LOCUS       sequence-175296-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express MORF4L1 (iso2) with a FLAG tag at N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..133
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
       61 ggaccgatcc agcctccgga ctctagcgtt taaacttaag gccgccatgg attacaagga
      121 tgatgatgat aagcttatgg cgccgaagca ggacccgaag cctaaattcc aggagggtga
      181 gcgagtgctg tgctttcatg ggcctcttct ttatgaagca aagtgtgtaa aggttgccat
      241 aaaggacaaa caagtgaaat acttcataca ttacagtggt tggaataaaa attgggatga
      301 atgggttccg gagagcagag tactcaaata cgtggacacc aatttgcaga aacagcgaga
      361 acttcaaaaa gccaatcagg agcagtatgc agaggggaag atgagagggg ctgccccagg
      421 aaagaagaca tctggtctgc aacagaaaaa tgttgaagtg aaaacgaaaa agaacaaaca
      481 gaaaacacct ggaaatggag atggtggcag taccagtgag acccctcagc ctcctcggaa
      541 gaaaagggcc cgggtagatc ctactgttga aaatgaggaa acattcatga acagagttga
      601 agttaaagta aagattcctg aagagctaaa accgtggctt gttgatgact gggacttaat
      661 taccaggcaa aaacagctct tttatcttcc tgccaagaag aatgtggatt ccattcttga
      721 ggattatgca aattacaaga aatctcgtgg aaacacagat aataaggagt atgcggttaa
      781 tgaagttgtg gcagggataa aagaatactt caacgtaatg ttgggtaccc agctactcta
      841 taaatttgag agaccacagt atgctgaaat tcttgcagat catcccgatg cacccatgtc
      901 ccaggtgtat ggagcgccac atctcctgag attatttgta cgaattggag caatgttggc
      961 ttatacacct ctggatgaga agagccttgc tttattactc aattatcttc acgatttcct
     1021 aaagtacctg gcaaagaatt ctgcaacttt gttcagtgcc agcgattatg aagtggctcc
     1081 tcctgagtac catcggaaag ctgtgtgagg
//
