LOCUS       sequence-175275-       11599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - 
            EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - IRES - MTS - tFD - P2A 
            - MTS - tFNR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, 
            Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and 
            Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded 
            PhyB Optogenetics.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 
            10.1021/acssynbio.7b00413. Epub 2018 Jan 24.
  PUBMED    29301067
REFERENCE   2  (bases 1 to 11599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00413"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "706-717"
COMMENT     Sequence Label: pPKm-244
FEATURES             Location/Qualifiers
     source          1..11599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1264..1338
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      1360..1369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1369..1398
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2137..2193
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2194..2268
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2296..2319
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2760..2769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(3058..3632,3633..3635,3636..3644)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3225..3242)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3452..3471
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3651..3725
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      3747..3756
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3756..3797
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4107..4163
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4164..4238
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             4269..4286
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5479..5499)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5484..5813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5664..5799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5823..6422
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(5823..5842)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6319..6339
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6715..6948
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7099..7116)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7105..7329
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      7372..7381
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7425..7529
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7530..7598,7599..8390)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7748..7767)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8561..9149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9050..9069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             9308..9941
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9988..10113
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10606..10839
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11024..11068
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11355..11472
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc ttcgaactag
     1201 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agttcgaagg atccgccgcc
     1261 accatgtccg tgctgacccc actcctgctt aggggcctta ccggctctgc caggcgcctc
     1321 ccggtcccac gggcaaagat ccactcattg ttcgaagccg ccaccatgga acagaagctg
     1381 atctccgaag aggacctgat gggcacagcc accatgacaa ccagtttggc tacaaagttg
     1441 agagagggta ctaaaaaggc tcacaccatg gcagagaacg tggggttcgt gcggtgtttt
     1501 ctgaagggga cagttgagaa aagctcttat aggaaactgg tcgcgtcctt gtaccatgtc
     1561 tattccgcga tggagcaaga gatggagaga ttgaaggatc acccaatcgt tgggaagatc
     1621 tattttcctg agctgaatcg gaagagtagt ctggagaggg atttgaccta ttactttggt
     1681 agcaattggc gcgaagagat cccaccctct ccagccactc aggcttacgt ggcccgaatc
     1741 catgaggtgg caaacactgc ccccgaactg ctggtagcac attcatatac tcgatatctt
     1801 ggggatctct ccggaggtca gattcttaaa ggcatcgctg agcgagcaat gaatctgcaa
     1861 gatggagagg gcactgcttt ctatcggttt gagagcatct ctgacgagaa agcctttaaa
     1921 cagctgtatc gacagcggct cgacgaactg cctgtcgacg aggccaccgc agaccgcatc
     1981 gtcgacgagg ctaatgctgc tttcggaatg aacatgaaga tctttcagga gctggaaggg
     2041 aatctgatcc gggctatcgg tcaactgctc tttaacactc tgaccaggcg gaagcagagg
     2101 gggtctactg agcttgctac agccgatgga tctggggcca caaatttttc cctgctgaaa
     2161 caggctgggg acgtggagga aaatccaggc cccatgagcg ttctcacacc tttgctgctc
     2221 cgggggctca caggcagtgc tcgacgactg ccagtcccga gggcgaagat tcactcactg
     2281 gctagcggat caatggatta caaggatgac gacgataagt ctagcatggg gactgcaact
     2341 atgtctctga ggcaacatca acatcctctc atccagagac tcgcagatag aattgaggca
     2401 atttggcagg cctttttccc actggctcca tacgcactgc cggaggactt gggctacgtg
     2461 gaagggaagc ttgaaggcga gaggttgact attgaaaatc actgttatca ggccccgcct
     2521 tttagaaaac tgcatttgga gttggcgagg gtcggtgagt ctctggatat tttgcactgt
     2581 gtcatgtttc ccgaacctcg gtacgacctt cccatgttcg gctgcgatct ggtgggaggc
     2641 cggggccaaa tctctgccgc aattgttgac ctctcaccag tgaccggaca gttgccagct
     2701 gcctacacct gtgctttgaa cgcactgcct aagctgacat ttagacagcc ccgagagctg
     2761 ccaccatggg gacacatttt ttcacccttc tgcattttta ttaggcccca gggcgaggct
     2821 gaggagcagc agtttttgga tagaatcggt gaatatctta ctctgcactg ccagttgtcc
     2881 caacaggctg tgccaaccga ccaccctcaa gcagtcattg ctggccagcg gcagtattgt
     2941 cagcagcagc agcaaaacga taagacaagg agggtactgg agaaggcatt tggtgtaccc
     3001 tgggccgagc ggtatatgac tactgttctg ttcgacgttc cacccgtgta acatatgccc
     3061 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     3121 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     3181 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3241 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3301 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3361 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3421 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3481 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac
     3541 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     3601 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccactagt atgagcgtgc
     3661 tgacgccctt gctgcttaga ggactgaccg gaagcgcacg ccgcctgccc gtgcctaggg
     3721 cgaagataca tagcctgttc gaagccgcca ccatgggtaa gcccattccc aacccgctgt
     3781 tgggcctcga tagcacagtt aacatggcta cctacaaagt gacactggtt cggcctgatg
     3841 gtagcgagac aacaattgac gtgcctgagg acgaatacat actcgacgtt gcggaagagc
     3901 aagggctgga tctgccattt agttgccgag cgggcgcttg cagcacttgt gccgggaagt
     3961 tgctcgaagg ggaagtggat cagtctgatc agtcctttct ggatgacgat cagatcgaaa
     4021 aagggttcgt gctcacctgt gtcgcttatc caaggagcga ttgtaagatt ctcactaatc
     4081 aggaggagga gctttacggc tcaggcgcga caaatttttc tctcctcaaa caggctgggg
     4141 acgtggagga gaaccctggc cctatgtccg ttcttacccc actgttgttg cggggactca
     4201 ctggatcggc tagacgactc cccgtcccaa gggcaaagat ccattctctg gaattcgccg
     4261 ccaccatgca ccaccaccat caccacgtta acatgtacaa tgcaaccaac tccaggagca
     4321 gaatgtttag atatgaagta gtaggactga ggcagactgc cgaaaccgaa aagaccaact
     4381 atgccatacg aaatagcgga agccagtttt ttaacgtacc ctatgatagg atgaatcagt
     4441 tcatgcagca gataacacgc tggggcggaa agatcgtatc cattcagccc ttgaacggca
     4501 cagtggctcc cctggctgcc accacggaac ccgctgcaaa taatggcgct gcgcccgtca
     4561 aagagaaaaa ggttgatata ccagttaaca tctatcgccc gaacaacccc tgtattggaa
     4621 aagtaatatc caacgaggaa ctggtgaggg agggcggtga gggaaccgtg aaacacatta
     4681 tttttgatat ctctggaacc gagctcagat atttggaggg ccagtctatc gggatcattc
     4741 cggctgggac cgacgcaaat ggtaaaccac ataaactccg gctgtattct atcgcctcaa
     4801 cgaggcacgg ggactttcag gatgacaaga ccgtctcact gtgcgttaga cgcctcgaat
     4861 ataaagataa agagactggc gagaccattt atggcgtgtg ctcctcctac ctgaatcagc
     4921 tgcagcctgg agacgaagtg aagattactg gtcccgttgg gaaggaaatg ctgttgagtg
     4981 acgaccctga agccaccatc ataatgcttg ctaccggcac cggaattgcc ccctttcgcg
     5041 ctttcttgtg gcggatgttc aaagaaaaca acccagatta tcagttcaaa ggacttgcct
     5101 ggctgttttt tggcgtcgcc tacaccgcta acatcctgta taaagatgag ctggaggcca
     5161 tccaggccca gtatcctgat cactttcggc ttacatacgc aatttcacga gagcagaaga
     5221 ctccggacgg tgggaaaatg tacatccagg ggcgaatcgc cgaacatgct gacgagattt
     5281 ggcagttgct tcagaaaaaa aacacacacg tctatatgtg cggcctgagg ggcatggaac
     5341 ctgggataga tgaagcaatg acagcagccg ccgccaagaa tggcgcagat tggcaggaat
     5401 ttcttaaagg aacattgaag aaagagggac ggtggcacgt ggaaacatat taataaccag
     5461 cacagtggtc gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5641 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5761 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta
     5821 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5881 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5941 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     6001 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     6061 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     6121 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     6181 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     6241 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     6301 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     6361 ccgacgtcga gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag cccggtgcct
     6421 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga
     6481 taaaataaaa gattttattt agtctccaga aaaagggggc ggccgctcga gacctagaaa
     6541 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     6601 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6661 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6721 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6781 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6841 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6901 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     6961 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     7021 cttgacatta taatagattt agcaggaatt gaactaggag tggagcacac aggcaaagtt
     7081 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     7141 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     7201 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7261 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7321 gctctatggc ttctgaggcg gaaagaacca gctgggggcg cgcccctcga ggccgccatg
     7381 gtcatagctg tttgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7441 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7501 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7561 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7621 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7681 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7741 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7801 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7861 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7921 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7981 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8041 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8101 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8161 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8221 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8281 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8341 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8401 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8461 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8701 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8941 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9121 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9181 tggccttttg ccggaccgcc actgccaatt acctgtggtt tcatttactc taaacctgtg
     9241 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     9301 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     9361 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     9421 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     9481 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     9541 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     9601 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     9661 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     9721 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     9781 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     9841 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     9901 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     9961 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    10021 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    10081 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    10141 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    10201 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    10261 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    10321 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    10381 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    10441 gcacagcaac cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    10501 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    10561 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    10621 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    10681 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    10741 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    10801 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    10861 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    10921 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    10981 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    11041 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    11101 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    11161 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    11221 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    11281 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc acaaatggca
    11341 gtattcatcc acaattttaa aagaaagggg gggattgggg ggtacagtgc aggggaaaga
    11401 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    11461 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggtc gataagcttt
    11521 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
    11581 cttgaaagga gtgggaatt
//
