LOCUS       sequence-175300-        1141 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express CHK1 with a FLAG tag at N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pcDNA5/FRT/TO-FLAG-CHK1
FEATURES             Location/Qualifiers
     source          1..1141
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    30..48
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     58..82
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      170..179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             179..202
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
       61 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      121 aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagg ccgccatgga
      181 ttacaaggat gatgatgata agcttggtac catggcagtg ccctttgtgg aagactggga
      241 cttggtgcaa accctgggag aaggtgccta tggagaagtt caacttgctg tgaatagagt
      301 aactgaagaa gcagtcgcag tgaagattgt agatatgaag cgtgccgtag actgtccaga
      361 aaatattaag aaagagatct gtatcaataa aatgctaaat catgaaaatg tagtaaaatt
      421 ctatggtcac aggagagaag gcaatatcca atatttattt ctggagtact gtagtggagg
      481 agagcttttt gacagaatag agccagacat aggcatgcct gaaccagatg ctcagagatt
      541 cttccatcaa ctcatggcag gggtggttta tctgcatggt attggaataa ctcacaggga
      601 tattaaacca gaaaatcttc tgttggatga aagggataac ctcaaaatct cagactttgg
      661 cttggcaaca gtatttcggt ataataatcg tgagcgtttg ttgaacaaga tgtgtggtac
      721 tttaccatat gttgctccag aacttctgaa gagaagagaa tttcatgcag aaccagttga
      781 tgtttggtcc tgtggaatag tacttactgc aatgctcgct ggagaattgc catgggacca
      841 acccagtgac agctgtcagg agtattctga ctggaaagaa aaaaaaacat acctcaaccc
      901 ttggaaaaaa atcgattctg ctcctctagc tctgctgcat aaaatcttag ttgagaatcc
      961 atcagcaaga attaccattc cagacatcaa aaaagataga tggtacaaca aacccctcaa
     1021 gaaaggggca aaaaggcccc gagtcacttc aggtggtgtg tcagagtctc ccagtggatt
     1081 ttctaagcac attcaatcca atttggactt ctctccagta aacagtgctt ctagtgaaga
     1141 a
//
