgtggagtcg gcgctagttt ttcctctcac tttttcctct
       61 ttttcttctc ctcctccttt cttctcttcc tcgcttctct tccacacaac atccgcttta
      121 tctctggcga attgaccttt taatgacgaa ggctcaatcc agctacatta gcaccttttc
      181 ttctcttcca ggatactaac ctttactgta agttgtccca tcatcgcttc caccgcacaa
      241 tgcattctat gaggaaaacg atccaaaaat cttagttcgg cgacaaacaa atgggccgtg
      301 aacaacatgc actaccatct gaaaatgctt tctcatctca cttttatctt agcctgttct
      361 tgggaactgt tggctaactt aatatcagac aggtagctga gctctcagtg gctatttcac
      421 atcaaacctg tagactcttc gaaaggtatg ccttttattc cttttctttt cggcctcgtc
      481 tttcccccac ccgtttccat gatgagaaac attttttaca gttctgcttc tgcaaactca
      541 ttgaagaaaa ccaaatacct taccaaatct actgatatgg aaactccctt cttgttcatc
      601 acgacacaat cacatcaccc atgccgaccc aagctaacag cttcaagata cagatatcgt
      661 agtctcagct cgcatcacgt cagccactag cacactagca taggtatgaa ctaccacgca
      721 gacaatcctc cctcacacca ctccttgatg atgacaacaa gtcaatccat tcaatgattc
      781 tcttcggaga aaccgcccct cgagcctgca tcggcttcca cacatcgcac ttgaagtgca
      841 acgatccgct tgcccacctc ccttgtactg gacatttcgt cgtccatgtc tttctcggaa
      901 gacacggtcg atgaaatgga ggatggttgt ggagcgttgg gtggcgggct ccctcgttgt
      961 gtcttccgcg tttgttcctg gctgcgtgtt gctgtgcttg acttgtgtct ttgtcggttt
     1021 ctcggcttgt cgtccgtcct ggatggctcg cgggggggtc gaaattggca ggggatggga
     1081 tgaatttacc ggcttggctg atcctttcgc tatactaccg gtcgccttct atctggcggc
     1141 aggtggtttc tggcttctac cactcgacag gattgcgagc ttatgctaac tgtctttgta
     1201 gattcgtgtc agctttcata tagtacccct aggtagataa cttggaaatg aaacatagcc
     1261 attctacgaa ctatatcttt atctagaaaa cgataattca ttcataatat ataccacgaa
     1321 gctcaatgta gtactttaca taccccccag caagctccgg cagaagctca caacatcagg
     1381 tctcatcagg aaacccctgc aggcaaccat cttctccaca tcccatccgt ctccatctat
     1441 tcggatttta ttcgccatgt agcttcacca ttcgggttca aaagcaatcg tatgtgatca
     1501 aggcaggtga aaggaaagaa aacgaccgat gcgacaaaca gacacgcagc atctaagacc
     1561 tatctatgaa aagctttcgt ggctcatatt caaagaaaca gcagagaagc aaatcgtcgt
     1621 gatagcattt gttcaatagg gagggaggac acgcaaggtg taggcgtatc cgctttattc
     1681 ttttctttcg gtcggggtgg acgcaccgtt gcgcgacata ctgtaaggcg cctgtcatag
     1741 gagcagttat tagaagccac gctggtgatt tatacgtgga gaaaaactta ccaaaacctt
     1801 ctttgcattg catattcgcc aagcaaggtt gcgtacagtt ccatcggcgc ggacgacttc
     1861 cggggcgaat tcgatgccga acctgaacac cgtgttaaca tgcactcggt agacgatatt
     1921 atgataggaa aataggaagc cttacatctg atgcacggca aaaacaaccc gcttggcata
     1981 cttgccgtca cgttcgacca gggatccaaa ctgttcgtcg acggtctcag gcgcgacctc
     2041 tgggtctcgg tcaaagcagt tgcgaacgac tggcaacaga ctattttcaa cagaggtctg
     2101 catcatctca tggtggcctt cttcctcaaa ccggcggatg cggatttcaa acggtcttga
     2161 gatcgccatc acgttatgct tgccattgtg gtggtaccgg aactcgaaga cgccttgggt
     2221 ccagaatagc ttgtcgctgg agaagatgac ttcccccgag gccatctcgc ggttgtcccc
     2281 atgcggagct gggatgacca catcgctcga aacaatcccc ttttcgcagg tcaaattgtc
     2341 gtaggatcct tcattcaccg cgatccatcg tccttgggaa gaaacctttg tgatctcccg
     2401 ggaagtgtta tcggtcacgc gataaagtcc aatccaatct ttcttgctgt gattgagagg
     2461 cgcagtccat ttgaccttga tcggggctcc gtattcgaat accaagtttt tcagatcacc
     2521 gcgtctatca aggggcatgc gggcattagc accctctcta ccactacttt ggtcgcattc
     2581 ggcaagagct gatgaatcgg tgccttcaac ctccagagag taatctcgcg aatcatatcc
     2641 ggccacgtcc tcatcgatgc gggtgatggt aacacgggcg gggtacttct ggaagagggc
     2701 ggttgtatcc ttcacgaacg tgtcaacacc ggcagcaagc ttggggcggg cattttccaa
     2761 gaaatcctct ataaactcga aagaaccgtc gacgattttg tccatactcc catgaagttg
     2821 cttgagagga ggaggcaagg accgtttcag gttcttaaca agaccggcat cgcgacggat
     2881 actctggcca tagagctttt gcatgtgtgg acgctcaatg aactgaatga acgccaggct
     2941 gaggatgtgg ctcagaagcg cgaggaacgt gattgctcca ctactagtga taaggaccgc
     3001 accccagacc ccggcaagac caaggactcg ctctgggttg ttcaggaagc gatagatccc
     3061 gttgtacgtc aacttgggcg actcgtcgta gaagaagtct ccgtagaacc agccaaattc
     3121 tccaagggac tcatagatac taacggacgt ccagatttgt aggcagatca gccctgcccc
     3181 gagaacgtgc cggagtaaga caaggccata gccccagtcg gaggggagag tatacatttt
     3241 ccatacagca gcaatgaagc tcgcgtagca catgatcatg cttaggtgat acgtgccctt
     3301 ccactggttc cacgcctcat ggggtgtttc gccatacttg acgaaatgcc gggtccaagc
     3361 tttgcagttg gattggcgat tgaggaggta tccaatgcca acagagtacc agagcctcca
     3421 caccgctgca ttggccacga acaggaactg ataccagggc gtggagggcg taaggacagt
     3481 gacagcgaat actaggaatt ggaccagcat ggacgaagta tcggttatgc gatacagatc
     3541 caaattgtgg aagcctaaca gattgtggac cgatggagga ggccggcttg aaaacgttgg
     3601 tgcgtggggc acattttcat caacgggtgc tggcgcgatg ggagagtccg tggtgtgggt
     3661 agtcgtggaa gccgtatcct ggtcgattgt acgtttgcgg ggtggtgggg gattgtacgt
     3721 cttgtcaatg tgtgggtttt cgacgaagac caagaaagcg aactgagctg cgtgggcgat
     3781 gatagagatg aacagcacct tgtagctagc ggccattagc gagataccgt agtaccccgc
     3841 gtaaccaacc gagtacatcg ggtggggcgc catctcgaag acaccgtcga atgtcaattc
     3901 ctggtcgatc aggtagaaga agtcgcccca gtaccacgca tagtccttta ccacacgatg
     3961 cgcgtctaac ttgacccaga ggttgaagag caccagcaca atgccggaga accacctcag
     4021 cacggtcatt agcaggctct cattgacagg atggtgacta caggctatcg caaagagaca
     4081 gtacgatgta aagtcacaca tcaagatcaa atcgaccagg cgcctaaaaa cgagccaggt
     4141 gttgtactcg agaggtgcct tctcgaaaga atagtcgtgg gggatcttgg tttccagttc
     4201 ccgtttgatc aggttgtaca gatgagggtg cggattctgt cccgtcgcag gattgacgaa
     4261 gatcttggtt ttctcagccc agcgaacgag ggttttgtgg tgggattggt tgtgaagaag
     4321 ccatccaatc cccgcattgt agcccgcacg ccagaagaga tacaagaccg cagagacagg
     4381 aaccttcgca cctttgggca gcgcccagat aaggagaata tgagcaccca agatagccag
     4441 gacgatgata tctgataggt tcttcggctc tgaaggcgac aggagctggg aaaccatgtc
     4501 atgtgtttgg ggcacagtaa atactgagaa tccatcatgg ttagcactga gtataggtga
     4561 ggatgccagc caaacgccgc agaggcgaca aaattgaccg gcccaacaga tttatagatg
     4621 tacagagcaa ggacttacct gtgccatccg gggtgcgtcc ataggtcttc aattccttcc
     4681 cagtgtcctt cggctctacg tcaccagtgg cggtaagagc ctcgggactg agcgttgaag
     4741 ccgattgaga ggcaacgggt ctctcccgaa gcccctcgtt atcattgtga gtgcctgttg
     4801 aaaggccacg atccatgatt gtggtttggg gttgatgaag aaagagaggg ggaatcaaag
     4861 ccaaaaacac gaccaggatt caaccacgag gtcaccagca tgtgacagcc ggaaagggtt
     4921 ggggtactta agcggccaga tcttgattgg tttacgtcat gtgagacgcc agtcccgatt
     4981 tagttagccg cggccgtggt ggtatgtcga attcgacttc tttgaaagtt gaaaactgcg
     5041 atggtcaaga cagagggaaa ggggaaccag aaggggatta aatatggcgg gcgagaggaa
     5101 gaagaggaga ggacgaggag gaaaacttgt tgcagaaaac aggggagaag gaggggtgtt
     5161 gacgatttac atcgagaggg acaaccgaga caggcccgtg tttattttag caccataccg
     5221 taatgaccaa aatgccacaa tcccctacct agtcggtacg ggtatcaata agggactaca
     5281 ccgtaagtaa ctcatactac ctaacggtaa tcgcgcagtc agctatccat tcaagtgctc
     5341 tctagctgct gcgtacaggt aactattctc gccgactctc ggagtggaac ccccctccgc
     5401 tggggcagag aaacggggct ccgttgagtc ggaaaacgcc aatcagagca gccatgaggg
     5461 ctacagcgaa gaatgagacg gaaatagaga gaagggcaac tagtaatgtc atagcacgct
     5521 acgaataatg catcttattc tgcgtattaa acaacgcaag aacaaccggc aggaaaccaa
     5581 gccctgagat agctacctag cttagtccgc atctacccga aggttggaca tggcatacgt
     5641 agtaattctg ttctctacag cggtaagagg cgacaaaaaa aaaggagggc taaagcatag
     5701 acgcaaaaag gtttcgcgac aacggcagga ttcgaaccta cgcctcctaa gagacagcct
     5761 tagcagggct gcgtgttaac cactccacca cgttgtcatt acgtaggcaa aatgagtgct
     5821 taagcaattg taggggtagg ggcttcaggt cggacgattg gcatgccgca ctagaccgcc
     5881 taccgtttaa agagaactgt tactctgcat accccatagt agtaaaagaa tgtcgacgcc
     5941 gctccaaccg gatagttaag aaacgagact tccggggaat ttctccgatg ctatgcttgt
     6001 tcgtgcgaag gccgttgaac cgaaattacc atacacgata aactgcatac cgattgatgt
     6061 tgttaggtag gttctggttg gcaaaattgt gtcgccagct tttccagtct gccctcccgt
     6121 caaaatcaac ctgctcccac tgtacggaat aacctcctac catgacatgg cgatgcgttg
     6181 ggcaaacaaa aatatgcgac aacggcagga ttcgaaccta cgcctcctaa gagacagcct
     6241 tacgaaatag cagggctgcg tgttaaccac tccaccacgt tgtctattat tactaatcca
     6301 tgacgttaat tcaccatcta cagtcccacc ttggcagaag agggtgaaga gcattg
//
