LOCUS       sequence-175367-       10863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HIV-1 SIN lentiviral transfer vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard 
            G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, 
            Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, 
            du Pasquier R, Deglon N
  TITLE     The Self-Inactivating KamiCas9 System for the Editing of CNS Disease
            Genes.
  JOURNAL   Cell Rep. 2017 Sep 19;20(12):2980-2991. doi: 
            10.1016/j.celrep.2017.08.075.
  PUBMED    28930690
REFERENCE   2  (bases 1 to 10863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-19- 19"; volume: "20"; issue: "12"; pages: "2980-2991"
COMMENT     Sequence Label: 
            SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE
FEATURES             Location/Qualifiers
     source          1..10863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     120..139
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      202..211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             208..918
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(353..371)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     621..640
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1014..1602
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1067..1087)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1485..1496)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1691..1924
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1949..1967)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1950..1967)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2257..2276)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2490..2509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2609..2631
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2669..2687)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2755..2859
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2860..2928,2929..3720)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3078..3097)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3891..4479
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4380..4399
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4633..4650
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4720..4740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4725..5054
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4905..5040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6188..6253
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6383..6403
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6828..6962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6865..6884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6919..6938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7130..7763
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7810..7935
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8428..8661
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8846..8890
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9157..9274
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9310..9334
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        9360..9574
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     9495..9514
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        9601..9676
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        9721..9969
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9721..9741
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9892..9911
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        9990..10065
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        10359..10434
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(10471..10495)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10518..10534)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10519..10535)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        join(10551..10863,1..187)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10586..10609)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
       61 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      121 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      181 cctttcgacc tctaggatcc cgccaccatg gtgagcaagg gcgaggagga taacatggcc
      241 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      301 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      361 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      421 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      481 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      541 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      601 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      661 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
      721 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
      781 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
      841 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
      901 gacgagctgt acaagtaaag cggccgcgtc gagagatggg ggaggctaac tgagctagct
      961 tagtcatgca ccgggctgca ggaattcgcg gccgcactcg agggaattcc gataatcaac
     1021 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     1081 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     1141 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     1201 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     1261 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     1321 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     1381 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     1441 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     1501 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     1561 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgggaa ttcgagctcg
     1621 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     1681 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     1741 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     1801 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     1861 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1921 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
     1981 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
     2041 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
     2101 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
     2161 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
     2221 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     2281 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     2341 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     2401 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     2461 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2521 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     2581 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     2641 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2701 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2761 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2821 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2881 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2941 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3001 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3061 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3121 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3181 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3241 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3301 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3361 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3421 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3481 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3541 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3601 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3661 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3721 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3781 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3841 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3901 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3961 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4021 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4081 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4141 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4201 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4261 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4321 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4381 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4441 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4501 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4561 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4621 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4681 cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt
     4741 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4801 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4861 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4921 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4981 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5041 taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca
     5101 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
     5161 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
     5221 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
     5281 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
     5341 aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac
     5401 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
     5461 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
     5521 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg
     5581 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
     5641 cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
     5701 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc
     5761 gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
     5821 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
     5881 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac
     5941 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc
     6001 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac
     6061 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt
     6121 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     6181 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     6241 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     6301 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     6361 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     6421 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     6481 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     6541 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
     6601 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
     6661 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
     6721 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     6781 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     6841 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6901 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     6961 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     7021 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     7081 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     7141 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7201 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     7261 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     7321 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     7381 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     7441 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     7501 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     7561 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7621 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7681 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7741 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7801 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7861 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7921 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7981 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     8041 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8101 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     8161 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8221 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     8281 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8341 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8401 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8461 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8521 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8581 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8641 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8701 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8761 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8821 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8881 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8941 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9001 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9061 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     9121 tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg
     9181 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     9241 attacaaaaa caaattacaa aaattcaaaa ttttatcgct agtggatccc ccgggctgca
     9301 ggaattatca caagtttgta caaaaaagca ggcttcatcg atcaattggg atccaattcg
     9361 aacgctgacg tcatcaaccc gctccaagga atcgcgggcc cagtgtcact aggcgggaac
     9421 acccagcgcg cgtgcgccct ggcaggaaga tggctgtgag ggacagggga gtggcgccct
     9481 gcaatatttg catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa atgtctttgg
     9541 atttgggaat cttataagtt ctgtatgaga ccacagatct gaccctggaa aagctgatga
     9601 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     9661 ggcaccgagt cggtgctttt ttagatcgga tccaagcttc catggaaggt cgggcaggaa
     9721 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     9781 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     9841 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     9901 atgcttaccg taacttgaaa gtatttcgat ttcttgggtt tatatatctt gtggaaagga
     9961 cgaaacaccg ctgaagggca tcgacttcag ttttagagct agaaatagca agttaaaata
    10021 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tcatatgcca
    10081 tgggcggccg ctctagactg cagtatttag catgccccac ccatctgcaa ggcattctgg
    10141 atagtgtcaa aacagccgga aatcaagtcc gtttatctca aactttagca ttttgggaat
    10201 aaatgatatt tgctatgctg gttaaattag attttagtta aatttcctgc tgaagctcta
    10261 gtacgataag taacttgacc taagtgtaaa gttgagattt ccttcaggtt tatatagctt
    10321 gtgcgccgcc tgggtaccaa tggagtactt cttgtccagt tttagagcta gaaatagcaa
    10381 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
    10441 ggtactctag actcgagact agtaggcctg acccagcttt cttgtacaaa gtggtgataa
    10501 ttcgatatca agcttatcga taccgtcgac ctcgacgatg gtcgagtacc gggtagggga
    10561 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
    10621 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
    10681 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
    10741 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
    10801 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
    10861 cca
//
