LOCUS       sequence-175322-        6457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of full-length human MAGE-A11 with N-terminal 
            FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Bai S, He B, Wilson EM
  TITLE     Melanoma antigen gene protein MAGE-11 regulates androgen receptor 
            function by modulating the interdomain interaction.
  JOURNAL   Mol Cell Biol. 2005 Feb;25(4):1238-57.
  PUBMED    15684378
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-02"; volume: "25(4)"; pages: "1238-57"
COMMENT     Sequence Label: pCMV-FLAG-MAGE-2-429
FEATURES             Location/Qualifiers
     source          1..6457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    44..666
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(243..262)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(690..710)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        695..1024
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      875..1010
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1062..1081)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1063..1081)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1095..1111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1095..1111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1108..1130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1119..1135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1143..1149,1150..1167,1168..1173))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1188..1209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1326..1343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1497..2085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1577..1596)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2256..3047,3048..3116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2879..2898
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3117..3221)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3248..3703
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3335..3354)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3545..3566
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3829..3851
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3843..3860
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3844..3860
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4021..4400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4401..4604
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4555..4575
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4638..4661
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcgatagatc tgatatcggt accagtcgac tctagaggat cccgggtggc atccctgtga
       61 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
      121 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
      181 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
      241 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
      301 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
      361 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
      421 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
      481 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata
      541 ccagcaggag gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca
      601 tttgagttgc ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt
      661 tgaattgggt acgcggccag cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc
      721 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      781 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      841 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      901 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
      961 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1021 caaaaagctc ctcgaggaac tgaaaaacca gaaagttaat tccctatagt gagtcgtatt
     1081 aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     1141 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     1201 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1261 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     1321 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1381 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1441 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1501 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1561 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1621 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1681 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1741 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1801 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1861 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1921 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1981 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2041 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2101 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2161 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2221 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2281 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2341 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2401 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2461 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2521 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2581 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2641 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2701 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     2761 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2821 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2881 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2941 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3001 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3061 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3121 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3181 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3241 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3301 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3361 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3421 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3481 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3541 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3601 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3661 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc
     3721 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     3781 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     3841 gttgtaaaac gacggccagt gccaagctga tctatacatt gaatcaatat tggcaattag
     3901 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     3961 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
     4021 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4081 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     4141 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     4201 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     4261 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     4321 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
     4381 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
     4441 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     4501 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
     4561 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     4621 agaattgatc taccatggac tacaaagacg atgacgacaa gcttgcggcc gacgaattcg
     4681 agactcagtt ccgcagaggg ggtctggggt gcagccctgc cagcatcaag aggaagaaga
     4741 agagggagga ctcaggagac tttggactcc aggtgagcac tatgttctca gaggacgact
     4801 tccagtcaac agaaagagcc ccatatggtc cacaactaca gtggtcccag gatctgccaa
     4861 gagtccaggt ttttagagaa caggccaacc tggaggacag gagtcccagg agaacccaga
     4921 ggatcactgg aggagaacaa gtgctgtggg gccccatcac ccagatattt cccacagttc
     4981 ggcctgctga cctaaccaga gtcatcatgc ctcttgagca aagaagtcag cactgcaagc
     5041 ctgaggaagg ccttcaggcc caagaagaag acctgggcct ggtgggtgca caggctctcc
     5101 aagctgagga gcaggaggct gccttcttct cctctactct gaatgtgggc actctagagg
     5161 agttgcctgc tgctgagtca ccaagtcctc cccagagtcc tcaggaagag tccttctctc
     5221 ccactgccat ggatgccatc tttgggagcc tatctgatga gggctctggc agccaagaaa
     5281 aggaggggcc aagtacctcg cctgacctga tagaccctga gtccttttcc caagatatac
     5341 tacatgacaa gataattgat ttggttcatt tattgctccg caagtatcga gtcaaggggc
     5401 tgatcacaaa ggcagaaatg ctggggagtg tcatcaaaaa ttatgaggac tactttcctg
     5461 agatatttag ggaagcctct gtatgcatgc aactgctctt tggcattgat gtgaaggaag
     5521 tggaccccac tagccactcc tatgtccttg tcacctccct caacctctct tatgatggca
     5581 tacagtgtaa tgagcagagc atgcccaagt ctggcctcct gataatagtc ctgggtgtaa
     5641 tcttcatgga ggggaactgc atccctgaag aggttatgtg ggaagtcctg agcattatgg
     5701 gggtgtatgc tggaagggag cacttcctct ttggggagcc caagaggctc cttacccaaa
     5761 attgggtgca ggaaaagtac ctggtgtacc ggcaggtgcc cggcactgat cctgcatgct
     5821 atgagttcct gtggggtcca agggcccacg ctgagaccag caagatgaaa gttcttgagt
     5881 acatagccaa tgccaatggg agggatccca cttcttaccc atccctgtat gaagatgctt
     5941 tgagagagga gggagaggga gtctgagcat gagatgcaac cagggccagc gggcagggaa
     6001 atgggccaat gcatgcttca gggccacacc cagcagtttc cctgtcctgt gtgaaatcag
     6061 gcccattctt ccctctgtgt ttgatgagag aagtcagtgt tctcagtagt agaaggcaca
     6121 gtgaatggaa gggaacacat tgtatactgc ctttaggttt ctcttccatc gggtgacttg
     6181 gagatttctt tttgtttccc tttggtaatt ttcaaatatt gttcctgtaa taaaagtttt
     6241 agttagcttc aacatctaag tgtatggatg atactgacca cacatgttgt tttgcttatc
     6301 catttcaagt gcaagtgttt gccattttgt aaaacatttt gggaaatctt ccatcttgct
     6361 gtgatttgca ataggtattt tcttggagaa tgtaagaact taacaataaa gctgaactgg
     6421 tgttgtgaaa cagagaaata aaaggagaag gtcatta
//
