LOCUS       sequence-175368-       10095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To produce lentiviral vector for HTT editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard 
            G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, 
            Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, 
            du Pasquier R, Deglon N
  TITLE     The Self-Inactivating KamiCas9 System for the Editing of CNS Disease
            Genes.
  JOURNAL   Cell Rep. 2017 Sep 19;20(12):2980-2991. doi: 
            10.1016/j.celrep.2017.08.075.
  PUBMED    28930690
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-19- 19"; volume: "20"; issue: "12"; pages: "2980-2991"
COMMENT     Sequence Label: SIN-cPPT-H1-sgHTT1-PGK-mCherry-WPRE
FEATURES             Location/Qualifiers
     source          1..10095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             59..692
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    739..864
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1357..1590
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1775..1819
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2086..2203
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2239..2263
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2289..2503
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2424..2443
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        2530..2605
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(2632..2656)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2679..2695)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2680..2696)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        2712..3211
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2747..2770)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3144..3163
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3226..3235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3232..3942
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3377..3395)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3645..3664
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4038..4626
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4091..4111)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4509..4520)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4715..4948
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4973..4991)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4974..4991)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5281..5300)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5514..5533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5633..5655
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5693..5711)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5779..5883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5884..5952,5953..6744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6102..6121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6915..7503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7404..7423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7657..7674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7744..7764)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7749..8078
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7929..8064
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9212..9277
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9407..9427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9852..9986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9889..9908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9943..9962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
       61 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
      121 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
      181 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
      241 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
      301 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
      361 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
      421 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
      481 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
      541 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      601 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      661 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
      721 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
      781 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
      841 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
      901 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
      961 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     1021 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1081 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     1141 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     1201 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1261 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1321 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1381 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1441 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1501 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1561 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1621 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1681 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1741 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1801 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1861 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1921 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1981 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     2041 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
     2101 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2161 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgcta gtggatcccc
     2221 cgggctgcag gaattatcac aagtttgtac aaaaaagcag gcttcatcga tcaattggga
     2281 tccaattcga acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta
     2341 ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag
     2401 tggcgccctg caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa
     2461 tgtctttgga tttgggaatc ttataagttc tgtatgagac cacagatctg accctggaaa
     2521 agctgatgag ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
     2581 tgaaaaagtg gcaccgagtc ggtgcttttt tagatcggat ccaagctcct gacccagctt
     2641 tcttgtacaa agtggtgata attcgatatc aagcttatcg ataccgtcga cctcgacgat
     2701 ggtcgagtac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     2761 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     2821 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     2881 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     2941 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     3001 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     3061 gctgggaagg ggygggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     3121 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     3181 gttctcctct tcctcatctc cgggcctttc gacctctagg atcccgccac catggtgagc
     3241 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     3301 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     3361 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     3421 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     3481 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     3541 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     3601 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     3661 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     3721 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     3781 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     3841 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     3901 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagcggccg cgtcgagaga
     3961 tgggggaggc taactgagct agcttagtca tgcaccgggc tgcaggaatt cgcggccgca
     4021 ctcgagggaa ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4081 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4141 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4201 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4261 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4321 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4381 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     4441 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4501 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4561 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4621 ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     4681 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     4741 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     4801 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4861 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4921 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
     4981 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     5041 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     5101 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     5161 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
     5221 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     5281 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     5341 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     5401 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     5461 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
     5521 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     5581 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5641 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     5701 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     5761 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5821 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5881 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5941 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6001 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6061 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6121 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6181 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6241 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6301 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6361 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6421 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6481 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6541 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6601 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6661 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6721 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6781 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6841 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6901 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6961 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7021 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7081 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7141 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7201 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7261 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7321 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7381 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7441 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7501 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7561 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7621 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7681 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7741 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7801 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7861 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7921 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7981 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8041 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     8101 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     8161 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     8221 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     8281 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     8341 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
     8401 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     8461 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
     8521 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     8581 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     8641 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     8701 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     8761 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     8821 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     8881 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     8941 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     9001 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
     9061 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
     9121 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     9181 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     9241 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     9301 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     9361 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     9421 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     9481 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     9541 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     9601 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     9661 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     9721 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     9781 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     9841 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9901 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9961 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    10021 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    10081 cctgtgattc ctctg
//
