LOCUS       sequence-175395-        8876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses high levels of WT SaCas9 for genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T
  TITLE     Generation of a SOX2 reporter human induced pluripotent stem cell 
            line using CRISPR/SaCas9.
  JOURNAL   Stem Cell Res. 2017 Jul;22:16-19. doi: 10.1016/j.scr.2017.05.005. 
            Epub 2017 May 17.
  PUBMED    28952927
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2017-07"; volume: "22:16-19. doi"; pages: " 
            10.1016/j.scr.2017.05.005. Epub 2017 May 17"
COMMENT     Sequence Label: CAG-SaCas9-WPRE
FEATURES             Location/Qualifiers
     source          1..8876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1743..1752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1755..1775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1800..4955
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
                     RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
                     SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
                     RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
                     KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
                     IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
                     IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
                     NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
                     VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
                     YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
                     EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
                     VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
                     KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
                     ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
                     DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
                     NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
                     DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
                     RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKG"
     CDS             4956..5003
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5010..5090
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    5125..5713
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5178..5198)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5596..5607)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5803..5822)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5868..5923
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5922..5941)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6284..6300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6284..6300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6297..6319)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6308..6324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6332..6338,6339..6356,6357..6362))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6377..6398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6487..6520
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6954..6971)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7125..7713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7205..7224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7884..8675,8676..8744))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8507..8526
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8745..8849)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgaccg
     1741 gtgccaccat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagcca
     1801 agcggaacta catcctgggc ctggacatcg gcatcaccag cgtgggctac ggcatcatcg
     1861 actacgagac acgggacgtg atcgatgccg gcgtgcggct gttcaaagag gccaacgtgg
     1921 aaaacaacga gggcaggcgg agcaagagag gcgccagaag gctgaagcgg cggaggcggc
     1981 atagaatcca gagagtgaag aagctgctgt tcgactacaa cctgctgacc gaccacagcg
     2041 agctgagcgg catcaacccc tacgaggcca gagtgaaggg cctgagccag aagctgagcg
     2101 aggaagagtt ctctgccgcc ctgctgcacc tggccaagag aagaggcgtg cacaacgtga
     2161 acgaggtgga agaggacacc ggcaacgagc tgtccaccaa agagcagatc agccggaaca
     2221 gcaaggccct ggaagagaaa tacgtggccg aactgcagct ggaacggctg aagaaagacg
     2281 gcgaagtgcg gggcagcatc aacagattca agaccagcga ctacgtgaaa gaagccaaac
     2341 agctgctgaa ggtgcagaag gcctaccacc agctggacca gagcttcatc gacacctaca
     2401 tcgacctgct ggaaacccgg cggacctact atgagggacc tggcgagggc agccccttcg
     2461 gctggaagga catcaaagaa tggtacgaga tgctgatggg ccactgcacc tacttccccg
     2521 aggaactgcg gagcgtgaag tacgcctaca acgccgacct gtacaacgcc ctgaacgacc
     2581 tgaacaatct cgtgatcacc agggacgaga acgagaagct ggaatattac gagaagttcc
     2641 agatcatcga gaacgtgttc aagcagaaga agaagcccac cctgaagcag atcgccaaag
     2701 aaatcctcgt gaacgaagag gatattaagg gctacagagt gaccagcacc ggcaagcccg
     2761 agttcaccaa cctgaaggtg taccacgaca tcaaggacat taccgcccgg aaagagatta
     2821 ttgagaacgc cgagctgctg gatcagattg ccaagatcct gaccatctac cagagcagcg
     2881 aggacatcca ggaagaactg accaatctga actccgagct gacccaggaa gagatcgagc
     2941 agatctctaa tctgaagggc tataccggca cccacaacct gagcctgaag gccatcaacc
     3001 tgatcctgga cgagctgtgg cacaccaacg acaaccagat cgctatcttc aaccggctga
     3061 agctggtgcc caagaaggtg gacctgtccc agcagaaaga gatccccacc accctggtgg
     3121 acgacttcat cctgagcccc gtcgtgaaga gaagcttcat ccagagcatc aaagtgatca
     3181 acgccatcat caagaagtac ggcctgccca acgacatcat tatcgagctg gcccgcgaga
     3241 agaactccaa ggacgcccag aaaatgatca acgagatgca gaagcggaac cggcagacca
     3301 acgagcggat cgaggaaatc atccggacca ccggcaaaga gaacgccaag tacctgatcg
     3361 agaagatcaa gctgcacgac atgcaggaag gcaagtgcct gtacagcctg gaagccatcc
     3421 ctctggaaga tctgctgaac aaccccttca actatgaggt ggaccacatc atccccagaa
     3481 gcgtgtcctt cgacaacagc ttcaacaaca aggtgctcgt gaagcaggaa gaaaacagca
     3541 agaagggcaa ccggacccca ttccagtacc tgagcagcag cgacagcaag atcagctacg
     3601 aaaccttcaa gaagcacatc ctgaatctgg ccaagggcaa gggcagaatc agcaagacca
     3661 agaaagagta tctgctggaa gaacgggaca tcaacaggtt ctccgtgcag aaagacttca
     3721 tcaaccggaa cctggtggat accagatacg ccaccagagg cctgatgaac ctgctgcgga
     3781 gctacttcag agtgaacaac ctggacgtga aagtgaagtc catcaatggc ggcttcacca
     3841 gctttctgcg gcggaagtgg aagtttaaga aagagcggaa caaggggtac aagcaccacg
     3901 ccgaggacgc cctgatcatt gccaacgccg atttcatctt caaagagtgg aagaaactgg
     3961 acaaggccaa aaaagtgatg gaaaaccaga tgttcgagga aaagcaggcc gagagcatgc
     4021 ccgagatcga aaccgagcag gagtacaaag agatcttcat caccccccac cagatcaagc
     4081 acattaagga cttcaaggac tacaagtaca gccaccgggt ggacaagaag cctaatagag
     4141 agctgattaa cgacaccctg tactccaccc ggaaggacga caagggcaac accctgatcg
     4201 tgaacaatct gaacggcctg tacgacaagg acaatgacaa gctgaaaaag ctgatcaaca
     4261 agagccccga aaagctgctg atgtaccacc acgaccccca gacctaccag aaactgaagc
     4321 tgattatgga acagtacggc gacgagaaga atcccctgta caagtactac gaggaaaccg
     4381 ggaactacct gaccaagtac tccaaaaagg acaacggccc cgtgatcaag aagattaagt
     4441 attacggcaa caaactgaac gcccatctgg acatcaccga cgactacccc aacagcagaa
     4501 acaaggtcgt gaagctgtcc ctgaagccct acagattcga cgtgtacctg gacaatggcg
     4561 tgtacaagtt cgtgaccgtg aagaatctgg atgtgatcaa aaaagaaaac tactacgaag
     4621 tgaatagcaa gtgctatgag gaagctaaga agctgaagaa gatcagcaac caggccgagt
     4681 ttatcgcctc cttctacaac aacgatctga tcaagatcaa cggcgagctg tatagagtga
     4741 tcggcgtgaa caacgacctg ctgaaccgga tcgaagtgaa catgatcgac atcacctacc
     4801 gcgagtacct ggaaaacatg aacgacaaga ggccccccag gatcattaag acaatcgcct
     4861 ccaagaccca gagcattaag aagtacagca cagacattct gggcaacctg tatgaagtga
     4921 aatctaagaa gcaccctcag atcatcaaaa agggcaaaag gccggcggcc acgaaaaagg
     4981 ccggccaggc aaaaaagaaa aagggatcct acccatacga tgttccagat tacgcttacc
     5041 catacgatgt tccagattac gcttacccat acgatgttcc agattacgct taagaattca
     5101 agcttcgggg actagtcata tgataatcaa cctctggatt acaaaatttg tgaaagattg
     5161 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5221 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5281 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5341 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5401 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5461 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     5521 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5581 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5641 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5701 gccgcctccc cgcatcggta aattcactcc tcaggtgcag gctgcctatc agaaggtggt
     5761 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     5821 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     5881 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     5941 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     6001 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     6061 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     6121 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     6181 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     6241 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     6301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6361 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6421 ctttccagtc gggaaacctg tcgtgccagc ggatctcaat tccgatcata ttcaataacc
     6481 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctgaa gaggagttta
     6541 cgtccagcca agcttaggat caattctcat gtttgacagc ttatcatcga taagctgatc
     6601 ctcacaggcc gcacccagct tttcttccgt tgccccagta gcatctctgt ctggtgacct
     6661 tgaagaggaa gaggaggggt ctaaggatgt caactacagt cagagaaccc ctttgtgttt
     6721 ggtccccccc cgtgtcacat gtggaacagg gcccagttgg caagttgtac caaccaactg
     6781 aagggattac atgcactgcc ccgcgaagaa ggggcagaga tgtcgtagtc aggtttagtt
     6841 cgtccggggc ggggcatcga tcctctagag tcgacgctag cggatccgct gcattaatga
     6901 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6961 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7021 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7081 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7141 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7201 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7561 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7621 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7681 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7741 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7801 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7861 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7921 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7981 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8041 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8101 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8161 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8221 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8281 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8341 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8401 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8461 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8521 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8581 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8641 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8701 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8761 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8821 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgg
//
