LOCUS       sequence-175354-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- 
            terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    426..547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(463..482)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     517..536
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(691..712)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gatctatgca gctgacggtg aaggcgctgc agggccgcga gtgcagcctg caggtgccag
       61 aggacgagct ggtgtccacg ctgaagcagc tggtctccga gaagctgaac gtcccagtgc
      121 gccagcagcg gctgctgttc aagggcaagg ccctggcaga tgggaaacga ctctcggatt
      181 atagcatcgg gcccaactcc aagctcaacc tagtggtcaa acccctggag aaggtgctac
      241 tagaagaagg cgaggcccag aggctggccg actaggtcga cggtaccgcg ggcccgggat
      301 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
      361 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
      421 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      481 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      541 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
      601 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
      661 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
      721 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
      781 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
      841 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
      901 gaa
//
