LOCUS       sequence-175299-        8239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) 
            with a Myc and HA tag at the N- and C-terminue, respectively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Kedinger V, Sansregret L, Harada R, Vadnais C, Cadieux C, Fathers K,
            Park M, Nepveu A
  TITLE     p110 CUX1 homeodomain protein stimulates cell migration and invasion
            in part through a regulatory cascade culminating in the repression 
            of E-cadherin and occludin.
  JOURNAL   J Biol Chem. 2009 Oct 2;284(40):27701-11. doi: 
            10.1074/jbc.M109.031849. Epub 2009 Jul 27.
  PUBMED    19635798
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.031849"; journalName: "J Biol Chem"; date: 
            "2009-10-2- 2"; volume: "284"; issue: "40"; pages: "27701-11"
COMMENT     Sequence Label: pLXSN p110 CUX1
FEATURES             Location/Qualifiers
     source          1..8239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             588..763
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    826..1025
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1226..1642
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1572..1594
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1610..1626
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1674..1703
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3981..4007
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4037..4057)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4042..4371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4222..4357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4431..5225
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4485..4504)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5095..5114
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5285..5878
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5981..6003)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6089..6229
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6144..6163
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6244..6261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6415..7003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6495..6514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7174..7965,7966..8034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7797..7816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8035..8139)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8207..8225
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
       61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
      121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
      181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
      241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
      301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
      361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
      421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
      481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
      541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
      601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
      661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
      721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
      781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
      841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
      901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
      961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     1621 cctcactcct tctctaggcg ccggaattcg ttaactcgac tggatccacc atggagcaga
     1681 agctcatctc tgaagaggac ctctccacct cgcccatgcc caccgtgtcc agctacccac
     1741 ctctcgccat ctccctgaag aagccctccg cagctcctga ggccggtgcc tctgctctgc
     1801 cgaacccccc ggccctcaaa aaggaggccc aggacgcccc cgggctggac ccccagggag
     1861 cagccgattg tgcacaaggg gtcctgagac aggtgaaaaa tgaggtgggc cgcagcggtg
     1921 cctggaagga ccactggtgg agcgcggtgc agccggagag aagaaatgcc gcctcctccg
     1981 aggaggccaa ggccagagaa acgggcggcg ggaaagagaa gggcagcggt ggcagcggag
     2041 gtggcagcca gcctcgggcc gagcgcagtc agctccaggg accctcgtcg tcagagtact
     2101 ggaaggagtg gcccagcgct gagtccccat actcccagag ctcagagctg agtctgaccg
     2161 gggccagccg cagcgagaca ccacagaaca gccccctgcc atcctccccg atcgtgccca
     2221 tgtccaagcc caccaagccc tcggtccccc cgctgacccc cgagcagtac gaggtctaca
     2281 tgtaccagga ggtggacacc atcgagctca cccggcaggt taaggaaaag ctggccaaga
     2341 acggcatctg ccagagaatc ttcggggaga aggtgctggg cctgtcccag ggcagcgtca
     2401 gcgacatgct gtcccgaccg aagccatgga gcaagctgac gcagaaaggc cgagaaccct
     2461 tcatccggat gcagctctgg ctgaacggcg agctaggcca gggtgttcta cccgtccagg
     2521 gccagcagca agggccagtc ctccactccg tgacatcgct ccaggacccg ctgcagcagg
     2581 gctgtgtgag ctcagaaagc actccaaaga cctccgccag ctgcagccct gcccctgagt
     2641 ccccgatgag ttccagtgag tcggtgaaga gcctgaccga gctggtccag cagccctgtc
     2701 cccccatcga ggcgagcaag gacagcaagc caccagagcc cagtgacccg ccagcatccg
     2761 actcccagcc cacaaccccg ctgcctctct ccggacactc ggccctcagc atccaagaat
     2821 tagtagccat gtccccggag ctggacacct acggcataac caagcgggtg aaggaggtgc
     2881 tgacggacaa caacctcggc cagcgcttat ttggggagac catcttaggg ctcacccaag
     2941 gctctgtctc tgacctcctt gcccgcccca aaccctggca taagctcagt ctgaaaggac
     3001 gagagccctt cgtccggatg cagctgtggc tgaacgaccc caacaatgtg gagaagctga
     3061 tggacatgaa acggatggag aagaaagcct acatgaagcg gcggcacagc tcagtcagtg
     3121 acagccagcc ctgcgaaccg ccctctgtcg gcaccgagta cagccagggc gccagccccc
     3181 agccccagca ccagctgaag aaaccccggg tggtgctggc tccggaggag aaggaggcgc
     3241 tgaaacgagc gtatcagcaa aagccatacc cgtcaccaaa aaccatcgaa gacctcgcca
     3301 cccagctcaa cctgaaaaca agcaccgtca tcaactggtt ccacaactac aggtctcgga
     3361 tccgcagaga actgttcatt gaggaaattc aggccgggag tcagggccag gcgggcgcca
     3421 gcgactcacc ctcggcccgc agcggccggg cggcgcccag ctcggagggc gacagctgcg
     3481 acggcgtgga ggccactgag ggcccaggca gcgccgacac cgaggagccc aagtctcagg
     3541 gagaggccga gcgggaggag gtgccgcggc cggcggagca gacggagccg ccgccctcgg
     3601 ggaccccggg cccggacgac gcccgcgacg acgaccacga gggaggcccc gtggaaggcc
     3661 cggggcccct gcccagcccc gcctccgcga ccgccaccgc cgcgcccgcg gcccccgagg
     3721 acgccgctac ctcagccgcc gccgcgccgg gggagggccc cgcggccccg acgtccgcgc
     3781 caccgcccag caacagcagc agcagcagcg ccccccgcag gcccagctcg ctgcagagcc
     3841 ttttcggcct ccccgaggcc gcgggcgccc gggactcgcg cgacaacccc ctgcgcaaga
     3901 agaaggccgc gaacttgaac agcatcatcc accgcctgga gaaggccgcc agccgggagg
     3961 aacctatcga atgggagttc tatccatacg acgtacctga ctacgcatga gcggccgcac
     4021 tcgaggatcc ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4141 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4201 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4261 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4321 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttggg
     4381 ctgcaggtcg aggcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4441 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4501 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4561 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     4621 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4681 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4741 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4801 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4861 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4921 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     4981 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5041 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5101 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5161 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5221 tctgagcggg actctggggt tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     5281 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     5341 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     5401 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     5461 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     5521 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     5581 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     5641 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     5701 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     5761 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     5821 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
     5881 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt
     5941 aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     6001 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     6061 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     6121 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     6181 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     6241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6841 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7141 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7201 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7261 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7321 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7381 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7441 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     7501 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7561 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7621 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7681 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7741 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     7801 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7861 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7921 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7981 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8041 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8101 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8161 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     8221 gaggcccttt cgtcttcaa
//
