LOCUS       sequence-175327-       10540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Gateway-compatible expression vector backbone with an 
            N-terminal Firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10540)
  TITLE     Taipale Lab plasmids
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.2-Firefly-ccdB
FEATURES             Location/Qualifiers
     source          1..10540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..569
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(105..128)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     502..521
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        583..630
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             649..1047
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1134..1367
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1439..1560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1476..1495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1530..1549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1600..1735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1755..1774)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1756..1774)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1784..1801)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1784..1800)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1793..1815)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1942..2397
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2029..2048)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2239..2260
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2423..2527
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2528..2596,2597..3388)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2746..2765)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3559..4147
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4048..4067
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4301..4318
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4435..4456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4471..4476,4477..4494,4495..4501)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4509..4525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4514..4536
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4533..4549
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4533..4549
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4568..4588
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4570..4588
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4616..4842
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4843..5023
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5070..5195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5688..5921
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6106..6150
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        6495..6798
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6799..7002
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6953..6973
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6999..7023
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      7089..7098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7095..8744
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(7161..7179)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    8790..8914
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(8939..8944,8945..8962,8963..8969)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9023..9682
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9089..9108)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10024..10329
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10208..10227
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10370..10494)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(10536..10540,1..37)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgacgatg
       61 gtacctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
      121 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
      181 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
      241 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
      301 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
      361 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
      421 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
      481 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
      541 tctcctcttc ctcatctccg ggcctttcga ctctagacac gtgttgacaa ttaatcatcg
      601 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct
      661 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
      721 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
      781 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
      841 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
      901 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
      961 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     1021 ccctctggtt atgtgtggga gggctaagca caattcgagc tcggtacctt taagaccaat
     1081 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     1141 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     1201 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1261 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1321 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     1381 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     1441 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1501 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1561 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     1621 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     1681 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     1741 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     1801 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1861 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1921 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1981 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     2041 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     2101 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     2161 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     2221 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     2281 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     2341 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     2401 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2461 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2521 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2581 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2641 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2701 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2761 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2821 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2881 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2941 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3001 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3061 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     3121 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     3181 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     3241 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     3301 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     3361 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     3421 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3481 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3541 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3601 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3661 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3721 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3781 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3841 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3901 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3961 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4021 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4081 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4141 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4201 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     4261 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4321 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     4381 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     4441 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     4501 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     4561 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     4621 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     4681 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     4741 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     4801 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     4861 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4921 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4981 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     5041 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     5101 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     5161 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     5221 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     5281 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     5341 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     5401 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     5461 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     5521 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     5581 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     5641 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     5701 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     5761 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     5821 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     5881 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     5941 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     6001 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     6061 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     6121 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     6181 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     6241 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     6301 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     6361 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg atgtcgacgt
     6421 taacgctagc gactgggtct gcgatgtcga cgttaacgct agcgactggg tctgcgataa
     6481 gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     6541 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     6601 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     6661 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     6721 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     6781 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     6841 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     6901 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     6961 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     7021 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg actctagagg
     7081 atccagccgc caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
     7141 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
     7201 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
     7261 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     7321 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     7381 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     7441 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
     7501 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
     7561 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     7621 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
     7681 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
     7741 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
     7801 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     7861 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
     7921 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
     7981 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
     8041 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
     8101 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     8161 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     8221 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
     8281 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     8341 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
     8401 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
     8461 ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg
     8521 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     8581 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     8641 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     8701 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgagtggc ggccgctcga
     8761 gtggctcggg ctcgacctcg ggctcgggca caagtttgta caaaaaagct gaacgagaaa
     8821 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     8881 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     8941 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     9001 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     9061 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     9121 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     9181 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     9241 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     9301 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     9361 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     9421 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     9481 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     9541 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     9601 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     9661 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
     9721 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     9781 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     9841 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     9901 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     9961 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
    10021 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
    10081 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
    10141 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
    10201 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
    10261 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
    10321 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
    10381 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
    10441 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgctct
    10501 atagtaactc gagtctagag ggcccgcggt tcgaaggtaa
//
