LOCUS       sequence-17536-e         464 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Schalm SS, Blenis J
  TITLE     Identification of a conserved motif required for mTOR signaling.
  JOURNAL   Curr Biol. 2002 Apr 16. 12(8):632-9.
  PUBMED    11967149
REFERENCE   2  (bases 1 to 464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2002 Apr 16. 12(8):632-9."
COMMENT     Sequence Label: pRK7-HA-S6K1-F5A-E389-R3A
FEATURES             Location/Qualifiers
     source          1..464
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        191..209
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     191..208
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     222..241
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             302..328
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt
       61 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      121 ggcccacccc cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca
      181 tacacatacg atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg
      241 tgtccactcc caggtccaac tgcacctcgg ttctaagctt ctgcaggtcg actctagaat
      301 gtacccatac gatgttccag attacgctgc aggagtggct gacatagacc tggaccagcc
      361 agaggatgca ggctctgagg atgagctgga ggangggggt cagttaaatg aaagcatgac
      421 catgggggga gttggaccat atgaacttgg gcatgggaac attt
//
