LOCUS       sequence-175303-        3743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab23 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(601..619)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(602..619)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(637..653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(637..653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(650..672)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    661..677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(685..691,692..709,710..715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    730..751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(868..885)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1039..1627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1119..1138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1798..2589,2590..2658))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2421..2440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2659..2763)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2841..3296)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2978..2999)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3190..3209
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3422..3444
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3436..3453
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3437..3453
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3460..3479
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3460..3478
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3509..3518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3513..3522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggaggagttt gatgccatca ctaaagctta ttacagagga gctcaggcct gtgtgctggt
       61 tttctccacc accgacagag agtcattcga ggccatcagc agctggaagg agaaggtgga
      121 gatggaggtg ggcgacattc ccactgtcct ggtgcagaac aaaatcgacc tgctggacga
      181 caccgtcatc aagaatgaag aggctgaagg tcttgctaaa aggctgaagt tgagattcta
      241 ccggacgtca gtgaaggagg atctgaatgt caatgaagtt tttaagtatc tagctgacaa
      301 atatctgcag cgactcaagc agcagtcagc ggaggagacg gatatcattc acacatccag
      361 caataaaata ggcgtcttca acacaacagg cagcggtcat ccaaaccaga acagcagcga
      421 tttgaatggc cgagagatcg taaacctgcg accaaacaag cagcggacga agaaaacgaa
      481 aaaccccttc ggcagctgca gactcctgta gagccccaca aatcactagt gaattcgcgg
      541 ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat
      601 tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      661 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      721 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      781 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      841 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      901 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      961 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1021 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1081 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1141 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1201 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1261 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1321 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1381 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1441 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1501 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1561 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1621 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1681 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1741 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1801 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1861 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1921 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     1981 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2041 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2101 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2161 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2221 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2281 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2341 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2401 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2461 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2521 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     2581 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     2641 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     2701 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     2761 gcgcacattt ccccgaaaag tgccacctga tgcggtgtga aataccgcac agatgcgtaa
     2821 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     2881 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     2941 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3001 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3061 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     3121 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3181 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     3241 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     3301 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     3361 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     3421 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc
     3481 gaattgggcc cgacgtcgca tgctcccggc cgccatggcg gccgcgggaa ttcgattgtc
     3541 atgctggagg aggacatgga ggtggccatc aaggtggtgg tggtgggaaa cggagcggtg
     3601 ggcaagtcca gcatgatcca gaggtactgc aagggcatct tcaccaagga ctacaagaag
     3661 accatcggcg tggactttct ggagaggcag atcatagtga acggcgagga cgtcagactg
     3721 atgttgtggg acacggcggg gca
//
