LOCUS       sequence-175262-        4925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human p300 amino acids 1901-2414 with 
            N-terminal GAL4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Askew EB, Bai S, Blackwelder AJ, Wilson EM
  TITLE     Transcriptional synergy between melanoma antigen gene protein-A11 
            (MAGE-11) and p300 in androgen receptor signaling.
  JOURNAL   J Biol Chem. 2010 Jul 9;285(28):21824-36. doi: 
            10.1074/jbc.M110.120600. Epub 2010 May 6.
  PUBMED    20448036
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.120600"; journalName: "J Biol Chem"; date: 
            "2010-07-9- 9"; volume: "285"; issue: "28"; pages: "21824-36"
COMMENT     Sequence Label: GAL-p300-1901-2414
FEATURES             Location/Qualifiers
     source          1..4925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..75)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             397..837
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    2549..2683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2586..2605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2640..2659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2746..2765)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3030..3047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3201..3789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3281..3300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3960..4751,4752..4820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4583..4602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4821..4925)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacct tctgaggcgg aaagaaccag ctgtggaatg
       61 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      121 tgcatctcaa ttagtcagca aggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgaag aggctttttt
      361 ggaggagatc taagcttgaa gcaagcctcc tgaaagatga agctactgtc ttctatcgaa
      421 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      481 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
      541 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
      601 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat
      661 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca
      721 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt
      781 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg
      841 gaattcccgg ggatccgtcg acaggcagca ggccaggtga cccctccaac ccctcctcag
      901 actgctcagc caccccttcc agggccccca cctgcagcag tggaaatggc aatgcagatt
      961 cagagagcag cggagacgca gcgccagatg gcccacgtgc aaatttttca aaggccaatc
     1021 caacaccaga tgcccccgat gactcccatg gcccccatgg gtatgaaccc acctcccatg
     1081 accagaggtc ccagtgggca tttggagcca gggatgggac cgacagggat gcagcaacag
     1141 ccaccctgga gccaaggagg attgcctcag ccccagcaac tacagtctgg gatgccaagg
     1201 ccagccatga tgtcagtggc ccagcatggt caacctttga acatggctcc acaaccagga
     1261 ttgggccagg taggtatcag cccactcaaa ccaggcactg tgtctcaaca agccttacaa
     1321 aaccttttgc ggactctcag gtctcccagc tctcccctgc agcagcaaca ggtgcttagt
     1381 atccttcacg ccaaccccca gctgttggct gcattcatca agcagcgggc tgccaagtat
     1441 gccaactcta atccacaacc catccctggg cagcctggca tgccccaggg gcagccaggg
     1501 ctacagccac ctaccatgcc aggtcagcag ggggtccact ccaatccagc catgcagaac
     1561 atgaatccaa tgcaggcggg cgttcagagg gctggcctgc cccagcagca accacagcag
     1621 caactccagc cacccatggg agggatgagc ccccaggctc agcagatgaa catgaaccac
     1681 aacaccatgc cttcacaatt ccgagacatc ttgagacgac agcaaatgat gcaacagcag
     1741 cagcaacagg gagcagggcc aggaataggc cctggaatgg ccaaccataa ccagttccag
     1801 caaccccaag gagttggcta cccaccacag cagcagcagc ggatgcagca tcacatgcaa
     1861 cagatgcaac aaggaaatat gggacagata ggccagcttc cccaggcctt gggagcagag
     1921 gcaggtgcca gtctacaggc ctatcagcag cgactccttc agcaacagat ggggtcccct
     1981 gttcagccca accccatgag cccccagcag catatgctcc caaatcaggc ccagtcccca
     2041 cacctacaag gccagcagat ccctaattct ctctccaatc aagtgcgctc tccccagcct
     2101 gtcccttctc cacggccaca gtcccagccc ccccactcca gtccttcccc aaggatgcag
     2161 cctcagcctt ctccacacca cgtttcccca cagacaagtt ccccacatcc tggactggta
     2221 gctgcccagg ccaaccccat ggaacaaggg cattttgcca gcccggacca gaattcaatg
     2281 ctttctcagc ttgctagcaa tccaggcatg gcaaacctcc atggtgcaag cgccacggac
     2341 ctgggactca gcaccgataa ctcagacttg aattcaaacc tctcacagag tacactagac
     2401 atacactaga tcgataatgc atccgagctc tagataagta atgatcataa tcagccatat
     2461 cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2521 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2581 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2641 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcgatcctc tacgccggac
     2701 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     2761 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     2821 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     2881 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     2941 agtcgcataa gggagagcgt cgatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     3001 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3061 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3601 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     3661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4261 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4561 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4921 ccgcg
//
