LOCUS       sequence-175254-        4880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human p300 amino acids 501-999 with 
            N-terminal GAL4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Askew EB, Bai S, Blackwelder AJ, Wilson EM
  TITLE     Transcriptional synergy between melanoma antigen gene protein-A11 
            (MAGE-11) and p300 in androgen receptor signaling.
  JOURNAL   J Biol Chem. 2010 Jul 9;285(28):21824-36. doi: 
            10.1074/jbc.M110.120600. Epub 2010 May 6.
  PUBMED    20448036
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.120600"; journalName: "J Biol Chem"; date: 
            "2010-07-9- 9"; volume: "285"; issue: "28"; pages: "21824-36"
COMMENT     Sequence Label: GAL-p300-501-999
FEATURES             Location/Qualifiers
     source          1..4880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..75)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             397..837
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    2504..2638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2541..2560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2595..2614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2701..2720)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2985..3002)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3156..3744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3236..3255)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3915..4706,4707..4775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4538..4557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4776..4880)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacct tctgaggcgg aaagaaccag ctgtggaatg
       61 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      121 tgcatctcaa ttagtcagca aggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgaag aggctttttt
      361 ggaggagatc taagcttgaa gcaagcctcc tgaaagatga agctactgtc ttctatcgaa
      421 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      481 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
      541 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
      601 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat
      661 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca
      721 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt
      781 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg
      841 gaattcccgg ggatccgtcg acagcaaggc atgcggccca tgagcaacat gagtgctagt
      901 cctatgggag taaatggagg tgtaggagtt caaacgccga gtcttctttc tgactcaatg
      961 ttgcattcag ccataaattc tcaaaaccca atgatgagtg aaaatgccag tgtgccctcc
     1021 ctgggtccta tgccaacagc agctcaacca tccactactg gaattcggaa acagtggcac
     1081 gaagatatta ctcaggatct tcgaaatcat cttgttcaca aactcgtcca agccatattt
     1141 cctacgccgg atcctgctgc tttaaaagac agacggatgg aaaacctagt tgcatatgct
     1201 cggaaagttg aaggggacat gtatgaatct gcaaacaatc gagcggaata ctaccacctt
     1261 ctagctgaga aaatctataa gatccagaaa gaactagaag aaaaacgaag gaccagacta
     1321 cagaagcaga acatgctacc aaatgctgca ggcatggttc cagtttccat gaatccaggg
     1381 cctaacatgg gacagccgca accaggaatg acttctaatg gccctctacc tgacccaagt
     1441 atgatccgtg gcagtgtgcc aaaccagatg atgcctcgaa taactccaca atctggtttg
     1501 aatcaatttg gccagatgag catggcccag ccccctattg taccccggca aacccctcct
     1561 cttcagcacc atggacagtt ggctcaacct ggagctctca acccgcctat gggctatggg
     1621 cctcgtatgc aacagccttc caaccagggc cagttccttc ctcagactca gttcccatca
     1681 cagggaatga atgtaacaaa tatccctttg gctccgtcca gcggtcaagc tccagtgtct
     1741 caagcacaaa tgtctagttc ttcctgcccg gtgaactctc ctataatgcc tccagggtct
     1801 caggggagcc acattcactg tccccagctt cctcaaccag ctcttcatca gaattcaccc
     1861 tcgcctgtac ctagtcgtac ccccacccct caccatactc ccccaagcat aggggctcag
     1921 cagccaccag caacaacaat tccagcccct gttcctacac ctcctgccat gccacctggg
     1981 ccacagtccc aggctctaca tccccctcca aggcagacac ctacaccacc aacaacacaa
     2041 cttccccaac aagtgcagcc ttcacttcct gctgcacctt ctgctgacca gccccagcag
     2101 cagcctcgct cacagcagag cacagcagcg tctgttccta ccccaacagc accgctgctt
     2161 cctccgcagc ctgcaactcc actttcccag ccagctgtaa gcattgaagg acaggtatca
     2221 aatcctccat ctactagtag cacagaagtg aattctcagg ccattgctga gaagcagcct
     2281 tcccaggaag tgaagatgga ggccaaaatg gaagtggatc aaccagaacc agcagatact
     2341 cagccggagg atatttcaga gtagatcgat aatgcatccg agctctagat aagtaatgat
     2401 cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2461 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     2521 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2581 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcga
     2641 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     2701 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     2761 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     2821 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     2881 gcttcctaat gcaggagtcg cataagggag agcgtcgata tgcggtgtga aataccgcac
     2941 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     3001 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3061 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3121 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3181 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3241 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3301 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3361 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3421 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3481 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3541 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3601 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3661 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3721 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3781 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3841 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3901 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4021 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4201 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     4261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4501 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     4561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4861 aaacaaatag gggttccgcg
//
