LOCUS       sequence-175370-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express sgRNA target HUWE1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: HUWE1-sgRNA-1
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     79..98
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        178..253
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        350..635
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 atattagtac aaantacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
       61 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
      121 ttggctttat atatcttgtg gaaaggacga aacaccgata cacctactcc ccgattcgtt
      181 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
      241 accgagtcgg tgcttttttg ttttagagct agaaatagca agttaaaata aggctagtcc
      301 gtttttagcg cgtgcgccaa ttctgcagac aaatggctct agaggtaccc gttacataac
      361 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag
      421 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      481 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      541 gtaaatggcc cgcctggcat tgtgcccagt acatgacctt atgggacttt cctacttggc
      601 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
      661 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
      721 tattttgtgc agcgatgggg gcgggggggg gggggggggg
//
