LOCUS       sequence-175255-        8898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Pennacchio LA, Ahituv N, Moses AM, Prabhakar S, Nobrega MA, Shoukry 
            M, Minovitsky S, Dubchak I, Holt A, Lewis KD, Plajzer-Frick I, 
            Akiyama J, De Val S, Afzal V, Black BL, Couronne O, Eisen MB, Visel 
            A, Rubin EM
  TITLE     In vivo enhancer analysis of human conserved non-coding sequences.
  JOURNAL   Nature. 2006 Nov 23;444(7118):499-502. Epub 2006 Nov 5.
  PUBMED    17086198
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2006-11-23"; volume: "444(7118)"; pages: "499-502. Epub 2006 
            Nov 5"
COMMENT     Sequence Label: Hsp68-LacZ-Gateway
FEATURES             Location/Qualifiers
     source          1..8898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(77..382)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(179..198)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(724..1383)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1298..1317
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1437..1443,1444..1461,1462..1467))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(1492..1616)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1641..1657)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1642..1658)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1686..1704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1725..1741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1725..1741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1738..1760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1749..1765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1773..1779,1780..1797,1798..1803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1818..1839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1956..1973)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2127..2715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2207..2226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2886..3677,3678..3746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3509..3528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3747..3851)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3878..4333
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3965..3984)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4175..4196
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4473..4490
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4474..4490
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4497..4516
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4497..4515
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4551..4567
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4551..4567
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    4564..4698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4588..4607)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4642..4661
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4846..7893)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     protein_bind    join(8810..8898,1..36)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ataatactgt aaaacacaac atatccagtc actatggtcg acctgcagac tggctgtgta
       61 taagggagcc tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat
      121 tttcgcggtg gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca
      181 tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac
      241 gggagacttt atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg
      301 gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat
      361 aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcagcaaa agagccgttc
      421 atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg
      481 gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga gatattgaca
      541 tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct
      601 tcatagcata cctctttttg acatacttcg ggtatacata tcagtatata ttcttatacc
      661 gcaaaaatca gcgcgcaaat acgcatactg ttatctggct tttagtaagc cggatccacg
      721 cgtttacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
      781 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
      841 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
      901 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
      961 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     1021 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     1081 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     1141 ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat
     1201 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     1261 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     1321 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     1381 cattttagct tccttagctc ctgaaaatct cgacggatcc taactcaaaa tccacacatt
     1441 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgcggccg ccatagtgac
     1501 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     1561 atattgatat ttatatcatt ttacgtttct cgttcagctt ttttgtacaa acttgtgatg
     1621 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccagcttt
     1681 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     1741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3841 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     3901 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3961 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4021 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4081 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4141 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4201 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4261 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4321 acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     4381 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     4441 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     4501 acgactcact atagggcgaa ttggcgtcga cgcaccgcgg tggcggccgc tctagaacta
     4561 gtggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4621 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4681 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4741 gaggtgtggg aggtttttta aagcaagtaa aacctctaca gatgtgatat ggctgattat
     4801 gatcattact tatctagagc ttagatcccc cctgcccggt tattattatt tttgacacca
     4861 gaccaactgg taatggtagc gaccggcgct cagctggaat tccgccgata ctgacgggct
     4921 ccaggagtcg tcgccaccaa tccccatatg gaaaccgtcg atattcagcc atgtgccttc
     4981 ttccgcgtgc agcagatggc gatggctggt ttccatcagt tgctgttgac tgtagcggct
     5041 gatgttgaac tggaagtcgc cgcgccactg gtgtgggcca taattcaatt cgcgcgtccc
     5101 gcagcgcaga ccgttttcgc tcgggaagac gtacggggta tacatgtctg acaatggcag
     5161 atcccagcgg tcaaaacagg cggcagtaag gcggtcggga tagttttctt gcggccctaa
     5221 tccgagccag tttacccgct ctgctacctg cgccagctgg cagttcaggc caatccgcgc
     5281 cggatgcggt gtatcgctcg ccacttcaac atcaacggta atcgccattt gaccactacc
     5341 atcaatccgg taggttttcc ggctgataaa taaggttttc ccctgatgct gccacgcgtg
     5401 agcggtcgta atcagcaccg catcagcaag tgtatctgcc gtgcactgca acaacgctgc
     5461 ttcggcctgg taatggcccg ccgccttcca gcgttcgacc caggcgttag ggtcaatgcg
     5521 ggtcgcttca cttacgccaa tgtcgttatc cagcggtgca cgggtgaact gatcgcgcag
     5581 cggcgtcagc agttgttttt tatcgccaat ccacatctgt gaaagaaagc ctgactggcg
     5641 gttaaattgc caacgcttat tacccagctc gatgcaaaaa tccatttcgc tggtggtcag
     5701 atgcgggatg gcgtgggacg cggcggggag cgtcacactg aggttttccg ccagacgcca
     5761 ctgctgccag gcgctgatgt gcccggcttc tgaccatgcg gtcgcgttcg gttgcactac
     5821 gcgtactgtg agccagagtt gcccggcgct ctccggctgc ggtagttcag gcagttcaat
     5881 caactgttta ccttgtggag cgacatccag aggcacttca ccgcttgcca gcggcttacc
     5941 atccagcgcc accatccagt gcaggagctc gttatcgcta tgacggaaca ggtattcgct
     6001 ggtcacttcg atggtttgcc cggataaacg gaactggaaa aactgctgct ggtgttttgc
     6061 ttccgtcagc gctggatgcg gcgtgcggtc ggcaaagacc agaccgttca tacagaactg
     6121 gcgatcgttc ggcgtatcgc caaaatcacc gccgtaagcc gaccacgggt tgccgttttc
     6181 atcatattta atcagcgact gatccaccca gtcccagacg aagccgccct gtaaacgggg
     6241 atactgacga aacgcctgcc agtatttagc gaaaccgcca agactgttac ccatcgcgtg
     6301 ggcgtattcg caaaggatca gcgggcgcgt ctctccaggt agcgaaagcc attttttgat
     6361 ggaccatttc ggcacagccg ggaagggctg gtcttcatcc acgcgcgcgt acatcgggca
     6421 aataatatcg gtggccgtgg tgtcggctcc gccgccttca tactgcaccg ggcgggaagg
     6481 atcgacagat ttgatccagc gatacagcgc gtcgtgatta gcgccgtggc ctgattcatt
     6541 ccccagcgac cagatgatca cactcgggtg attacgatcg cgctgcacca ttcgcgttac
     6601 gcgttcgctc atcgccggta gccagcgcgg atcatcggtc agacgattca ttggcaccat
     6661 gccgtgggtt tcaatattgg cttcatccac cacatacagg ccgtagcggt cgcacagcgt
     6721 gtaccacagc ggatggttcg gataatgcga acagcgcacg gcgttaaagt tgttctgctt
     6781 catcagcagg atatcctgca ccatcgtctg ctcatccatg acctgaccat gcagaggatg
     6841 atgctcgtga cggttaacgc ctcgaatcag caacggcttg ccgttcagca gcagcagacc
     6901 attttcaatc cgcacctcgc ggaaaccgac atcgcaggct tctgcttcaa tcagcgtgcc
     6961 gtcggcggtg tgcagttcaa ccaccgcacg atagagattc gggatttcgg cgctccacag
     7021 tttcgggttt tcgacgttca gacgtagtgt gacgcgatcg gcataaccac cacgctcatc
     7081 gataatttca ccgccgaaag gcgcggtgcc gctggcgacc tgcgtttcac cctgccataa
     7141 agaaactgtt acccgtaggt agtcacgcaa ctcgccgcac atctgaactt cagcctccag
     7201 tacagcgcgg ctgaaatcat cattaaagcg agtggcaaca tggaaatcgc tgatttgtgt
     7261 agtcggttta tgcagcaacg agacgtcacg gaaaatgccg ctcatccgcc acatatcctg
     7321 atcttccaga taactgccgt cactccagcg cagcaccatc accgcgaggc ggttttctcc
     7381 ggcgcgtaaa aatgcgctca ggtcaaattc agacggcaaa cgactgtcct ggccgtaacc
     7441 gacccagcgc ccgttgcacc acagatgaaa cgccgagtta acgccatcaa aaataattcg
     7501 cgtctggcct tcctgtagcc agctttcatc aacattaaat gtgagcgagt aacaacccgt
     7561 cggattctcc gtgggaacaa acggcggatt gaccgtaatg ggataggtca cgttggtgta
     7621 gatgggcgca tcgtaaccgt gcatctgcca gtttgagggg acgacgacag tatcggcctc
     7681 aggaagatcg cactccagcc agctttccgg caccgcttct ggtgccggaa accaggcaaa
     7741 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     7801 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     7861 agggttttcc cagtcacgac gttgtaaaac gacgggatca tcgcgagcca tggcgccgcg
     7921 ctctgcttct ggaaggctgc gctccgcggc gtggatgctc cggggaaagt tgcgctccgc
     7981 ggcagggatg ctcctgggaa ggttgcgctc cgcggcaggg atgctctggg gaaggctggt
     8041 cctggccgag gatcgggaac gcgccgctcg ctctgcttct cttgtcttcg cttgtctctg
     8101 gatggaacca gatttggttc tgagtagctg tcagcgtctg gtgacctgct cgccgccctg
     8161 cgcctttaag gagtcttcac cggccccgcc cactctccgc tgggccaatc agcgagccgg
     8221 aggaggcctt ggggccagga atcttccagc agtttcgcgt ctggtggagc ttccccgcct
     8281 cccttgagta atcggagttg tgggttccgc ccttgtccag aactctccag aggtttctgg
     8341 ggttcactgg agagtacgga ttcctgaggg ggagggtgtg gggaagtgct ggtgctacta
     8401 gtgacactgt tgctatggcg acgcattact aaggcctgtg tggaatggac aagaaagatc
     8461 acctctagct cggtgttgtg tacagtttgt tgtgatttgt ggggtttcgc caactcgcac
     8521 agttctgaat atgggggtta aaggctaaaa cttaagggct aaaacttctc cccgccaagt
     8581 ttaggagacc cagggagatg cctgggggcg tgtccggtga cgtgatcctc tccaatcgcg
     8641 ttacaatggc agtgctgcct ctgacctcat ggactaattt aggaactaga ggctctgtcc
     8701 cagcacaggc tcaaagttgc cgggaggggc ggggtggggg gtggggggga ccccggctgc
     8761 tcagtttgga tgttcctgga gctcggtacc cggggatccc ccgggcatca ccactttgta
     8821 caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     8881 gcataaaaaa cagactac
//
