LOCUS       sequence-175253-        4706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human p300 amino acids 230-670 with 
            N-terminal GAL4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Askew EB, Bai S, Blackwelder AJ, Wilson EM
  TITLE     Transcriptional synergy between melanoma antigen gene protein-A11 
            (MAGE-11) and p300 in androgen receptor signaling.
  JOURNAL   J Biol Chem. 2010 Jul 9;285(28):21824-36. doi: 
            10.1074/jbc.M110.120600. Epub 2010 May 6.
  PUBMED    20448036
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.120600"; journalName: "J Biol Chem"; date: 
            "2010-07-9- 9"; volume: "285"; issue: "28"; pages: "21824-36"
COMMENT     Sequence Label: GAL-p300-230-670
FEATURES             Location/Qualifiers
     source          1..4706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..75)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             397..837
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    2330..2464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2367..2386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2421..2440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2527..2546)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2811..2828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2982..3570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3062..3081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3741..4532,4533..4601))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4364..4383
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4602..4706)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacct tctgaggcgg aaagaaccag ctgtggaatg
       61 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      121 tgcatctcaa ttagtcagca aggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgaag aggctttttt
      361 ggaggagatc taagcttgaa gcaagcctcc tgaaagatga agctactgtc ttctatcgaa
      421 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      481 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
      541 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
      601 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat
      661 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca
      721 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt
      781 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg
      841 gaattcccgg ggatccgtcg acatatggga ggacaaacag gattgagagg cccccagcct
      901 cttaagatgg gaatgatgaa caaccccaat ccttatggtt caccatatac tcagaatcct
      961 ggacagcaga ttggagccag tggccttggt ctccagattc agacaaaaac tgtactatca
     1021 aataacttat ctccatttgc tatggacaaa aaggcagttc ctggtggagg aatgcccaac
     1081 atgggtcaac agccagcccc gcaggtccag cagccaggtc tggtgactcc agttgcccaa
     1141 gggatgggtt ctggagcaca tacagctgat ccagagaagc gcaagctcat ccagcagcag
     1201 cttgttctcc ttttgcatgc tcacaagtgc cagcgccggg aacaggccaa tggggaagtg
     1261 aggcagtgca accttcccca ctgtcgcaca atgaagaatg tcctaaacca catgacacac
     1321 tgccagtcag gcaagtcttg ccaagtggca cactgtgcat cttctcgaca aatcatttca
     1381 cactggaaga attgtacaag acatgattgt cctgtgtgtc tccccctcaa aaatgctggt
     1441 gataagagaa atcaacagcc aattttgact ggagcacccg ttggacttgg aaatcctagc
     1501 tctctagggg tgggtcaaca gtctgccccc aacctaagca ctgttagtca gattgatccc
     1561 agctccatag aaagagccta tgcagctctt ggactaccct atcaagtaaa tcagatgccg
     1621 acacaacccc aggtgcaagc aaagaaccag cagaatcagc agcctgggca gtctccccaa
     1681 ggcatgcggc ccatgagcaa catgagtgct agtcctatgg gagtaaatgg aggtgtagga
     1741 gttcaaacgc cgagtcttct ttctgactca atgttgcatt cagccataaa ttctcaaaac
     1801 ccaatgatga gtgaaaatgc cagtgtgccc tccctgggtc ctatgccaac agcagctcaa
     1861 ccatccacta ctggaattcg gaaacagtgg cacgaagata ttactcagga tcttcgaaat
     1921 catcttgttc acaaactcgt ccaagccata tttcctacgc cggatcctgc tgctttaaaa
     1981 gacagacgga tggaaaacct agttgcatat gctcggaaag ttgaagggga catgtatgaa
     2041 tctgcaaaca atcgagcgga atactaccac cttctagctg agaaaatcta taagatccag
     2101 aaagaactag aagaaaaacg aaggaccaga ctacagaagc agaacatgct accaaatgct
     2161 gcaggcatgg ttccagtttc catgaattag atcgataatg catccgagct ctagataagt
     2221 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
     2281 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2341 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2401 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     2461 gatcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     2521 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     2581 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     2641 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     2701 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgatatgcg gtgtgaaata
     2761 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     2821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3421 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3781 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4021 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     4081 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4141 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4201 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4261 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4321 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     4381 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4441 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4501 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4561 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4621 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4681 aaaaataaac aaataggggt tccgcg
//
