LOCUS       sequence-175301-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express CHK1 with a FLAG tag at N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pcDNA5/FRT/TO-FLAG-CHK1
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aatcttctgt tggatgaaag ggataacctc aaaatctcag actttggctt ggcaacagta
       61 tttcggtata ataatcgtga gcgtttgttg aacaagatgt gtggtacttt accatatgtt
      121 gctccagaac ttctgaagag aagagaattt catgcagaac cagttgatgt ttggtcctgt
      181 ggaatagtac ttactgcaat gctcgctgga gaattgccat gggaccaacc cagtgacagc
      241 tgtcaggagt attctgactg gaaagaaaaa aaaacatacc tcaacccttg gaaaaaaatc
      301 gattctgctc ctctagctct gctgcataaa atcttagttg agaatccatc agcaagaatt
      361 accattccag acatcaaaaa agatagatgg tacaacaaac ccctcaagaa aggggcaaaa
      421 aggccccgag tcacttcagg tggtgtgtca gagtctccca gtggattttc taagcacatt
      481 caatccaatt tggacttctc tccagtaaac agtgcttcta gtgaagaaaa tgtgaagtac
      541 tccagttctc agccagaacc ccgcacaggt ctttccttat gggataccag cccctcatac
      601 attgataaat tggtacaagg gatcagcttt tcccagccca catgtcctga tcatatgctt
      661 ttgaatagtc agttacttgg caccccagga tcctcacaga acccctggca gcggttggtc
      721 aaaagaatga cacgattctt taccaaattg gatgcagaca aatcttatca atgcctgaaa
      781 gagacttgtg agaagttggg ctatcaatgg aagaaaagtt gtatgaatca ggttactata
      841 tcaacaactg ataggagaaa caataaactc attttcaaag tgaatttgtt agaaatggat
      901 gataaaatat tggttgactt ccggctttct aagggtgatg gattggagtt caagagacac
      961 ttcctgaaga ttaaagggaa gctgattgat attgtgagca gccagaag
//
