LOCUS       sequence-17525-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Schalm SS, Blenis J
  TITLE     Identification of a conserved motif required for mTOR signaling.
  JOURNAL   Curr Biol. 2002 Apr 16. 12(8):632-9.
  PUBMED    11967149
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2002 Apr 16. 12(8):632-9."
COMMENT     Sequence Label: pRK7-HA-S6K1-KR
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        219..237
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     219..236
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     250..269
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             330..356
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
       61 cgatccagcc tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt
      121 gacgtaagta ccgcctatag agtctatagg cccaccccct tggcttcgtt agaacgcggc
      181 tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca ctatagaata
      241 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg cacctcggtt
      301 ctaagcttct gcaggtcgac tctagaatgt acccatacga tgttccagat tacgctgcag
      361 gagtgtttga catagacctg gaccagccag aggatgcagg ctctgaggat gagctggagg
      421 aggggggtca gttaaatgaa agcatggacc atgggggagt tggaccatat gaacttggca
      481 tggaacattg tgagaaattt gaaatctcag aaactagtgt gaacagaggg ccagaaaaaa
      541 tcagaccaga atgttttgag ctacttcggg tacttggtaa agggggctat ggaaaggttt
      601 ttcaagtacg aaaagtaaca ggagcaaata ctgggaagat atttgccatg agggtgctta
      661 aaaaggcaat gatagtaaga aatgctaaag atacagctca tacaa
//
