LOCUS       sequence-175329-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Ubl4A tagged with FLAG-EGFP at N- terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pEGFP-C1-FLAG-GFP-Ubl4A
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    653..774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(690..709)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     744..763
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(918..939)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgagctgtac aagtccggac tcagatctat gcagctgacg gtgaaggcgc tgcagggccg
       61 cgagtgcagc ctgcaggtgc cagaggacga gctggtgtcc acgctgaagc agctggtctc
      121 cgagaagctg aacgtcccag tgcgccagca gcggctgctg ttcaagggca aggccctggc
      181 agatgggaaa cgactctcgg attatagcat cgggcccaac tccaagctca acctagtggt
      241 caaacccctg gagaaggtgc tactagaaga aggcgaggcc cagaggctgg ccgactcccc
      301 acccccgcag gtctggcagc tgatctccaa agtcttggcc cgccacttca gtgcggcaga
      361 tgccagcagg gtcctggaac agctacagag ggattacgag aggtccctga gtcgcctgac
      421 gctggacgac atcgaacggt tggccagccg cttcctgcac cctgaagtga ctgagacaat
      481 ggagaagggc ttctccaaat aggtcgacgg taccgcgggc ccgggatcca ccggatctag
      541 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
      601 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
      661 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
      721 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
      781 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
      841 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      901 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtgg
//
