LOCUS       sequence-175330-        5870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human Oma1 with C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5870)
  TITLE     Oleh Khalimonchuk Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-Tag4-OMA1
FEATURES             Location/Qualifiers
     source          1..5870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2295..2318
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2371..2390)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2372..2390)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2664..2785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2701..2720)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2755..2774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2792..3247)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2929..2950)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3141..3160
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3274..3378
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3380..3737
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3380..3571
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3403..3423)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3588..3723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3772..4566
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3772..4566
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3826..3845)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4436..4455
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4754..4773)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4798..4845
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5174..5762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5663..5682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgctctagc ccgggcggat ccatgagctt catctgtgga ttgcagtctg
      721 ctgctagaaa ccatgttttc ttccgattta attcactgtc taactggaga aaatgtaaca
      781 cattagcatc cacctcacgg ggctgtcatc aagtacaagt taaccatata gtaaataagt
      841 atcagggact gggagtaaat cagtgtgaca ggtggagttt tctgcctgga aactttcatt
      901 tttatagtac ttttaacaac aaaagaacag gaggcctctc aagtaccaaa agtaaggaaa
      961 tttggaggat taccagcaaa tgtactgtat ggaatgatgc tttttcaaga cagctgctaa
     1021 taaaagaagt tacagcagtc cctagtctgt cagtattgca tcctctaagc cctgcttcca
     1081 taagagctat taggaatttc catacttctc cacggtttca agctgctccg gttcctctct
     1141 tgttgatgat tcttaaacca gtacagaagt tatttgcaat cattgtaggc aggggcataa
     1201 ggaaatggtg gcaggcactt ccccctaaca agaaggaagt agttaaagaa aatataagga
     1261 agaataaatg gaagctattc cttggtttga gtagttttgg attgttattt gtggtgtttt
     1321 attttactca cctggaagta agtccaatca caggaaggag caagctacta ttattgggga
     1381 aagaacagtt cagactttta tcggaactgg aatatgaagc atggatggaa gaatttaaaa
     1441 atgatatgct aactgagaaa gatgcccgat acctggctgt taaagaagtg ctttgtcatc
     1501 taattgaatg caataaagat gttccaggga tctctcagat caattgggtt attcatgtgg
     1561 ttgattcccc aattattaat gccttcgtgc ttccaaatgg acaaatgttt gttttcactg
     1621 gatttttaaa tagtgtaacc gatattcatc aactttcttt ccttctgggc catgaaatag
     1681 cacatgcagt acttgggcat gctgcagaaa aggctggcat ggttcatttg ttggatttcc
     1741 taggtatgat tttcctcaca atgatttggg ccatttgtcc tcgagatagc ttggcacttt
     1801 tgtgccagtg gatacagtct aaattgcagg agtatatgtt taatagacca tacagcagaa
     1861 aattggaggc cgaagctgac aaaattggac tactgcttgc tgcaaaggct tgtgcagaca
     1921 taagagccag ttcagtgttt tggcagcaaa tggagttcgt tgatagcctg catggccaac
     1981 ccaagatgcc agaatggtta tctacacacc cttctcatgg caatcgagtt gagtacttgg
     2041 atagacttat acctcaggct ctcaaaatta gagagatgtg taattgtcca ccactgtcta
     2101 atccagaccc tcgattacta ttcaaactca gcacgaagca ttttcttgaa gaatcagaga
     2161 aagaagacct aaatatcacg aagaaacaga aaatggatac tcttcctatt caaaaacagg
     2221 agcaaatacc attaacatac atagttgaga aaagaacggg cagtggaagc ttatcgatac
     2281 cgtcgacact cgaggattac aaggatgacg acgataagta gggcccggta ccttaattaa
     2341 ttaaggtacc aggtaagtgt acccaattcg ccctatagtg agtcgtatta caattcactc
     2401 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggagatccaa tttttaagtg
     2461 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcac agtcccaagg
     2521 ctcatttcag gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat
     2581 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2641 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2701 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2761 tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa
     2821 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     2881 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     2941 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     3001 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     3061 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     3121 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     3181 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     3241 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3301 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3361 tattgaaaaa ggaagaatcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     3421 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3481 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3541 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3601 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3661 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3721 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     3781 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3841 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3901 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     3961 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     4021 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4081 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4141 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4201 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agaacatcag
     4261 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     4321 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4381 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     4441 gctacccgtg atattgctga agaacttggc ggcgaatggg ctgaccgctt cctcgtgctt
     4501 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4561 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     4621 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4681 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     4741 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     4801 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     4861 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     4921 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     4981 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     5041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5281 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5821 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
//
